Simplified Stereological Evaluation of Renal Morphology after Unilateral Ureteral Obstruction

Abstract

Objective and methods: This study established a simple stereological method to obtain quantitative information about two- or three-dimensional structures based on observations from kidney sections in the unilateral ureteral obstruction(UUO) model. Results: Tubulointerstitial area(TA) and TA/the area of a rectangular field(RA) were raised gradually, but significantly, in the obstructed kidney from 1 to 3months post-ligation in comparison to the sham kidney of sham-operated rats(SOR). On the contrary, glomerular area(GA) and glomerular volume(GV) were decreased progressively over time, but significantly, in the obstructed kidney from 3weeks to 3months post-ligation compared to the sham kidney of SOR. UUO caused a progressive decline of TA and TA/RA in the contralateral kidney. More specifically, there were significant decreases in TA at 1,2,3months post-ligation, while in TA/RA only at 3months post-ligation in comparison to the right kidney of SOR. In contrast, GA and GV enhanced in a time-dependent manner in the contralateral kidney, in which the difference in GA reached significance only at 3months post-ligation, whereas the difference in GV reached significance from 1 to 3months post-ligation when comparing with the right kidney of SOR. Conclusions: Our results confirmed two typical features of obstructive nephropathy, including widen interstitial space and glomerular atrophy in the obstructed kidney, and compensatory growth of the contralateral kidney.

Exogenous C-type natriuretic peptide infusion ameliorates unilateral ureteral obstruction-induced tubulointerstitial fibrosis in rats

“…40 In addition, our previous study found that the glomerular size gradually enhanced in a time-dependent manner in the contralateral kidneys, which is capable of Exogenous CNP infusion ameliorates UUO-induced TIF P Hu et al compensating for the damaged renal unit of the obstructed kidneys. 20 However, in this study, UUO rats exhibited significant higher BUN and Cr than those in the shamoperation group from 2 weeks to 1 month after ligation; therefore, we believe that systemic inflammation and oxidative stress generated by UUO may mainly contribute to renal damage. 41,42 In this study, in addition to renal protective effects, CNP infusion could also alleviate these above pathological lesions in the obstructed kidneys.…”

“…Glomerular volume (GV) was as follows: GV ¼ 4pr max 3 /3 (Figure 1b). 20 Real-Time PCR Total RNA was extracted from renal tissue using TRIzol reagent (Invitrogen, USA). Ultraviolet spectrophotometer measuring absorbance and agarose gel electrophoresis confirmed that there had been no degradation of RNA.…”

he Effects of High Dose Sugammadex on Rat Kidney Tissue Following Unilateral Ureteral Obstruction

“…Obstructive nephropathy is one of the most common causes of renal insufficiency in children and adults. Also proportion of patients with known obstructive nephropathy -with or without renal failure-undergoing different surgical procedures under general anesthesia is nonignorable Unilateral ureteral obstruction (UUO) is commonly used model showed to imitate the process of obstructive nephropathy in a feasible, fast and species-independent manner (1,2). Peritubular capillaries rarefaction, tubular atrophy, inflammatory infiltration, widen interstitial space, and progressive tubulointerstitial fibrosis are important morphological features of obstructed kidneys (3,4).…”