Simple, rapid and sensitive reversed-phase high-performance liquid chromatographic method for the determination of mefenamic acid in plasma

Sato, Juichi; Owada, Eiji; Ito, Keiji; Niida, Yuichi; Wakamatsu, Akio; Umetsu, Masao

doi:10.1016/s0378-4347(00)82730-9

Cited by 18 publications

(10 citation statements)

References 9 publications

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“…Their mode of interaction is as potent prostaglandin synthetase inhibitors [1][2][3][4][5]. Since their introduction many manuscripts have appeared that discuss various structural and physical properties of fenamates [6][7][8][9][10][11][12] as well as numerous individual analytical methods for quantifying them [1][2][3][4][13][14][15][16][17][18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Infrared studies of the polymorphic states of the fenamates

Gilpin

Zhou

2005

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

Infrared studies of the polymorphic states of the fenamates

Gilpin

Zhou

2005

Journal of Pharmaceutical and Biomedical Analysis

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“…So far no LC-MS/MS bioanalytical method has been reported, although many high-performance liquid chromatographic (HPLC) methods have been reported (Niopas and Mamzoridi, 1994;Rouini et al, 2004;Sato et al, 1989;Singh et al, 1991;Yamashita et al, 1991). Most of the HPLC methods have been reported for the simultaneous determination of mefenamic acid along with other NSAIDs (Niopas and Mamzoridi, 1994;Singh et al, 1991;Yamashita et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

A high performance liquid chromatography–tandem mass spectrometric method for the determination of mefenamic acid in human plasma: application to pharmacokinetic study

Mahadik

Dhaneshwar

Bhavsar³

2012

Biomedical Chromatography

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In the present study, the development and validation of an LC-MS/MS method for quantifying mefenamic acid in human plasma is described. The method involves liquid-liquid extraction using diclofenac as an internal standard (IS). Chromatographic separation was achieved on a Thermo Hypurity C(18) , 50 × 4.6 mm, 5 µm column with a mobile phase consisting of 2 m m ammonium acetate buffer and methanol (pH 4.5 adjusted with glacial acetic acid; 15:85, v/v) at a flow-rate of 0.75 mL/min and the total run time was 1.75 min. Analyte was introduced to the LC-MS/MS using an atmospheric pressure ionization source. Both the drug and IS were detected in negative-ion mode using multiple reaction monitoring m/z 240.0 → 196.3 and m/z 294.0 → 250.2, respectively, with a dwell time of 200 ms for each of the transitions. The standard curve was linear from 20 to 6000 ng/mL. This assay allows quantification of mefenamic acid at a concentration as low as 20 ng/mL in human plasma. The observed mean recovery was 73% for the drug. The applicability of this method for pharmacokinetic studies has been established after successful application during a 12-subject bioavailabity study.

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“…[17][18][19][20] Mefenamic acid [N- (2,3-xylyl)anthranilic acid] (MF) and tolfenamic acid [N-(2-methyl-3-chlorophenyl)anthranilic acid] (TF) are both potent prostaglandin synthetase inhibitors widely used clinically as non-steroidal anti-inflammatory and analgesic-antipyretic drugs. 21,22 For the determination of mefenamic and tolfenamic acids in biological fluids, a number of HPLC methods with spectrophotometric detection have been reported. [21][22][23][24] Although fluorimetry is widely used as a sensitive detection system, there is only one report on the fluorimetric determination of mefenamic acid in pharmaceuticals based on the formation of a fluorescent compound with Al(iii).…”

Section: Introductionmentioning

confidence: 99%

“…21,22 For the determination of mefenamic and tolfenamic acids in biological fluids, a number of HPLC methods with spectrophotometric detection have been reported. [21][22][23][24] Although fluorimetry is widely used as a sensitive detection system, there is only one report on the fluorimetric determination of mefenamic acid in pharmaceuticals based on the formation of a fluorescent compound with Al(iii). 25 In our previous work on terbium sensitized fluorimetry in aqueous solutions, it was mentioned that furosemide interferes with the determination of fluoroquinolone antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric determination of anthranilic acid derivatives based on terbium sensitized fluorescence

Ioannou¹,

Andrikopoulou²,

Glynou³

et al. 1998

Analyst

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Terbium sensitized fluorescence was used to develop a sensitive and simple spectrofluorimetric method for the determination of the anthranilic acid derivatives furosemide and mefenamic and tolfenamic acids. The method makes use of radiative energy transfer from anthranilates to terbium ions in alkaline methanolic solutions. Optimum conditions for the formation of the anthranilate-Tb3+ complexes were investigated. Under optimized conditions, the detection limits are 6 x 10(-9), 1.4 x 10(-8) and 9.0 x 10(-9) mol l-1 for furosemide, mefenamic acids and tolfenamic acid, respectively. The range of application is 2.5 x 10(-8)-5.0 x 10(-5) mol l-1 for all three drugs. The method was successfully applied to the determination of furosemide and mefenamic and tolfenamic acids in serum after extraction of the samples with ethyl acetate, evaporation of the organic layer under a stream of nitrogen at 40 degrees C and reconstitution of the residue with alkaline methanolic terbium solution prior to instrumental measurement. The mean recoveries from serum samples spiked with furosemide (5.0 x 10(-7), 2.0 x 10(-6) and 8.0 x 10(-6) mol l-1), mefenamic acid (3.0 x 10(-6), 9.0 x 10(-6) and 3.0 x 10(-5) mol l-1) and tolfenamic acid (3.1 x 10(-6), 12.5 x 10(-6) and 2.5 x 10(-5) mol l-1) were 96 +/- 8, 101 +/- 5 and 98 +/- 7%, respectively. The within-run precision (RSD) for the method for two serum samples of each drug varied from 2 to 8% and the day-to-day precision for two concentration levels varied from 2 to 13%.

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Simple, rapid and sensitive reversed-phase high-performance liquid chromatographic method for the determination of mefenamic acid in plasma

Cited by 18 publications

References 9 publications

Infrared studies of the polymorphic states of the fenamates

Infrared studies of the polymorphic states of the fenamates

A high performance liquid chromatography–tandem mass spectrometric method for the determination of mefenamic acid in human plasma: application to pharmacokinetic study

Spectrofluorimetric determination of anthranilic acid derivatives based on terbium sensitized fluorescence

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