Simplification of the purification of heat stable recombinant low molecular weight proteins and peptides from GST-fusion products

Sakhel, Beatrice; Jayanthi, Srinivas; Muhoza, Djamali; Okoto, Patience; Kumar, Thallapuranam Krishnaswamy Suresh; Adams, Paul

doi:10.1016/j.jchromb.2021.122627

Cited by 7 publications

(1 citation statement)

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“…After enzymatic cleavage with selective heat-induced precipitation of (Glutathione-S-transferase) GST-affinity tag the peptides are purified. Because of the limitaions like coupling efficiency, steric hindrance of protective groups or larger side chains and the tendency of peptides to aggregate results in low yields [3]. The peptides synthesised by conventional method requires more time and thus an alternative implement like automated microwave technology offers advantages like higher yeilds and less reaction time.…”

Section: Introductionmentioning

confidence: 99%

A Review on Peptide Synthesis: Therapeutic agents, stabilizing agents and its applications

T¹,

Khaleel²,

Ramesh³

et al. 2023

J Integral Sci

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Peptides are required as a part of health through their extended activity. Peptides are also purified because of their high target affinity. The synthesis includes both the conventional method and the microwave method among which microwave procedure is considered superior due to less reaction time and higher yeilds. The peptides were assessed for the inhibition activity of Urokinase plasminogen (UpA), coagulation factor XII, and also for the cytotoxicity and anti-microbial activity.

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Section: Introductionmentioning

confidence: 99%