Simpler flame photometric determination of erythrocyte sodium and potassium: the reference range for apparently healthy adults.

Abstract

Separated erythrocytes are washed repeatedly with iso-osmolar magnesium chloride solution, lysed by adding saponin, and sodium potassium measured in the diluted hemolysate by flame photometry. The coefficient of variation for the method was less than 4%. Reference intervals determined for a healthy population and hospitalized (elective surgery) patients without electrolyte disorders were 4.6-7.8 mmol/liter for erythrocyte sodium concentration and 94-110 mmol/liter for erythrocyte potassium concentration (2.5-97.5 percentiles).

A high-sensitivity HPLC assay for measuring intracellular Na+ and K+ and its application to Plasmodium falciparum infected erythrocytes

“…In initial experiments the method was applied to uninfected human erythrocytes, yielding a Na + content of 6.2 ± 0.3 mmol/10 13 cells and a K + content of 99 ± 5 mmol/10 13 cells (n = 6, mean ± SD) ( Fig. 1A ), consistent with well-established literature values 12 . Application of the method to human erythrocytes infected with the mature asexual trophozoite form of the malaria parasite, Plasmodium falciparum (approximately 36 h post-invasion), confirmed the long-recognised 13 16 17 changes in the Na + and K + content of the infected cells, with Na + estimated here to have increased to 52.6 ± 2.8 mmol/10 13 cells (n = 6, mean ± SD, P < 0.01, paired t-test) and K + estimated here to have decreased to 70 ± 4 mmol/10 13 cells (n = 6, mean ± SD, P = 0.01, paired t-test) ( Fig.…”

“…Screening compounds for their ability to inhibit cell ion transport mechanisms and thereby disrupt transmembrane electrochemical gradients has generally involved low- to medium-throughput methodologies based on such techniques as flame photometry or atomic absorption spectrometry 12 13 , fluorescent ion-sensitive dyes 7 8 9 10 11 , or ion-sensitive electrodes 14 or microelectrodes 15 . These methods are typically limited to the analysis of single ions and/or require large sample volumes/cell numbers and/or complex sample preparation or experimental manoeuvres.…”

Indapamide (Natrilix): the agent of choice in the treatment of recurrent renal calculi associated with idiopathic hypercalciuria

“…2; although there was a significant decrease in urinary calcium excretion using 25 and 50 mg of HCT Boehringer Mannheim kit [16,17]. Urinary plasma, and red blood cell sodium and potassium, were measured (P<0.05 and P<0.001, respectively), the decrease with 25 mg was sub-optimal and with 12.5 mg did not reach using flame photometry [18] and magnesium was measured using atomic absorption spectrophotometry [19]. statistical significance.…”

Primary red cell hydration disorders: Pathogenesis and diagnosis

“…Several techniques enable the measurement of intracellular ionic content. Oldest techniques were based on absorption spectrophotometry . Ion‐sensitive fluorescent indicators are also available, using fluorescence microscope evaluation.…”