Simple yet effective: Microbial and biotechnological benefits of rumen liquid addition to lignocellulose-degrading biogas plants

Nagler, Magdalena; Kozjek, Katja; Etemadi, Mohammad; Insam, Heribert; Podmirseg, Sabine Marie

doi:10.1016/j.jbiotec.2019.05.004

Cited by 32 publications

(15 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of AF specific primers targeting the LSU region have been published (Dollhofer et al, 2016;Nagler et al, 2018), and employed to study AF community composition of environmental samples (Dollhofer et al, 2017;Nagler et al, 2019). However, a sufficiently large reference database for these taxonomic marker genes from previously characterized taxa and their type specimens is still lacking (Wang et al, 2017).…”

Section: Miscellaneous Features Referencesmentioning

confidence: 99%

Anaerobic Fungi: Past, Present, and Future

et al. 2020

View full text Add to dashboard Cite

Anaerobic fungi (AF) play an essential role in feed conversion due to their potent fiber degrading enzymes and invasive growth. Much has been learned about this unusual fungal phylum since the paradigm shifting work of Colin Orpin in the 1970s, when he characterized the first AF. Molecular approaches targeting specific phylogenetic marker genes have facilitated taxonomic classification of AF, which had been previously been complicated by the complex life cycles and associated morphologies. Although we now have a much better understanding of their diversity, it is believed that there are still numerous genera of AF that remain to be described in gut ecosystems. Recent markergene based studies have shown that fungal diversity in the herbivore gut is much like the bacterial population, driven by host phylogeny, host genetics and diet. Since AF are major contributors to the degradation of plant material ingested by the host animal, it is understandable that there has been great interest in exploring the enzymatic repertoire of these microorganisms in order to establish a better understanding of how AF, and their enzymes, can be used to improve host health and performance, while simultaneously reducing the ecological footprint of the livestock industry. A detailed understanding of AF and their interaction with other gut microbes as well as the host animal is essential, especially when production of affordable high-quality protein and other animal-based products needs to meet the demands of an increasing human population. Such a mechanistic understanding, leading to more sustainable livestock practices, will be possible with recently developed-omics technologies that have already provided first insights into the different contributions of the fungal and bacterial population in the rumen during plant cell wall hydrolysis.

show abstract

Section: Miscellaneous Features Referencesmentioning

confidence: 99%

Anaerobic Fungi: Past, Present, and Future

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Recently, the addition of rumen liquid has been found to improve the efficiency of biogas production by anaerobic fungi typical of grazing animal digestive tracts (Gruninger et al, 2014;Nagler, Kozjek, Etemadi, Insam, & Podmirseg, 2019).…”

Section: Fung I Enhan Cing Pl Ant-ba S Ed B Ioenergy Produc Tionmentioning

confidence: 99%

“…The basidiomycete white‐rot fungi are the most widely used for delignification of bioenergy feedstocks, as they completely mineralize lignin in aerobic conditions (Saritha & Arora, 2012). Recently, the addition of rumen liquid has been found to improve the efficiency of biogas production by anaerobic fungi typical of grazing animal digestive tracts (Gruninger et al, 2014; Nagler, Kozjek, Etemadi, Insam, & Podmirseg, 2019).…”

Section: Fungi Enhancing Plant‐based Bioenergy Productionmentioning

confidence: 99%

Plant Power: Opportunities and challenges for meeting sustainable energy needs from the plant and fungal kingdoms

Grace

Lovett

Gore

et al. 2020

Plants People Planet

View full text Add to dashboard Cite

show abstract

“…Fungal groups that can be found in fresh cattle manure are the m o n o p h y l e t i c g r o u p o f s t r i c t l y a n a e r o b i c Neocallimastigomycota that greatly promotes degradation of lignocellulose-rich biomass in their hosts (Griffith et al 2010). Their survival under aerobic conditions is however very limited (Leis et al 2014;Nagler et al 2019). Thus, it is unlikely that the detected fungal sequences at later time points were affiliated with Neocallimastigomycota.…”

Section: Microbial Community Evolutionmentioning

confidence: 99%

Robustness of the autochthonous microbial soil community after amendment of cattle manure or its digestate

et al. 2019

Self Cite

View full text Add to dashboard Cite

In the last two decades, a change in land use has taken place in Europe. Manures that used to be applied to agricultural soils are now used in biogas reactors, and instead, digestate is applied. Here, we simulated soil amendment with either fresh or anaerobically digested cattle manure. The aim was to investigate the resilience of the resident microbiota and detect differences in the microbial biomass and activity after fertilizer amendment. Furthermore, the physiological community profile and the role of the indigenous microbial community was elucidated. In a microcosm experiment, two kinds of agricultural soil (γ-irradiated versus non-irradiated) were amended with either treatment. The effect of amendments on the community composition and physiological activity was tested immediately, after 1 and 3 months of incubation through 16S rRNA gene amplicon sequencing (initial soil community), genetic and physiological profiling (PCR-DGGE and MicroResp™), and measurement of basal respiration and microbial biomass. Either fertilizer did not affect the community composition of dominant fungi and bacteria in non-irradiated soils. This indicates the ability of the indigenous microbiota to outcompete allochthonous microorganisms. Soil microbial biomass was not changed, whereas basal respiration was significantly higher after amendment, especially when using fresh manure. MicroResp™ revealed slightly higher respiration for some substrates after 1 month; this finding was, however, not persistent and similar for manure and digestate. Generally, after 1 month, treatments returned to control levels for all parameters.In conclusion, amendment with anaerobically digested manure did not have a greater impact on soil microbial properties.

show abstract

Simple yet effective: Microbial and biotechnological benefits of rumen liquid addition to lignocellulose-degrading biogas plants

Cited by 32 publications

References 33 publications

Anaerobic Fungi: Past, Present, and Future

Anaerobic Fungi: Past, Present, and Future

Plant Power: Opportunities and challenges for meeting sustainable energy needs from the plant and fungal kingdoms

Robustness of the autochthonous microbial soil community after amendment of cattle manure or its digestate

Contact Info

Product

Resources

About