Simple Radioligand Binding Assay for the Determination of Urinary Scopolamine

Scheurlen, Michael; Bittiger, H.; Ammann, Birgit

doi:10.1002/jps.2600730432

Cited by 14 publications

(2 citation statements)

References 11 publications

(3 reference statements)

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“…18 In addition, several studies have demonstrated constant steadystate urinary excretion of the drug until removal of the patch after 72 hours. 21,22 Thus, once steady state has been reached, the urinary excretion rate will remain constant and cannot therefore provide an explanation for the intersubject variability. 14,23 According to the literature under review and the cumulative data, the variability in plasma scopolamine levels is most probably a combination of differences in dermal and metabolic effects between subjects.…”

Section: Discussionmentioning

confidence: 99%

Safety of Double‐Dose Transdermal Scopolamine

2009

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Section: Discussionmentioning

confidence: 99%

Safety of Double‐Dose Transdermal Scopolamine

2009

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“…The total tropane alkaloid analysis (sum of scopolamine and hyoscyamine) was carried out using tritiated quinuclidinyl benzilate (3H-QNB) radioreceptor assay (14,15). Scopolamine and hyoscyamine are equally potent in this assay at displacing 3H-QNB from its receptor in membranes prepared from rat cortex.…”

Section: Total Tropane Alkaloid Determinationmentioning

confidence: 99%

Variation in the Tropane Alkaloid Content ofHyoscyamus muticusPlants and Cell Culture Clones

Oksman-Caldentey¹,

Vuorela²,

Strauss³

et al. 1987

Planta Med

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Systematic studies were carried out on two different strains (Gatersleben and Cairo) of HYOSCYAMUS MUTICUS L. (Solananaceae) in order to analyse the variation in the contents of the two main tropa-alkaloids in individual plants and protoplast-derived cell culture clones. The hyoscyamine content was determined by radioimmunoassay, the scopolamine content by radio- and enzymeimmunoassay and the total tropane alkaloid content by quinuclidinyl benzilate assay. The development stage of the plant is important for alkaloid production. Clear maximal foliar scopolamine and hyoscyamine contents were reached at the onset of flowering and during the full blooming stage, respectively. In the roots the changes in the production of these alkaloids were not considerable. Hyoscyamine is a major alkaloid in the plants as well as in the cell culture clones. In a few exceptions the scopolamine content was greater than that of hyoscyamine, but this phenomenon was not inherited by the cultured clones derived from these plants. High and low tropane alkaloid production was inherited by the selfed F (1), generation plants. Great variability in these alkaloids was observed among individual plants or clones in the same plant or clone population. There was a significant difference between the Cairo strain and the Gatersleben strain as regards their ability to produce tropane alkaloids. Haploid plants of both strains contained more hyoscyamine and scopolamine than the diploid ones. Hyoscyamine and scopolamine were the main alkaloids in the clones and in the plants. Via QNB-assay an interesting clone was found which contains remarkable amounts of unknown tropane-group alkaloids.

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Liquid chromatographic determination of hyoscine (scopolamine) in urine using solid phase extraction

Whelpton

Hurst

Metcalfe

et al. 1992

Biomedical Chromatography

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A sensitive method for the determination of hyoscine (scopolamine) in urine is described. After concentration and "clean-up" on C18 and CN solid phase extraction columns, hyoscine was quantified by high performance liquid chromatography with coulometric detection (oxidation at +0.9 V). The limit of detection was 5 ng per sample and the precision for 5 mL samples containing 2 ng/mL was 12.3%. The method was applied to urine samples collected from 12 volunteers wearing Scopoderm TTS patches. The mean excretion rate of unmetabolized hyoscine was 0.45 micrograms/h and 87% of the total hyoscine was present as conjugates. Apohyoscine (aposcopolamine) was identified as a urinary metabolite. The significance of this with regard to hyoscine assays is discussed.

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Simple Radioligand Binding Assay for the Determination of Urinary Scopolamine

Cited by 14 publications

References 11 publications

Safety of Double‐Dose Transdermal Scopolamine

Safety of Double‐Dose Transdermal Scopolamine

Variation in the Tropane Alkaloid Content ofHyoscyamus muticusPlants and Cell Culture Clones

Liquid chromatographic determination of hyoscine (scopolamine) in urine using solid phase extraction

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