Simple sequence repeat (SSR) analysis for assessment of genetic variability in apricot germplasm

Abstract

Thirty SSR primer combinations, developed from peach SSR-enriched genomic libraries and BAC libraries of peach [ Prunus persica (L.) Batsch.], were tested for cross amplification with 74 apricot ( Prunus armeniaca L.) germplasm accessions. Twelve primer pairs amplified 14 polymorphic SSR loci useful for discriminating most apricot cultivars, as well as for investigating patterns of variation in apricot germplasm. Levels of polymorphism were higher than the levels described using other codominant marker systems (i.e., isozymes, RFLP markers). Overall, 107 alleles were identified, and all but 11 accessions were unambiguously discriminated. Genetic differentiation of native germplasm into traditional ecogeographical groups was low, with a high level of genetic identity (> 0.75) between the groups. However, neighbor joining cluster analysis of marker distances between cultivars reflected the complex history of apricot domestication, producing groupings not evidently based on the geographical origin of the cultivars. Distant positioning of Chinese cultivars on UPGMA and neighbor joining dendrograms supports the authors' consideration of Chinese apricots as subspecies, Prunus armeniaca var. ansu Maxim., rather than a separate species.

An analysis of apricot cultivars by random amplified polymorphic DNA and microsatellite primers

“…2, part 4b). In contrast with this result, Zhebentyayeva et al (2003) suggested In Bej Sin was localized with Moi Chua Sin. Genotypes Chuan Zhi Hong, Liaonig and Čína 2-189 are common for part 4b in the RAPD dendrogram (Fig.…”

“…Forum is formed by crossing cultivars of European and Irano-Caucasian origin. Zhebentyayeva et al (2003) confirmed that Vynoslivyj was localized inside the group of European varieties.…”

“…2). In the same way Zhebentyayeva et al (2003) revealed that the Shalah cultivar and similar Arzami cultivar are clustered together. Both cultivars are similar to Chinese apricot cultivars in anatomical and morphological traits (Kovaljov 1963;Rostova, Sokolova 1992).…”

“…In the same way Zhebentyayeva et al (2003) detected that the Velkopavlovická cultivar was grouped as part of the European varieties. Maghuly et al (2006) supposed that Velkopavlovická was clustered with Ksna Ugarska, near the group of European cultivars.…”

“…2, part 4a, the Churmai and Mulla Sadik cultivars come from Central Asia. Zhebentyayeva et al (2003) analysed a similar cultivar Churmai Ranni, which clustered with Kok-pshar and Samyi Rannii cultivars. Genotypes Churmai and Mulla Sadik are common for part 4a in the RAPD (Fig.…”