Brain Edema IX 1994
DOI: 10.1007/978-3-7091-9334-1_31
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Simple Quantitative Evaluation of Blood-Brain Barrier Disruption in Vasogenic Brain Edema

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Cited by 8 publications
(9 citation statements)
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“…[35][36][37] EB is a convenient, simple dye to administer and quantify; however, it binds to serum albumin, making it a 70-kDa protein, which is considered a large molecule, and may ultimately make the use of EB a ''crude'' estimate of vascular permeability. 31,32 Previous studies have used horseradish peroxidase, which is approximately 45 kDa, or a-aminoisobutyric acid, which is only 0.1 kDa. In comparison, EB assays may be less sensitive to detecting subtle disruptions to the BSCB, because it may be too large to leak out.…”
Section: Discussionmentioning
confidence: 99%
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“…[35][36][37] EB is a convenient, simple dye to administer and quantify; however, it binds to serum albumin, making it a 70-kDa protein, which is considered a large molecule, and may ultimately make the use of EB a ''crude'' estimate of vascular permeability. 31,32 Previous studies have used horseradish peroxidase, which is approximately 45 kDa, or a-aminoisobutyric acid, which is only 0.1 kDa. In comparison, EB assays may be less sensitive to detecting subtle disruptions to the BSCB, because it may be too large to leak out.…”
Section: Discussionmentioning
confidence: 99%
“…31,32 EB was allowed to circulate for 20-30 min, and then animals were transcardially perfused with 180 mL of sterile saline. Perfusion of fluid was administered at a rate of 60 mL/min, which is only slightly lower than physiological flow rates, because we did not want to induce vascular injury during this procedure.…”
mentioning
confidence: 99%
“…Preliminary experiments have shown that CHX causes little change in the normal brain because CHX is not expected to penetrate the intact BBB. Therefore, cold‐injured brain was employed as a BBB impaired model in the present study 15 . In brief, male Fischer 344 rats weighing 220–250 g were anesthetized with a mixture of ketamine hydrochloride, xylazine and ethanol.…”
Section: Methodsmentioning
confidence: 99%
“…A cold injury was induced by applying a copper rod (5 mm in diameter), cooled by liquid nitrogen, to the exposed right parietal bone for 60 s. The CHX doses administered in vivo were decided based on the report of Ijiri and Potten 10 . CHX (1 mg/animal) was administered intraperitoneally ( n = 5) at 1 h after cold injury (when BBB breakdown took place) 15 . Rats were killed with ether and their brains were removed 24 h after CHX administration.…”
Section: Methodsmentioning
confidence: 99%
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