2005
DOI: 10.1007/s00122-004-1814-6
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Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.)

Abstract: A set of 118 simple sequence repeat (SSR) markers has been developed in melon from two different sources: genomic libraries (gSSR) and expressed sequence-tag (EST) databases (EST-SSR). Forty-nine percent of the markers showed polymorphism between the 'Piel de Sapo' (PS) and PI161375 melon genotypes used as parents for the mapping populations. Similar polymorphism levels were found in gSSR (51.2%) and EST-SSR (45.5%). Two populations, F2 and a set of double haploid lines (DHLs), developed from the same parent g… Show more

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Cited by 165 publications
(122 citation statements)
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References 47 publications
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“…Among the mapped loci, only one (AFLP) marker presented segregation distortion. 12 LG were named in accordance to Périn et al (2002), based on SSR markers mapped by Gonzalo et al (2005) and Fukino et al (2008 Figure 1. Linkage map of F 2 population (PI 414723 × Védrantais).…”
Section: Linkage Map and Gene Mappingmentioning
confidence: 99%
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“…Among the mapped loci, only one (AFLP) marker presented segregation distortion. 12 LG were named in accordance to Périn et al (2002), based on SSR markers mapped by Gonzalo et al (2005) and Fukino et al (2008 Figure 1. Linkage map of F 2 population (PI 414723 × Védrantais).…”
Section: Linkage Map and Gene Mappingmentioning
confidence: 99%
“…Forty seven microsatellite markers polymorphic between PI 414723 and Védrantais were selected from the linkage maps described by Fukino et al (2008) and Gonzalo et al (2005), in order to represent all linkage groups and provide a map framework. The PCR reactions consisted of 20 ng of DNA, 0.6 µM of each primer, 1X PCR Master Mix (Promega -M7505) and ultrapure sterile water to complete 12 µl.…”
Section: Molecular Markersmentioning
confidence: 99%
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“…Their nomenclature assignment were carried out following the indications suggested by the different authors, being named as CM-and CS- (Daning-Poleg et al 2001), CMBR (Ritschel et al 2004), CM-N and TJ (Gonzalo et al 2005), and CMN (Fukino et al 2007). Reverse primers of each SSR primer pairs were fluorescently labeled with WellRED fluorescent dyes D2, D3 or D4 (Proligo, Paris, France), and PCR amplifications were carried out using a thermal cycler (cycler, Bio-Rad Laboratories, Hercules, CA, USA).…”
Section: Molecular Evaluationmentioning
confidence: 99%
“…Recently, Feng et al (2009) developed 87 EST-SSR markers from NCBI database. Because of the conservative sequences of ESTs, The level of polymorphism in EST-SSR was lower compared to that of genomic-SSR marker (Eujayl et al, 2002;Leigh et al, 2003;Gonzalo et al, 2005;Yang et al, 2005;Pinto et al, 2006). In this study, gSSRs produced more polymorphisms than EST-SSR as well as PD was slightly higher, despite HBE280 was the most informative marker which belonged to EST-SSRs.…”
Section: The Polymorphism Of Est-and Gssrmentioning
confidence: 48%