Simple sequence repeat markers linked to a major QTL controlling pod shattering in soybean

Abstract

Shattering of soybean pods prior to harvest leads to a reduction in yield. In order to identify simple sequence repeat (SSR) markers linked to quantitative trait loci (QTLs) conditioning pod shattering, QTL analysis was conducted using an recombinant inbred line (RIL) population segregating for this trait. The degrees of pod-shattering resistance were evaluated by heat treatment applied to pods harvested from plants in the field and in a growth chamber. Composite interval mapping identified one major QTL between SSR markers Sat_093 and Sat_366 on linkage group J for both environments. The position and the effect of this QTL were confirmed in an F 2 population derived from a cross between the pod shattering-susceptible parental cultivar and a pod shattering-resistant RIL. The SSR markers linked to the major QTL will be useful for marker-assisted selection in soybean-breeding programmes.

A Major Soybean QTL,qPDH1, Controls Pod Dehiscence without Marked Morphological Change

“…The effect of qPDH1 was demonstrated to be so large that this QTL accounted for more than 50% of total variance for pod dehiscence percentage in the populations of recombinant inbred lines (RILs) and F 2 plants (Funatsuki et al, 2006). A QTL that is presumed to be identical to qPDH1 also explained nearly 50% of total variance in another RIL population (Bailey et al, 1997).…”

“…To evaluate shattering resistance, we used heat treatment as in previous studies (Tsuchiya, 1986;Jiang et al, 1991;Tukamuhabwa et al, 2002;Funatsuki et al, 2006;Romkaew and Umezaki, 2006). The pods used for the measurement of whole pod morphology were also used for evaluation of shattering resistance.…”

“…To detect quantitative trait loci (QTLs) and the linked molecular markers, we conducted QTL analysis using the progeny derived from a cross between a shatteringresistant cultivar, Hayahikari, and a susceptible one, Toyomusume (Funatsuki et al, 2006). A major QTL, qPDH1, was identified, and along with the linked markers it was suggested to be useful for Japanese soybean breeding (Funatsuki et al, 2008).…”

Pod Shattering: A Homologous Series of Variation Underlying Domestication and an Avenue for Crop Improvement

“…Using recombinant inbred lines and F2 populations, a major QTL controlling pod shattering in soybean was found [93,95,97,98]. The major QTLs mapped in these studies all mapped closely to each other, suggesting they are the same locus.…”

“…Genetic analysis of pod shattering has been carried out in various legumes including soybean [48,[93][94][95][96][97][98][99][100][101], common bean [13,44], pea [18,50,102], cowpea [49,[103][104][105][106], lentil [16,46,107], narrow-leaf lupin [47,108], azuki bean [45,109], and common vetch [43,110]. In most of the legumes that have been studied so far, pod shattering is found to be a dominant trait controlled by one or two genes or QTLs (Table 1).…”