Simplified purification of soluble histidine-tagged green fluorescent protein from cocoon of transgenic silkworm in metal affinity hydroxyapatite chromatography

Sugo, Ken; Yoshitake, Tomohiko; Tomita, Masahiro; Kobayashi, Shintaro; Kurosawa, Yae; Kawamura, Katsumi; Okuyama, Tsuneo

doi:10.1016/j.seppur.2010.11.015

Cited by 8 publications

(4 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydroxyapatite (HAP) is considered as an important mineral phase due to its significance as a biomineral and its various industrial and medical applications. [1][2][3][4][5][6][7] Many pathways have been reported to synthesize hydroxyapatite, namely dry processes, 8,9 sol-gel, [10][11][12][13] spray pyrolysis, 14,15 emulsion system, 16 solvothermal synthesis, 17 sonochemical synthesis 18 and others. 19,20 However, the co-precipitation approach is often used because it is easy to control and it may be adapted as a pseudo sol-gel method by the addition of surfactants.…”

Section: Introductionmentioning

confidence: 99%

Microwave-assisted synthesis of mesoporous nano-hydroxyapatite using surfactant templates

et al. 2014

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Microwave-assisted synthesis of mesoporous nano-hydroxyapatite using surfactant templates

et al. 2014

View full text Add to dashboard Cite

“…As a key chromatographic technology, immobilized metal affinity chromatography (IMAC) has become important tool for protein identification, detection, separation, and purification. The latest progress in theoretical research and applications of IMAC have been extensively reviewed . However, the leakage of metal ion from IMAC column is the fatal weakness of this method during application process, which results in short column lifetime, poor reproducibility, serious contamination to products and a detector .…”

Section: Introductionmentioning

confidence: 99%

Evaluation and optimization of the metal‐binding properties of a complex ligand for immobilized metal affinity chromatography

Chen

et al. 2016

J of Separation Science

View full text Add to dashboard Cite

The simultaneous determination of two binding parameters for metal ions on an immobilized metal affinity chromatography column was performed by frontal chromatography. In this study, the binding parameters of Cu(2+) to l-glutamic acid were measured, the metal ion-binding characteristics of the complex ligand were evaluated. The linear correlation coefficients were all greater than 99%, and the relative standard deviations of two binding parameters were 0.58 and 0.059%, respectively. The experiments proved that the frontal chromatography method was accurate, reproducible, and could be used to determine the metal-binding parameters of the affinity column. The effects of buffer pH, type, and concentration on binding parameters were explored by uniform design experiment. Regression, matching and residual analyses of the models were performed. Meanwhile, the optimum-binding conditions of Cu(2+) on the l-glutamic acid-silica column were obtained. Under these binding conditions, observations and regression values of two parameters were similar, and the observation values were the best. The results demonstrated that high intensity metal affinity column could be effectively prepared by measuring and evaluating binding parameters using frontal chromatography combined with a uniform design experiment. The present work provided a new mode for evaluating and preparing immobilized metal affinity column with good metal-binding behaviors.

show abstract

“…Many studies reported the applicability of affinity tags and target proteins for the purpose of site‐specific and oriented protein immobilizations. The polyhistidine tag (his‐tag) was a popular choice for the immobilization and purification of proteins with metal affinity resins …”

Section: Introductionmentioning

confidence: 99%

“…In contrast, several site‐specific immobilization methods through affinity peptide tags would offer advantages by effectively retaining biological activity by maintaining the conformation of the adsorbed protein molecules. Various affinity peptide tag/ligand, such as biotin‐avidin, anti‐GST antibody, or His 6 ‐Ni were utilized for the site‐specific immobilization and for the purification of target proteins. Many studies reported the applicability of affinity tags and target proteins for the purpose of site‐specific and oriented protein immobilizations.…”

Section: Introductionmentioning

confidence: 99%

Site‐specific reversible immobilization and purification of His‐tagged protein on poly(2‐acetamidoacrylic acid) hydrogel beads

Kim

Park

et al. 2012

Polymers for Advanced Techs

View full text Add to dashboard Cite

Ni2+‐complexed poly(2‐acetamidoacrylic acid) (PAAA) hydrogel beads were developed for the site‐specific reversible immobilization and purification of the histidine‐tagged green fluorescent protein (His‐tagged GFP). PAAA hydrogel beads were prepared by photopolymerization, and significantly improved mechanical properties of PAAA hydrogel beads were observed in comparison with PAAA hydrogel from our previous study. Confocal laser scanning microscopy was used to determine the binding of His‐tagged GFP to the hydrogel beads in three‐dimensional space. Photoluminescence spectroscopy revealed 89% of binding efficiency of His‐tagged GFP to the Ni2+‐PAAA hydrogel beads, 51% of yielding recovery. The maximum binding capacity of His‐tagged GFP was estimated to be 0.45 µg/mg of Ni2+‐PAAA hydrogel beads. The recombinant His‐tagged GFP from the soluble fraction of E. coli BL21(DE3) cell lysates was purified with Ni2+‐PAAA hydrogel beads. The major advantage of the Ni2+‐PAAA hydrogel beads system was simple preparation procedures of producing the matrix, because PAAA hydrogel beads had relatively enhanced mechanical strength than soft hydrogels. Copyright © 2012 John Wiley & Sons, Ltd.

show abstract

Simplified purification of soluble histidine-tagged green fluorescent protein from cocoon of transgenic silkworm in metal affinity hydroxyapatite chromatography

Cited by 8 publications

References 7 publications

Microwave-assisted synthesis of mesoporous nano-hydroxyapatite using surfactant templates

Microwave-assisted synthesis of mesoporous nano-hydroxyapatite using surfactant templates

Evaluation and optimization of the metal‐binding properties of a complex ligand for immobilized metal affinity chromatography

Site‐specific reversible immobilization and purification of His‐tagged protein on poly(2‐acetamidoacrylic acid) hydrogel beads

Contact Info

Product

Resources

About