Simple, rapid, and sensitive quantification of dolichyl phosphates using phosphate methylation and reverse-phase liquid chromatography-high resolution mass spectrometry
Abstract:Dolichyl monophosphates (DolPs) are essential lipids in glycosylation pathways that are highly conserved across almost all domains of life. The availability of DolP is crucial for all glycosylation processes, as these lipids serve as membrane-anchored building blocks that various types of glycosyltransferases use to generate complex post-translational modifications of proteins and lipids. Analysis of DolP species by reverse-phase liquid chromatography-mass spectrometry (RPLC-MS) has remained challenging due to… Show more
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