Simple solid-phase extraction method for determination of polychlorinated biphenyls and selected organochlorine pesticides in human serum

Čonka, Kamil; Drobná, Beata; Kočan, Anton; Petrı́k, Ján

doi:10.1016/j.chroma.2004.11.029

Cited by 86 publications

(48 citation statements)

References 8 publications

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“…In summary, the procedure included standardized extraction, clean-up and quantification accompanied by a quality control program (Conka et al, 2005). Serum spiked with a known amount of PCB-174 congener, which is relatively low in the Slovak population and hence served as a recovery standard, was treated with a mixture of water and 1-propanol to denaturate the proteins.…”

Section: Pcb Measurementmentioning

confidence: 99%

Serum PCB concentrations in relation to locally produced food items in eastern Slovakia

Sonneborn

Park

Babinská

et al. 2008

J Expo Sci Environ Epidemiol

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Ingestion has been a primary route of PCB exposure for people, especially those not working directly in industrial settings. During 2002During -2004 were recruited at delivery from two districts in eastern Slovakia: Michalovce with high PCB contamination from a chemical manufacturing plant, and Svidnik located 70 km to the northwest, having lower environmental levels of PCBs. Concentrations of 15 PCB congeners were measured in maternal serum using high-resolution gas chromatography with electron capture detection. A food frequency questionnaire was developed and validated at the Research Institute of Nutrition and School of Medicine in Bratislava, Slovakia. The questionnaire was comprised of 88 food items representative of the national dietary habits and designed to ascertain (1) the source of food items as reported by the women (locally produced or purchased from a retail outlet) and (2) quantities consumed of high-fat food categories representative of the national dietary habits. Our primary goal was to identify specific food sources, either locally produced or purchased from retail stores, that might predict serum concentrations of PCBs. We used multiple linear regression to examine the relationship of dietary fats to lipid-adjusted serum PCB levels in 948 adult women (Michalovce N ¼ 662, Svidnik N ¼ 284) who had recently given birth. We adjusted for residential district, age, body mass index, education and duration of previous lactation. Consumption of fat from locally produced foods was significantly associated with higher levels of lipid-adjusted serum PCB (b ¼ 0.06, P ¼ 0.007). Fat from foods purchased in retail outlets showed no significant association (b ¼ À0.02, P ¼ 0.36). There was no interaction between district and diet in predicting serum PCB levels. Comparing women in Michalovce consuming 20 g of fat per day from local sources with those consuming 1 g of fat per day from local sources, lipidadjusted serum concentrations were predicted to be higher by 81 ng/g lipid or 14.8% 630 ng/g lipid versus 549 ng/g lipid.

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Section: Pcb Measurementmentioning

confidence: 99%

Serum PCB concentrations in relation to locally produced food items in eastern Slovakia

Sonneborn

Park

Babinská

et al. 2008

J Expo Sci Environ Epidemiol

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“…Unmodified silica gel, aluminum oxide and Florisil, usually called normal phase materials, separate sample constituents into fractions of comparable polarity. They are often utilized to separate and concentrate pesticides, [207] PCBs, [208] polychlorodibenzodioxins and dibenzofurans (PCDD/PCDFs) [209] and for the simultaneous determination of bisphenol A, triclosan and tetrabromobisphenol A [210] from biological, agricultural and environmental samples.…”

Section: Solid Phase Extraction (Spe)mentioning

confidence: 99%

Sample Preparation for Trace Analysis by Chromatographic Methods

Olariu

Vione

Grinberg

et al. 2010

Journal of Liquid Chromatography & Related Technologies

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The determination of trace analytes in complex natural matrices often requires extensive sample extraction and preparation prior to chromatographic analysis. Correct sample preparation can reduce analysis time, sources of error, enhance sensitivity and enable unequivocal identification, confirmation and quantification. This overview considers general aspects on sample preparation techniques for trace analysis in various matrices. The discussed extraction/enrichment techniques cover classical methods, such as Soxhlet and liquid-liquid extractions along with more recently developed techniques like pressurized liquid extraction, liquid phase microextraction (LPME), accelerated microwave extraction, and ultrasoundassisted extraction. This overview also deals with more selective methodologies, such as solid phase extraction (SPE), solid phase microextraction (SPME) and stir bar sorptive extraction (SBSE). The adopted approach considers the equilibriums involved in each technique. The applicability of each technique in environmental, food, biological and pharmaceutical analyses is discussed, particularly for the determination of trace organic compounds by chromatographic methods.

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“…The literature describes several preparation methods to measure organochlorine pesticides in human serum Sandau et al, 2003;Focant et al, 2004;Conka et al, 2005;Ramos et al, 2007). The standard method consists of three steps: denaturation, extraction, and cleanup.…”

Section: Sample Preparation Of Serum For Analysismentioning

confidence: 99%