Simply translating mercury detection into a temperature measurement: using an aggregation-activated oxidase-like activity of gold nanoparticles

Abstract

<p>A novel Hg2+-responsive thermometer system for translating mercury detection into a temperature monitoring was developed based on an aggregation-activated oxidase-like activity of gold nanoparticles.</p>

Nanozyme-Enabled Analytical Chemistry

“…79 Subsequent research constructed an aggregation-activated oxidase mimic AuNP@β-CD (Figure 5B) to detect the Hg 2+ ions in blood samples. 60 During the detection, the oxidase-like activity of AuNP@β-CD could be activated once Hg 2+ existed. Then, the oxidized TMB yielded heat under the irradiation of a laser.…”

“…So far, nanozyme-enabled detection covers various targets ranging from small molecules, ,− ions − to macromolecules, − cells, , and microorganisms. − And, these versatile in vitro detection targets are summarized in Table S1. Because of the limitation of space, we took the multifunctional nanozyme-enabled in vitro detection, nanozyme powered strips/devices, and newly emerged drug analysis as examples to illustrate recent developments in nanozyme-enabled analytical chemistry in vitro .…”

“…Therefore, a quick response was visualized, and the polymerase chain reaction (PCR) free genetic detection technique was developed by using common thermometers with AuNPs with a sensitivity up to 0.28 nM . Subsequent research constructed an aggregation-activated oxidase mimic AuNP@β-CD (Figure B) to detect the Hg 2+ ions in blood samples . During the detection, the oxidase-like activity of AuNP@β-CD could be activated once Hg 2+ existed.…”

“…Copyright 2020 American Chemical Society. B–F reprinted with permission from ref . Copyright 2020 Royal Society of Chemistry.…”

Integration and Quantitative Visualization of 3,3′,5,5′-Tetramethylbenzidine-Probed Enzyme-Linked Immunosorbent Assay-like Signals in a Photothermal Bar-Chart Microfluidic Chip for Multiplexed Immunosensing

“…In comparison with the volumetric bar-chart chips, the photothermal bar-chart chips offer (1) simpler and lower cost chip manufacturing (e.g., without complicated microfluidic patterning on glass matrixes using cleanroom facilities); (2) easier controllability toward the bar-chart pumping performance by remotely manipulating the irradiation parameters; and (3) higher compatibility with a variety of commercial ELISA kits using TMB-based chromogenic substrates (e.g., no need to prepare additional specific materials, such as catalase- or platinum NP-conjugated antibodies). Given that TMB is one of the most popular chromogenic substrates in various bioanalytical settings, ,− a wide range of these conventional bioassays can be flexibly introduced in the platform to implement diverse lab-on-a-chip biosensing tasks.…”