Simplified modification of the macrophage migration inhibition method in mice

Kinetics of production of macrophage migration inhibition factor in mixed cultures of lymphocytes and tumor cells

“…Re From the Ist through the 4th day of incubation the contents of the individual wells were removed, the cells were separated by centrifugation at 1000g for 30 min, and the resuiting supernatants were tested on peritoneal macrophages of normal B6 mice in a micromodification of the macrophage migration inhibition test [4]. The results were assessed as the macrophage migration inhibition index (MII, %), calculated by the equation: MII = (a--b)/ a x 100%, where a and b are the mean weight of the projection of the migration zone on paper in the syngeneic control and experiment respectively.…”

Specific Suppressor T‐Cells Immune to Antigens of the H‐2 Complex: Receptors, Clonal Structure, Genetic Restriction and Antigenic Markers

“…MHC-speciiic splenic CTL and MIF-producers were assayed in vitro 10-14 days after i/p priming with 2.5 x 10'' EL-4 leukemia cells indigenous to B6 (H-2^) mice. MIF-producers were assessed by a micromodification of the capillary method (Suslov et al 1979).…”