Simple Synthesis of Hierarchically Ordered Mesocellular Mesoporous Silica Materials Hosting Crosslinked Enzyme Aggregates

Lee, Jun Young; Kim, Jungbae; Kim, Jaeyun; Hong, Jin; Kim, Moon Il; Kwak, Ja Hun; Jin, Sun; Dohnálková, Alice; Park, Hyun Gyu; Chang, Ho Nam; Wang, Ping; Grate, Jay W.; Hyeon, Taeghwan

doi:10.1002/smll.200500035

Cited by 194 publications

(152 citation statements)

References 81 publications

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“…Enzyme biosensors are made by immobilization of enzymes on the sensing surface [98][99][100]. Due to the presence of reactive functional groups, organic polymeric carriers such as poly(2-glucosyloxyethyl methacrylate)-concanavalin [101], polyethylenimine [102], and polyvinylferrocenium [103] are used for this purpose.…”

Section: Biosensorsmentioning

confidence: 99%

Enzyme immobilization: an update

et al. 2013

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Compared to free enzymes in solution, immobilized enzymes are more robust and more resistant to environmental changes. More importantly, the heterogeneity of the immobilized enzyme systems allows an easy recovery of both enzymes and products, multiple re-use of enzymes, continuous operation of enzymatic processes, rapid termination of reactions, and greater variety of bioreactor designs. This paper is a review of the recent literatures on enzyme immobilization by various techniques, the need for immobilization and different applications in industry, covering the last two decades. The most recent papers, patents, and reviews on immobilization strategies and application are reviewed.

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Section: Biosensorsmentioning

confidence: 99%

Enzyme immobilization: an update

et al. 2013

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“…For mesoporous silica particles, the approach of crosslinked enzyme aggregates was reported as a simple and effective method for enzyme stabilization (51,52). Crosslinked enzyme aggregates were prepared by crosslinking enzyme molecules after adsorption into the channel of mesoporous silica particles (53).…”

Section: Crosslinked Enzyme Aggregatesmentioning

confidence: 99%

Inorganic nanomaterial-based biocatalysts

Lee

Lee²,

Chang³

et al. 2011

BMB Reports

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Over the years, nanostructures have been developed to enable to support enzyme usability to obtain highly selective and efficient biocatalysts for catalyzing processes under various conditions. This review summarizes recent developments in the nanostructures for enzyme supporters, typically those formed with various inorganic materials. To improve enzyme attachment, the surface of nanomaterials is properly modified to express specific functional groups. Various materials and nanostructures can be applied to improve both enzyme activity and stability. The merits of the incorporation of enzymes in inorganic nanomaterials and unprecedented opportunities for enhanced enzyme properties are discussed.

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“…Stabilization of one enzyme or multiple enzymes on solid supporters is a well-known technology. With the rapid development of nanomaterials with much larger surface areas (i.e., more enzymes can be immobilized), ultra-stable immobilized enzymes have been reported to be active for several months or years (Bornscheuer, 2003;Lee et al, 2005Lee et al, , 2007Polizzi et al, 2007;St. Clair et al, 2000;Vasic-Racki, 2006).…”

Section: Cost Analysis and Perspectivesmentioning

confidence: 99%

Production of biocommodities and bioelectricity by cell‐free synthetic enzymatic pathway biotransformations: Challenges and opportunities

Zhang

2010

Biotech & Bioengineering

163

137

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Cell-free synthetic (enzymatic) pathway biotransformation (SyPaB) is the assembly of a number of purified enzymes (usually more than 10) and coenzymes for the production of desired products through complicated biochemical reaction networks that a single enzyme cannot do. Cell-free SyPaB, as compared to microbial fermentation, has several distinctive advantages, such as high product yield, great engineering flexibility, high product titer, and fast reaction rate. Biocommodities (e.g., ethanol, hydrogen, and butanol) are low-value products where costs of feedstock carbohydrates often account for $30-70% of the prices of the products. Therefore, yield of biocommodities is the most important cost factor, and the lowest yields of profitable biofuels are estimated to be ca. 70% of the theoretical yields of sugar-to-biofuels based on sugar prices of ca. US$ 0.18 per kg. The opinion that SyPaB is too costly for producing low-value biocommodities are mainly attributed to the lack of stable standardized building blocks (e.g., enzymes or their complexes), costly labile coenzymes, and replenishment of enzymes and coenzymes. In this perspective, I propose design principles for SyPaB, present several SyPaB examples for generating hydrogen, alcohols, and electricity, and analyze the advantages and limitations of SyPaB. The economical analyses clearly suggest that developments in stable enzymes or their complexes as standardized parts, efficient coenzyme recycling, and use of low-cost and more stable biomimetic coenzyme analogs, would result in much lower production costs than do microbial fermentations because the stabilized enzymes have more than 3 orders of magnitude higher weight-based total turn-over numbers than microbial biocatalysts, although extra costs for enzyme purification and stabilization are spent.

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Simple Synthesis of Hierarchically Ordered Mesocellular Mesoporous Silica Materials Hosting Crosslinked Enzyme Aggregates

Cited by 194 publications

References 81 publications

Enzyme immobilization: an update

Enzyme immobilization: an update

Inorganic nanomaterial-based biocatalysts

Production of biocommodities and bioelectricity by cell‐free synthetic enzymatic pathway biotransformations: Challenges and opportunities

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