Simplified Cellular Grafting for Treatment of Vitiligo and Piebaldism

Goh, Boon Kee; Chua, Xuey‐Mii; Chong, Kai‐Ling; Mil, Martine De; Geel, Nanny A. C. Van

doi:10.1111/j.1524-4725.2009.01423.x

Cited by 22 publications

(23 citation statements)

References 9 publications

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“…To further simplify the laboratory procedure, one could also use the simplified cellular grafting technique described by Goh et al . [10] This modification reduces cell preparation time, amount of reagents needed and costs, and obviates the need of a laboratory for extraction of cells. With this technique, the extraction of epidermal cells from the donor skin is performed by using an inexpensive 6-well plate, a microfilter, and 3 reagents: trypsin, soybean trypsin inhibitor, and PBS.…”

Section: Methods For Data Collectionmentioning

confidence: 99%

A review of non-cultured epidermal cellular grafting in vitiligo

Geel

Goh

Wallaeys

et al. 2011

J Cutan Aesthet Surg

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Section: Methods For Data Collectionmentioning

confidence: 99%

A review of non-cultured epidermal cellular grafting in vitiligo

Geel

Goh

Wallaeys

et al. 2011

J Cutan Aesthet Surg

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“…These simplified techniques include the ReCell kit (Recell, Clinical Cell Culture, Cambridge, UK), 17 which is a single-use battery-operated autologous cellharvesting device, and the 6-or 4-well plate methods, which only requires a laboratory dish divided into 6 or 4 compartments, respectively, and 2 to 3 reagents (phosphate-buffered saline and trypsin with or without soybean trypsin inhibitors). 18,19 Although these innovative techniques simplify and reduce time required in the cell preparation process, the concentration of viable cells extracted, when compared with the traditional extraction method, is yet to be determined.…”

Section: Resultsmentioning

confidence: 99%

Twelve-month and sixty-month outcomes of noncultured cellular grafting for vitiligo

Gan

Kong

Tan

et al. 2016

Journal of the American Academy of Dermatology

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“…[15] Recently, Goh et al have simplified the extraction of epidermal cells from donor skin using a simple inexpensive six-well plate, a microfilter, and only three reagents: trypsin, trypsin inhibitor (soybean), and phosphate buffered saline (PBS). [16] DISCUSSION Non-cultured epidermal suspension is indicated mainly for segmental vitiligo and for stable nonsegmental vitiligo that does not respond to medical treatment. For the assessment of stability, Parsad and Gupta [17] suggested as definition the absence of progression of the disease for the past one year.…”

Section: Modifications Of the Initial Techniquementioning

confidence: 99%

Non-cultured epidermal suspension in vitiligo: From laboratory to clinic

Gauthier¹,

Benzekri²

2012

Indian J Dermatol Venereol Leprol

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Background: Medical treatments are ineffective in many patients and surgical methods have therefore been developed. Objective: A review of autologous non-cultured melanocyte grafting techniques is proposed to obtain a successful repigmentation of vitiligo macules. Methods: Initially in 1992, we had developed a simplified grafting method which was carried out in the following two steps: production of blisters on the depigmented lesions by freezing with liquid nitrogen and injection in each blister of a non-cultured suspension of epidermal cells. The cellular suspension was obtained from samples of skin of the hair scalp after trypsinization. This very simple technique could be used at the dermatologist's clinic. Since 1998 (Olsson MJ, Juhlin L), quite comparable but improved and more sophisticated techniques have been proposed for the surgical treatment of vitiligo. These techniques require a laboratory set up to perform the melanocyte transplantation. The donor zone was usually taken on the gluteal region. The time of trypsinization was reduced to 60 minutes at 37°C and the centrifuged cellular suspension added with hyaluronic acid (Van Geel) was directly applied on a dermabraded or laser abraded vitiligo lesions. Results: Whatever the technique chosen, repigmentation was evident within 25 to 30 days. Coalescence of the pigmented areas was spontaneously observed or obtained after UVB radiation. It is obvious that the complete repigmentation occurred more rapidly with the recent techniques compared with the initial method, but the efficiency was quite similar. Conclusion: The use of non-cultured epidermal suspension appears to be an effective, safe, and simple method for treating patients with achromic areas lacking melanocytes.

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Simplified Cellular Grafting for Treatment of Vitiligo and Piebaldism

Abstract: This set-up is simple and inexpensive; it reduces cell preparation time, amount of reagents used, and costs, and obviates the need of a laboratory for extraction of epidermal cells.

Cited by 22 publications

References 9 publications

A review of non-cultured epidermal cellular grafting in vitiligo

A review of non-cultured epidermal cellular grafting in vitiligo

Twelve-month and sixty-month outcomes of noncultured cellular grafting for vitiligo

Non-cultured epidermal suspension in vitiligo: From laboratory to clinic

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