Simple repetitive sequences are associated with differentiation of the sex chromosomes in the guppy fish

Nanda, Indrajit; Feichtinger, W.; Schmid, Michael; Schröder, Johannes Horst; Zischler, Hans; Epplen, Jörg T.

doi:10.1007/bf02101117

Cited by 126 publications

(95 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In some inbred lines of the guppy male specific hybridization in the high molecular weight region is absent, which may be related to the Y chromosome polymorphism in natural populations (Nanda et al 1990). (2) In those systems where genetic experiments on the sex chromosome mechanisms have not been reported, the sex chromosome status could be inferred by specific hybridization pattems (P. velifera and P. sphenops).…”

Section: Discussionmentioning

confidence: 99%

“…The techniques used for the preparation of mitotic chromosomes, demonstration of constitutive heterochromatin and in situ hybridization using the biotinylated (GACA) 4 oligonucleotide have been described previously (Haaf and Schmid 1984;Nanda et al 1990). …”

Section: Methodsmentioning

confidence: 99%

“…A sirnilar slight difference in the Cbanding pattems has been observed between the telomeres of the homologous No. 1 chromosomes in the male guppy (Nanda et al 1990). In one of the homologues (Y) there is always distinctly more telomeric heterochromatin than in the other (X).…”

Section: Gaca Ggatmentioning

confidence: 99%

“…Hybridization of (GACA) 4 to the chromosomes displays the characteristic peroxidase reaction at the telomeric end of only one of the No. 1 homologues in males (Nanda et al 1990). Thus the male-specific hybridization pattern with the (GACA) 4 probe is due to the accumulation of simple repeats on the telomeric heterochromatin .…”

Section: Gaca Ggatmentioning

confidence: 99%

“…A considerable part of this satellite sequence consists of particular simple repetitive sequences, (gata)n and (gaca)m, which are present in a wide range of vertebrates (Epplen 1988). These simple repeats have allowed the detection of molecular and structural differences even between apparently homomorphic sex chromosomes in the guppy (Nanda et al 1990). Here we present studies on Xiphophorus and Poecilia species undertaken in order (1) to analyse the association of simple repetitive sequences with the genetically apparent heterogamety and (2) to confirm the presence of simple repetitive sequences like (gata)n and (gaca)m in the early evolution of the heterogametic condition.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Early stages of sex chromosome differentiation in fish as analysed by simple repetitive DNA sequences

Nanda

Schartl²,

Feichtinger

et al. 1992

Chromosoma

Self Cite

View full text Add to dashboard Cite

Abstract. Animal sex chromosome evolution has started on different occasions with a homologous pair of autosomes leading to morphologically differentiated gonosomes. In contrast to other vertebrate classes, among fishes cytologically dernonstrahle sex chromosomes are rare. In reptiles, certain motifs of simple tandemly repeated DNA sequences like (gata)n/(gaca)m are associated with the constitutive heterochromatin of sex chromosomes. In this study a panel of simple repetitive sequence probes was hybridized to restriction enzyme digested genomic DNA of poeciliid fishes. Apparent male heterogamety previously established by genetic experiments in Poecilia reticulata (guppy) was correlated with male-specific hybridization using the (GACA) 4 probe. The (GAT A) 4 oligonucleotide identifies certain male guppies by a Y chromosomal polymorphism in the outbred population. In cantrast none of the genetically defined heterogametic situations in Xiphophorus could be verified consistently using the collection of simple repetitive sequence probes. Only individuals from particular populations produced sex-specific patterns of hybridization with (GAT A) 4 • Additional poeciliid species (P. sphenops, P. velifera) harbour different sex-specifically organized simple repeat motifs. The observed sex-specific hybridization patterns were substantiated by banding analyses of the karyotypes and by in situ hybridization using the (GACA) 4 probe.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Gaca Ggatmentioning

confidence: 99%

Section: Gaca Ggatmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Early stages of sex chromosome differentiation in fish as analysed by simple repetitive DNA sequences

Nanda

Schartl²,

Feichtinger

et al. 1992

Chromosoma

Self Cite

View full text Add to dashboard Cite

show abstract

Is the Y chromosome of Drosophila an evolved supernumerary chromosome?

et al. 1996

View full text Add to dashboard Cite

SummaryThe Y chromosomes of most Drosophila species are necessary for male fertility but they are not involved in sex determination. They have many puzzling properties that resemble the effects caused by B chromosomes. Classical genetic and molecular studies reveal substantial affinities between Y and B chromosomes and suggest that the Y chromosomes of Drosophila are not degenerated homologues of the X chromosomes, but rather that their Y chromosomes evolved Accepted 7 December 1995 as specialized supernumeraries similar to classical B chromosomes.

show abstract

Plant DNA fingerprinting with radioactive and digoxigenated oligonucleotide probes complementary to simple repetitive DNA sequences

Beyermann²,

et al. 1991

View full text Add to dashboard Cite

The existence of hypervariable DNA sequences in nuclear genomes, and the use of appropriate "fingerprinting" probes to detect them, has gained widespread scientific interest, and also led to multiple applications in diverse areas. Two years ago, the new technique of "DNA fingerprinting" was also introduced into the analysis and characterization of plant genomes, initially by using human or M13 minisatellites as probes. In the present article, we demonstrate the applicability for plant DNA fingerprinting of oligonucleotide probes specific for simple repetitive DNA sequences. We show that various levels of intra- and interspecific polymorphisms can be detected; the information to be gained depends on the optimal combination of probe and species. Variety-specific patterns were obtained in several cases. Some probes revealed variability between individuals. Somatic variability was not observed. Different DNA isolation and purification procedures were tested in order to introduce a fast and easy-to-perform isolation method suitable for a large variety of plant species. Nonradioactive fingerprinting was performed using digoxigenated oligonucleotides as probes. Banding patterns obtained with radioactive and digoxigenin-based labeling techniques proved to be of similar quality.

show abstract

Simple repetitive sequences are associated with differentiation of the sex chromosomes in the guppy fish

Cited by 126 publications

References 14 publications

Early stages of sex chromosome differentiation in fish as analysed by simple repetitive DNA sequences

Early stages of sex chromosome differentiation in fish as analysed by simple repetitive DNA sequences

Is the Y chromosome of Drosophila an evolved supernumerary chromosome?

Plant DNA fingerprinting with radioactive and digoxigenated oligonucleotide probes complementary to simple repetitive DNA sequences

Contact Info

Product

Resources

About