Simplification and optimization of media ingredients for enhanced production of CMCase by newly isolatedBacillussubtilisNA15

Abstract

Cellulose degrading bacterium was isolated from composting biomass and identified as Bacillus subtilis on the basis of phenotypic fingerprint using GEN III MicroPlateTM and 16S ribosomal RNA (rRNA) gene sequence analysis. 16S rRNA gene sequence similarity analysis showed 99% similarity with Bacillus subtilis. The screening of 11 nutrients was done using Plackett‐Burman design to explicate the parameters that significantly influence the carboxy methyl cellulase (CMCase) production. Maximum CMCase production of 0.47 U/mL was obtained using response surface methodology with carboxy methyl cellulose (CMC): 18 g/L, peptone: 5 g/L, yeast extract: 5 g/L and MnCl2: 0.5 g/L. The model predicted the maximum CMCase activity (0.45 U/mL), which was in good agreement with the experimental value of 0.47 U/mL showing sevenfold increase as compared to unoptimized medium. Presence of MnCl2 in the medium significantly enhanced the CMCase production. © 2014 American Institute of Chemical Engineers Environ Prog, 34: 533–541, 2015

A novel Bacillus sp. with rapid growth property and high enzyme activity that allows efficient fermentation of soybean meal for improving digestibility in growing pigs

“…Bacillus spp. are known for producing a plethora of hydrolytic enzymes (Akhtar et al, 2015;Ho & Ku, 2017;Thite & Nerurkar, 2015;Zhang et al, 2018). Promising level of cellulase (0.07 U ml −1 ), xylanase (1.91 U ml −1 ), and amylase (2.66 U ml −1 ) was observed in cell-free supernatant of overnight grown B. subtilis CP-9.…”

“…Over the years, bacterial fermentation is being preferred over fungal due to fast growth and simple downstream processing. Among different genus of bacteria, species of bacilli are known to produce extracellular cellulolytic (Akhtar et al, 2015;Ho & Ku, 2017;Zhang et al, 2018), hemicellulolytic (Seo et al, 2013;Ho & Ku, 2017), and proteolytic (Zhang et al, 2018) enzymes, which helps in the biodegradation of allergenic proteins and NSP. A combination of B. subtilis, Hansenula anomala, and Lactobacillus casei was used for SBM fermentation to improve the growth performance, nutrient digestibility, and microbial flora in piglets (Yuan et al, 2017).…”

Prospecção, clonagem, produção, purificação e caracterização de enzimas do sistema lignocelulolítico de Bacillus licheniformis CBMAI 1609

“…Quando o micro-organismo foi cultivado no meio de cultura otimizado (1,8 % de CMC, 0,8 % de peptona e 0,48 % extrato de levedura (m/v)) esses autores obtiveram atividade endoglucanásica de 0,43 U/mL. Em outro estudo, Akhtar et al (2015) também otimizaram a produção de endoglucanase pela linhagem B. subtilis NA15 e obtiveram atividade enzimática de 0,47 U/mL após 24 horas de fermentação a 37 ºC e 120 rpm em meio de cultura otimizado (1,8 % de CMC, 0,5 % de peptona, 0,5 % de extrato de levedura e 0,05 % de MnCl 2 (m/v)).…”