Simple synthesis of cytidine diphosphate choline

Improved Synthesis of Cytidine Diphosphate Choline (CDP-Choline) Via Selective Phosphorylation

“…4 Among various methods for the synthesis of CDP-choline in the literature, the current preferred method is via the condensation of cytidine-5'-phosphomorpholide (2) with choline phosphate chloride (3) under mild reaction conditions. [5][6][7] Compound 2 was synthesised from 5'-cytidine monophosphate (4) and morpholine in the presence of DCC (N,N'-dicyclohexylcarbodiimide) 8 or via the controlled hydrolysis of cytidine-5'-phosphodichloride (5) followed by P-N bond formation with morpholine (Scheme 1, route a). 7 However, DCC is toxic and converted into urea which is difficult to separate from the mixture, thus leading to poor purity of product.…”