SIMS ion microscopy imaging of boronophenylalanine (BPA) and 13C15N-labeled phenylalanine in human glioblastoma cells: Relevance of subcellular scale observations to BPA-mediated boron neutron capture therapy of cancer

Imaging Mass Spectrometry for Drugs and Metabolites

“…Most of the work done related to the pharmaceutical field has been accomplished by atomic imaging of drugs with SIMS ion microscopy [40][41][42][43][44]. This requires the drug of interest to be isotopically enriched with stable labels or labeled with an atom not natively found in cells or tissues, eliminating the ability to differentiate between a label on the parent or metabolite.…”

“…Imaging results illustrated a heterogeneous distribution of the drug in the tumor, enabling authors to suggest further optimization strategies for use of this therapeutic agent. Chandra et al and Smith et al have used SIMS to study boronphenylalanine-mediated boron neutron capture therapy in human glioblastoma cells injected with 10 B-labeled boronphenylalanine ( 10 BPA) and 13 C 15 n-labeled phenylalanine at 500-nm resolution [41,42,44,46]. The SIMS image in Figure 11.2 illustrates the subcellular distribution of 10 B distribution in freeze-dried human glioblastoma cells after a 2-h treatment with 10 BPA [42].…”

“…Chandra et al and Smith et al have used SIMS to study boronphenylalanine-mediated boron neutron capture therapy in human glioblastoma cells injected with 10 B-labeled boronphenylalanine ( 10 BPA) and 13 C 15 n-labeled phenylalanine at 500-nm resolution [41,42,44,46]. The SIMS image in Figure 11.2 illustrates the subcellular distribution of 10 B distribution in freeze-dried human glioblastoma cells after a 2-h treatment with 10 BPA [42]. Images of intracellular potassium and sodium were obtained to validate that the cells were cryogenically preserved properly prior to analysis.…”

Processing Techniques of Biomaterials Using Ar Cluster Ion Beam for Imaging Mass Spectrometry

“…Matrix assisted laser desorption ionization (MALDI) is a soft ionization technique that has been applied for imaging of biomolecules up to 100 kDa, for instance, proteins in a rat whole body [1]. MALDI is generally applied for the imaging of tissues, because its spatial resolution is more than 10 m. On the other hand, SIMS is applied to cellular imaging, for instance imaging of elemental disposition in animal cells with a spatial resolution of 500 nm [2]. SIMS analysis is generally used for imaging elements, because of the low sensitivities of molecular ions.…”

A Processing Technique for Cell Surfaces Using Gas Cluster Ions for Imaging Mass Spectrometry

“…On the other hand, secondary ion mass spectrometry (SIMS) is applied for cellular imaging, for instance, imaging of elemental disposition in animal cells with a spatial resolution of 500 nm. 2) SIMS analysis is generally used for imaging elements because of the low sensitivities of molecular ions. However, in recent studies, yields of molecular ions have been improved using cluster ions such as liquid metal cluster ions (Au n ῌ , Bi n ῌ ) 3) and C 60 ῌ .…”