Simple, sensitive and specific bioassay of interleukin-1

Hopkins, Stephen J.; Humphreys, M.

doi:10.1016/0022-1759(89)90252-4

Cited by 148 publications

(66 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IL-1 was determined in a proliferation assay using D10(N4)M cells [14,15]. One unit of IL-1 activity was defined as the reciprocal of the dilution producing 50% proliferation of D10(N4)M cells.…”

Section: Cytokine Bioassays and Elisamentioning

confidence: 99%

“…One day after seeding, blood monocytes and alveolar macrophages were treated with recombinant porcine IL-1b (gift from A. Billiau, Leuven, Belgium) at a concentration of 200 U/mL (75 ´ 10 3 ng/mL) or with recombinant porcine IL-10 (Biosource Europe, Nivelles, Belgium) at concentrations of 1, 10, or 100 ng/mL. Functional activity of both recombinant porcine IL-1b and recombinant porcine IL-10 had been proven in a proliferation assay using D10(N4)M cells [14,15] and the mouse mast cell line D36 [23], respectively. Untreated cells were included as controls.…”

Section: In Vitro Experimentsmentioning

confidence: 99%

See 1 more Smart Citation

Apoptosis in the lungs of pigs infected with porcine reproductive and respiratory syndrome virus and associations with the production of apoptogenic cytokines

et al. 2003

View full text Add to dashboard Cite

-Apoptosis was studied in the lungs of pigs during an infection with a European strain of porcine reproductive and respiratory syndrome virus (PRRSV) and it was examined if cytokines were involved in the induction of apoptosis. Twenty-two 4-to 5-week-old gnotobiotic pigs were inoculated intranasally with 10 6.0 TCID 50 of the Lelystad virus and euthanised between 1 and 52 days post inoculation (PI). The lungs and broncho-alveolar lavage (BAL) cells were assessed both for virus replication and apoptosis; BAL fluids were examined for interleukin (IL)-1, tumour necrosis factor-alpha and IL-10. Double-labellings were conducted to determine the relation between virus replication and apoptosis and to identify the apoptotic cells. Apoptosis occurred in both infected and non-infected cells. The percentages of infected cells, which were apoptotic, ranged between 9 and 39% in the lungs and between 13 and 30% in the BAL cells. The majority of apoptotic cells were non-infected. Non-infected apoptotic cells in the lungs were predominantly monocytes/macrophages, whereas those in the broncho-alveolar spaces were predominantly lymphocytes. The peak of apoptosis in the lungs at 14 days PI was preceded by a peak of IL-1 and IL-10 production at 9 days PI, suggesting a possible role of these cytokines in the induction of apoptosis in non-infected interstitial monocytes/macrophages. However, the latter hypothesis was not confirmed in vitro, since blood monocytes or alveolar macrophages did not undergo apoptosis after treatment with recombinant porcine IL-1 or IL-10. pig / lung / porcine reproductive and respiratory syndrome virus / apoptosis / cytokine

show abstract

Section: Cytokine Bioassays and Elisamentioning

confidence: 99%

Section: In Vitro Experimentsmentioning

confidence: 99%

Apoptosis in the lungs of pigs infected with porcine reproductive and respiratory syndrome virus and associations with the production of apoptogenic cytokines

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Biologically active IL-Iß was determined using growth factor-dependent D10(N4)M cells [37]. Cells were maintained in RPMI 1640 medium supplemented with 10% FCS, 2 mM L-glutamine, 100 IU/ml penicillin G, 100 u.g/ml streptomycin, 1 mM sodium pyruvate, 5 mM ß-mercaptoethanol and 10% supernatant of phorbol ester-stimulated EL-4 cells as a source of IL-2, and 10% supernatant of phorbol esterstimulated P388D1 cells as a source of IL-1.…”

Section: Pil-lß-processing Assaymentioning

confidence: 99%

“…MCF7, COS7, HeLa and L929s. Secreted mature IL-lß was quantified in a bioassay using DIO cells [37] (Fig. 5).…”

Section: Pil-lfi Is Mainly Processed By Mcasp-1 But Also Bymentioning

confidence: 99%

Characterization of seven murine caspase family members

et al. 1997

View full text Add to dashboard Cite

Seven members of the murine caspase (mCASP) family were cloned and functionally characterized by transient overexpression: mCASP-1 (mICE), mCASP-2 (Ichl), mCASP-3 (CPP32), mCASP-6 (Mch2), mCASP-7 (Mch3), mCASP-11 (TX) and mCASP-12. mCASP-11 is presumably the murine homolog of human CASP-4. Although mCASP-12 is related to human CASP-5 (ICE re i-III), it is most probably a new CASP-1 family member. On the basis of sequence homology, the caspases can be divided into three subfamilies: first, mCASP-1, mCASP-11 and mCASP-12; second, mCASP-2; third, mCASP-3, mCASP-6 and mCASP-7. The tissue distribution of the CASP-1 subfamily transcripts is more restricted than that of the CASP-3 subfamily transcripts, suggesting that the transcriptional regulation of the CASP members within one subfamily is related, but is quite different between the CASP-1 and the CASP-3 subfamilies. Transient overexpression of each of the seven CASPs induced apoptosis in mammalian cells. Only two, mCASP-1 as well as mCASP-3, were able to process precursor interleukin (IL)-lß to biologically active IL-lß. In addition, mCASP-3 is the predominant PARP-cleaving enzyme in vivo.

show abstract

“…Correspondingly the coexistence of 2 different IL-1 receptors on such cells has been implicated [6] and corroborated by differential blockade of IL-1 responses by M15, a monoclonal antibody directed against the 80 kDa type I IL-1 receptor [7]. In fact, an IL-1 receptor with a lower molecular weight apart from the 80 kDa IL-1 receptor has been detected in D10S cells [8] and D10.G4.1 cells [9] and according to peptide mapping analysis it was suggested to be a related, probably processed form of Here we have analyzed the IL-1 receptor heterogeneity of D10N cells, another subline of D10.G4.1 [12] by cross-linking experiments, complemented with inhibition studies using the IL-1 receptor antagonist (IL-lra). Further, we provide proof of identity of two IL-1 receptors in D10N cells by PCR technique.…”

Section: Introductionmentioning

confidence: 95%

Coexpression of type I and type II IL‐1 receptors in the murine T helper 2 cell line D10N

Brigelius‐Flohé

McCarthy²,

Resch

et al. 1993

FEBS Letters

View full text Add to dashboard Cite

I L-I receptor heterogeneity in murine lymphocytes was investigated by cross-linking to [~25I]IL-1~ and competition with IL-1 receptor antagonist, and the molecular identity of the IL-1 receptors was identified with PCR using primers specific for type I and type II IL-I receptors. The thymoma cell line EI4 6.1 exhibits exclusively the 80 kDa receptors which proved to be the type I receptor according to PCR analysis. In the pre-B cell line 70Z/3, predominantly a 60 kDa type II receptor but also a trace of type I receptor can be identified by PCR. The Th2 cell line D10N expresses both types of IL-1 receptors in equivalent amounts according to cross-linking experiments and PCR. The proliferative response of D 10N cells to IL-1 is inhibited by IL-1 ra which according to cross-linking affects the binding to the type I receptor only. It is concluded that coexpression of both types of IL-1 receptors might be a characteristic of murine Th2 cells and that their growth-dependence on IL-I is mediated by the type I receptor.Interleukin-1 receptor; Interleukin-1; Interleukin-1 receptor antagonist; Mouse T helper cell

show abstract

Simple, sensitive and specific bioassay of interleukin-1

Cited by 148 publications

References 13 publications

Apoptosis in the lungs of pigs infected with porcine reproductive and respiratory syndrome virus and associations with the production of apoptogenic cytokines

Apoptosis in the lungs of pigs infected with porcine reproductive and respiratory syndrome virus and associations with the production of apoptogenic cytokines

Characterization of seven murine caspase family members

Coexpression of type I and type II IL‐1 receptors in the murine T helper 2 cell line D10N

Contact Info

Product

Resources

About