Simplified conditions for storing and cryopreservation of dental pulp stem cells

Human Dental Pulp Stem Cells – A Prospective Regenerative Therapy

“…When the blocks of the hydroxyapatite/tricalcium phosphate (HA/TCP) containing SCAP and PDLSC are inserted into the socket of swine it led to the regeneration of the root/PDL complex, on top of which an artificial dental crown can be affixed. By this way, engineered bioroots showed lower compressive strength than that of the natural swine root dentin, but it is noteworthy that they resulted in normal functioning devoid of major hindrance and very well adjusted the porcelain crown (10).…”

“…The technique basically involves the use of ex vivo expanded cells grown on a support of biocompatible material under appropriate environmental conditions in order to create tissue replacement and living prostheses (10).…”

“…There are various stem cell banks, storages that preserve and maintain ethically sourced stem cell obtained from different origins. The "International Society for Stem Cell Research and the International Stem Cell Banking Initiative" is one of the pioneers for this program (10). In India, "Life Cell International", was the first private sector stem cell banking services started in Bangalore (India) in the year 2009.…”

Cryopreservation of Dental Stem Cells

“…Current studies have shown significantly higher post-thaw cell growth and viability using those methods, in contrast with the rapid freezing method described below. For instance, Huynh et al concluded that controlled-rate freezing methods resulted in a significantly higher number of viable cells, both in 5% and 10% DMSO (79.7% and 79.0% respectively) compared with the rapid freezing method (41). At the same time, Naaldijk et al observed the effect of different freezing rates during cryopreservation of rat mesenchymal stem cells and they determined, on the other hand, that the rapid freezing protocol is no less effective in maintaining post-thaw viability of MSC compared to the controlled rate freezing method.…”

“…Regardless the percentage of DMSO used, DPSCs cryopreserved for 6 months by the latter method did not regrow after thawing. In comparison to it, the former method resulted in significant higher number of viable cells and the more rapid growth rate (47). Unfortunately, this approach also seems to be unsuccessful in cryopreservation of whole intact teeth, due to progressive root resorption and the damage of DSCs embedded in the hard dental tissues (48).…”