Simple procedure for nutrient analysis of coffee plant with energy dispersive X-ray fluorescence spectrometry (EDXRF)

Abstract

Nutrient analysis is used to estimate nutrient content of crop plants to manage fertilizer application for sustained crop production. Direct solid analysis of agricultural and environmental samples by energy dispersive X-ray fluorescence spectrometry (EDXRF) was chosen as alternative technique to evaluate the simultaneous multielemental quantification of the most important essential elements in coffee (Coffea arabica L.) plants. Inductively coupled plasma atomic emission spectrometry and certified reference materials made from leaves were used to calibrate and check the trueness of EDXRF method for the determination of the concentration of several nutrients in coffee leaves and branches. Fluorescence spectrometry proved to be advantageous and presented low cost as loose powder samples could be used. Samples collected from a field experiment where coffee plants were treated with excess of Ni and Zn were used to verify the practical application of the method. Good relationships were achieved between certified values and data obtained by EDXRF, with recoveries ranging from 82 to 117 %.

Antioxidant and antiproliferative activities in different maturation stages of broccoli ( Brassica oleracea Italica) biofortified with selenium

“…The detection was performed using a Si (Li) detector cooled with liquid nitrogen. The intensity of the element Ka counts per second (cps/lA) was determined from the sample X-ray spectrum deconvolution using the EDX Shimadzu software package (Tezotto et al, 2013).…”

Cadmium stress antioxidant responses and root-to-shoot communication in grafted tomato plants

“…Cd content was determined by ICP-OES (Optima 8300, Perkin Elmer). The contents of P, K, Ca, Mg, Cu, Fe, Mn and Zn were determined by X-ray fluorescence method (EDXRF) analysis as described by Tezotto et al (2013), using samples prepared as loose powder. …”

In situ Determination of K, Ca, S and Si in Fresh Sugar Cane Leaves by Handheld Energy Dispersive X-Ray Fluorescence Spectrometry

“…1 The analytical protocol most frequently applied for the determination of macro-and micronutrients in plant materials often requires an a priori test sample decomposition, usually performed by microwave-assisted acid sample dissolution, followed by determination using inductively coupled plasma optical emission spectrometry (ICP OES). 2 Some studies have focused on the use of the direct solid sampling analysis based on X-ray fluorescence spectrometry (XRF), [3][4][5] laser-induced breakdown spectroscopy (LIBS) [6][7][8] or laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) [9][10][11] for assessing the mineral nutrition profile of plants. The most common way of sample presentation by using the aforementioned direct analysis methods involves the interrogation of a previously ground plant material in a pellet form.…”

“…Some studies have focused on the use of the direct solid sampling analysis based on X-ray fluorescence spectrometry (XRF), [3][4][5] laser-induced breakdown spectroscopy (LIBS) [6][7][8] or laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) 9-11 for assessing the mineral nutrition profile of plants. The most common way of sample presentation by using the aforementioned direct analysis methods involves the interrogation of a previously ground plant material in a pellet form.…”