Simple Rapid Microtechnic for Serum Total Cholesterol

Zak, Bennie

doi:10.1093/ajcp/27.5_ts.583

Cited by 572 publications

(100 citation statements)

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“…Reducing sugars content was determined by following the method of Nelson (1944). The content of phospholipids was estimated by determining phosphorus content as described by Ames (1966), glycolipids by measuring glucose content according to Roughan and Batt (1968), free fatty acids by copper complex formation as described by Lowry and Tinsley (1976) and sterols by using ferric chloride according to Zak (1957), all spectrophotometrically. The content of total glycerides was calculated by the difference of total lipid classes from 100.…”

Section: Methodsmentioning

confidence: 99%

Positional effects on soybean seed composition during storage

et al. 2011

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This investigation was conducted in order to determine the degree of biochemical changes during natural ageing of soybean seeds borne on different positions on stem axis. Biochemical analysis of field grown soybean seeds revealed significant differences in their oil and protein contents as a function of nodal positions. Both oil and protein content decreased after 180 days of storage (DOS) at all the nodal positions. Proportions of membrane lipids in seeds were less in basal as compared to apical positions and their proportion in stored seeds also decreased with increasing storage periods. The content of starch, total soluble sugars and reducing sugars in seeds decreased during storage for 180 days but it didn't show positional variations in their contents. The extent of lipid peroxidation increased during storage was associated with the corresponding decrease in the activities of antioxidant enzymes viz catalase and peroxidase in seeds. Seeds at basal positions showed higher rate of lipid peroxidation and lower peroxidase activity as compared to apical positions. Results suggest that soybean seeds collected from basal positions showed higher deteriorative changes during storage in comparison to apical positions that might be related to higher lipid content in seeds from basal portion of soybean stem axis as compared to apical portion.

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Section: Methodsmentioning

confidence: 99%

Positional effects on soybean seed composition during storage

et al. 2011

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“…The concentrations of hepatic TG and TC were determined with a Triglyceride G test kit (Wako Pure Chemical Industries Ltd., Osaka, Japan) and the Zak-Henly method. 9,10) The concentration of fecal TL was determined by a conventional gravimetric analysis. Experiment 2.…”

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confidence: 99%

Effect of Caffeine on the Body Fat and Lipid Metabolism of Rats Fed on a High-Fat Diet

Kobayashi‐Hattori

Mogi

Matsumoto

et al. 2005

Bioscience, Biotechnology, and Biochemistry

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The intake of caffeine (CF) at 0.025, 0.05 or 0.1% for 21 days progressively reduced the body fat mass and body fat percentage in Sprague-Dawley (SD) rats fed on a high-fat diet with increasing administration level. Moreover, CF increased the serum concentrations of catecholamines and free fatty acids in SD rats orally administered with CF (5 mg/kg). These results suggest that the intake of CF reduced body fat by lipolysis via catecholamines. CF has potential as a functional food ingredient with an anti-obesity action.

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“…After separating and evaporating the lipid layer, the residue was weighed and subjected to the determination of total lipids. The redissolved samples in chloroform were also used for the determinations of triglycerides (10), free fatty acids (11), phospholipids (12,13) and cholesterol (14) After iodine sublimed, the area containing TG was scraped off and extracted twice with chloroform-methanol (2:1). The extract was filtered and evaporated to dryness in a counting vial.…”

Section: Methodsmentioning

confidence: 99%

Hepatic lipogenesis and mobilization of peripheral fats in the formation of alcoholic fatty liver.

et al. 1981

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Abstract-Biochemical mechanisms underlying the development of alcoholic fatty liver were investigated.Acute ethanol (EtOH) administration for 3 days by an inhalation method, and continuous EtOH treatments by feeding with liquid diet or drinking water containing EtOH induced a significant increase of hepatic triglycerides (TG). A small but significant increase of TG was also observed in the blood serum.Although hepatic acetyl CoA carboxylase activity, measured in the presence and absence of citrate, was not altered by either acute or chronic EtOH administrations, fatty acid synthetase and malic enzyme activities in the liver were increased by continuous EtOH administration, but not in the acutely EtOH-treated animals.

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Simple Rapid Microtechnic for Serum Total Cholesterol

Cited by 572 publications

References 0 publications

Positional effects on soybean seed composition during storage

Positional effects on soybean seed composition during storage

Effect of Caffeine on the Body Fat and Lipid Metabolism of Rats Fed on a High-Fat Diet

Hepatic lipogenesis and mobilization of peripheral fats in the formation of alcoholic fatty liver.

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