Simple staining procedure permits rapid counting of mycoplasma colonies

Cluss, R G; Somerson, Norman L.

doi:10.1128/jcm.19.4.543-545.1984

Cited by 4 publications

(3 citation statements)

References 13 publications

(8 reference statements)

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“…These isolates produce characteristic ‘fried egg’ colonies after passage (OIE 2017). Identification of isolated mycoplasma in the initial stage is done by conventional (Gram staining) and specific staining (Diene’s stain) or by serological tests, growth inhibition (GI) test or biochemical tests (Cluss and Somerson 1984; Sadique et al. 2012c; Shah et al.…”

Section: Diagnosismentioning

confidence: 99%

Contagious caprine pleuropneumonia – a comprehensive review

Yatoo

Parray

Bashir

et al. 2019

Veterinary Quarterly

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Contagious caprine pleuropneumonia (CCPP) is a serious disease of goats, occasionally sheep and wild ruminants, caused by Mycoplasma capricolum subspecies capripneumoniae (Mccp). The disease is characterized by severe serofibrinous pleuropneumonia, very high morbidity ($100%), and mortality (80-100%). CCPP affects goats in more than 40 countries of the world thereby posing a serious threat to goat farming around the globe. The characteristic clinical signs of CCPP are severe respiratory distress associated with sero-mucoid nasal discharge, coughing, dyspnea, pyrexia, pleurodynia, and general malaise. In later stages, severe lobar fibrinous pleuropneumonia, profuse fluid accumulation in pleural cavity, severe congestion of lungs and adhesion formation is observed. Mycoplasmal antigen interactions with host immune system and its role in CCPP pathogenesis are not clearly understood. CCPP is not a zoonotic disease. Diagnosis has overcome cumbersome and lengthy conventional tests involving culture, isolation, and identification by advanced serological (LAT, cELISA) or gene-based amplification of DNA (PCR, RFLP, and hybridization) and sequencing. The latex agglutination test (LAT) is rapid, simple, and better test for field and real-time diagnosis applicable to whole blood or serum and is more sensitive than the CFT and easier than the cELISA. Moreover, the studies on antibiotic sensitivity and exploration of novel antibiotics (fluoroquinolones, macrolides) can help in better therapeutic management besides preventing menace of antibiotic resistance. Re-visiting conventional prophylactic measures focussing on developing novel strain-based or recombinant vaccines using specific antigens (capsular or cellular) should be the most important strategy for controlling the disease worldwide.

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Section: Diagnosismentioning

confidence: 99%

Contagious caprine pleuropneumonia – a comprehensive review

Yatoo

Parray

Bashir

et al. 2019

Veterinary Quarterly

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“…All platings were performed in triplicate. Colonies were stained with the lipid-protein stain (6) and then were counted with the aid of a bacterial colony counter.…”

Section: Methodsmentioning

confidence: 99%

Interaction of albumin and phospholipid:cholesterol liposomes in growth of Mycoplasma spp

Cluss¹,

Somerson²

1986

Appl Environ Microbiol

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Mycoplasma spp., sterol and fatty acid auxotrophs, are conventionally grown in complex media containing high concentrations of serum. Serum supplies the required lipids, but its presence complicates studies on the metabolism and antigenicity of mycoplasmas as well as the membrane dynamics of these organisms. In the present work, fetal bovine serum was replaced with dilipidated albumin and liposomes containing high concentrations of cholesterol. The liposomes were produced from phosphatidylcholine which contained other lipid species, including phosphatidylethanolamine, phosphatidylglycerol, and cholesterol. Other liposomes containing cholesterol and one phospholipid yielded significantly less growth of Mycoplasma gallisepticum, indicating that several phospholipids are required to achieve growth levels comparable to those obtained with complex medium. The sources and concentrations of cholesterol, albumin, phosphatidylcholine, and other phospholipids and the interactions among them were important affectors of mycoplasmal growth. Optimal lipid and albumin conditions established for M. gaUlisepticum were then used to propagate five diverse Mycoplasma spp. to growth levels which equalled or surpassed those obtained with medium containing 17% fetal bovine serum.

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“…Bacterial staining, usually Gram staining, is often used to guide therapy in patients with infections [9], and particularly considering increased antibiotic resistance [10]. Other stainings, such as simple staining and vital staining, may be used to rapidly detect the presence of bacteria or even pathogenic bacteria using a single stain method [7,11,12].…”

Section: Introduction mentioning

confidence: 99%

The Use of Food Coloring Dyes in Bacterial Staining

Risandiansyah

Arniyati

Nurita

et al. 2020

JELS

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Staining creates a contrast between the cells and its surrounding, and enables the microscopic characteristics of bacterial cells to be easily visible and distinguished. However, staining often relies on dyes which are expensive, not readily available, or toxic. In this study, the use of food coloring dyes to stain bacteria was explored. We stained Grampositive bacteria (Bacillus sp. and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) using several food coloring dyes of different colors, which were purchased locally. After slide fixation, the dye was flooded on the bacterial smear and air-dried for up to 30 minutes and observed by using microscope before and after washing with water. The results of this study show that prior to washing, most food coloring dyes were able to stain bacterial cells. However, after washing, only pink and purple food coloring dyes were retained, showing pink colored cells. We suspected that erythrosine was the agent responsible for this result, and was able to show similar characteristics with erythrosine alone. This study concludes that food coloring dyes containing erythrosine can be used to stain bacterial cells indiscriminately.

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Simple staining procedure permits rapid counting of mycoplasma colonies

Abstract: A staining procedure employing oil red 0 and Coomassie R-250 was developed to increase visualization of mycoplasma colonies. This procedure permits CFU determination of mycoplasmas without additional microscopy.

Cited by 4 publications

References 13 publications

Contagious caprine pleuropneumonia – a comprehensive review

Contagious caprine pleuropneumonia – a comprehensive review

Interaction of albumin and phospholipid:cholesterol liposomes in growth of Mycoplasma spp

The Use of Food Coloring Dyes in Bacterial Staining

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