Simplified aptamer-based colorimetric method using unmodified gold nanoparticles for the detection of carcinoma embryonic antigen

Luo, Cheng Hung; Wen, Wei; Lin, Fenge; Zhang, Xun; Gu, Haoshuang; Wang, Shengfu

doi:10.1039/c4ra14833a

Cited by 50 publications

(20 citation statements)

References 34 publications

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“…Dumani et al designed AuNPs coated with glycol-chitosan that can be taken up by immune cells and subsequently transported to the sentinel lymph node to detect metastases [121]. AuNPs can modified with nucleotide sequence for detecting [127], imaging [25] and therapy [26].…”

Section: Deliverymentioning

confidence: 99%

The Basic Properties of Gold Nanoparticles and their Applications in Tumor Diagnosis and Treatment

Bai

Wang

Song

et al. 2020

IJMS

250

156

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Gold nanoparticles (AuNPs) have been widely studied and applied in the field of tumor diagnosis and treatment because of their special fundamental properties. In order to make AuNPs more suitable for tumor diagnosis and treatment, their natural properties and the interrelationships between these properties should be systematically and profoundly understood. The natural properties of AuNPs were discussed from two aspects: physical and chemical. Among the physical properties of AuNPs, localized surface plasmon resonance (LSPR), radioactivity and high X-ray absorption coefficient are widely used in the diagnosis and treatment of tumors. As an advantage over many other nanoparticles in chemicals, AuNPs can form stable chemical bonds with S-and N-containing groups. This allows AuNPs to attach to a wide variety of organic ligands or polymers with a specific function. These surface modifications endow AuNPs with outstanding biocompatibility, targeting and drug delivery capabilities. In this review, we systematically summarized the physicochemical properties of AuNPs and their intrinsic relationships. Then the latest research advancements and the developments of basic research and clinical trials using these properties are summarized. Further, the difficulties to be overcome and possible solutions in the process from basic laboratory research to clinical application are discussed. Finally, the possibility of applying the results to clinical trials was estimated. We hope to provide a reference for peer researchers to better utilize the excellent physicochemical properties of gold nanoparticles in oncotherapy.

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Section: Deliverymentioning

confidence: 99%

The Basic Properties of Gold Nanoparticles and their Applications in Tumor Diagnosis and Treatment

Bai

Wang

Song

et al. 2020

IJMS

250

156

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“…Compared to other reported methods of detection of CEA, the proposed method shows relatively excellent linear range, and detection limit, as shown in Fig. 5 32 33 34 35 36 .…”

Section: Resultsmentioning

confidence: 86%

Paper-based upconversion fluorescence resonance energy transfer biosensor for sensitive detection of multiple cancer biomarkers

Dong

Zhou

et al. 2016

Sci Rep

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A paper-based upconversion fluorescence resonance energy transfer assay device is proposed for sensitive detection of CEA. The device is fabricated on a normal filter paper with simple nano-printing method. Upconversion nanoparticles tagged with specific antibodies are printed to the test zones on the test paper, followed by the introduction of assay antigen. Upconversion fluorescence measurements are directly conducted on the test zones after the antigen-to-antibody reactions. Furthermore, a multi-channel test paper for simultaneous detection of multiple cancer biomarkers was established by the same method and obtained positive results. The device showed high anti-interfere, stability, reproducible and low detection limit (0.89 ng/mL), moreover it is very easy to fabricate and operate, which is a promising prospect for a clinical point-of-care test.

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“…This improvement in sensitivity is likely due to the presence of fewer negatively charged DNA-based aptamers on the surface of the AuNP that reduces the repulsion and facilitates aggregation aer analyte binding. 22,[29][30][31] Also, the packing density of the aptamers play an important role in the aptamer-analyte binding. 32,33 The higher packing density might interfere with adequate aptamer folding, thus affecting the aptamer-analyte interaction.…”

Section: Discussionmentioning

confidence: 99%

Aptamer–gold nanoparticle conjugates for the colorimetric detection of arboviruses and vector mosquito species

et al. 2019

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Simplified aptamer-based colorimetric method using unmodified gold nanoparticles for the detection of carcinoma embryonic antigen

Abstract: A new colorimetric aptasensor for the detection of CEA was developed. Aptamer-based colorimetric method with nanoparticles was used for the detection of CEA. The colorimetric aptasensor has potential for the detection of other proteins or nucleotides.

Cited by 50 publications

References 34 publications

The Basic Properties of Gold Nanoparticles and their Applications in Tumor Diagnosis and Treatment

The Basic Properties of Gold Nanoparticles and their Applications in Tumor Diagnosis and Treatment

Paper-based upconversion fluorescence resonance energy transfer biosensor for sensitive detection of multiple cancer biomarkers

Aptamer–gold nanoparticle conjugates for the colorimetric detection of arboviruses and vector mosquito species

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