Simple Preparation of Green Fluorescent Protein Conjugated with β-Cyclodextrin in a Site Specific Manner

Suzuki, Miho; Ishimaru, Yoshihiro; Saito, Ayumu; Nishigaki, Koichi

doi:10.2116/analsci.29.811

Cited by 3 publications

(2 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The circular dichroism for the conjugate was carried out with rhodamine B as the guest candidate and indicated possible dye inclusion inside CD to interact with the protein’s chromophore. In summary, there is the possibility to form a conjugate of the 6-iodo-β-CD with the GFP variant; the conjugate can be applied as a sensing device or a drug-delivery system [ 131 ].…”

Section: Cyclodextrin-protein Conjugatesmentioning

confidence: 99%

Cyclodextrins-Peptides/Proteins Conjugates: Synthesis, Properties and Applications

2021

View full text Add to dashboard Cite

Cyclodextrins (CDs) are a family of macrocyclic oligosaccharides mostly composed of six, seven, or eight α-D-glucopyranose units with α-1,4-glycosidic bonds to form toroidal structures. The CDs possess a hydrophilic exterior and hydrophobic interior with the ability to form an inclusion complex, especially with hydrophobic molecules. However, most existing studies are about conjugation CDs with peptide/protein focusing on the formation of new systems. The CD-peptide/protein can possess new abilities; particularly, the cavity can be applied in modulation properties of more complexed proteins. Most studies are focused on drug delivery, such as targeted delivery in cell-penetrating peptides or co-delivery. The co-delivery is based mostly on polylysine systems; on the other hand, the CD-peptide allows us to understand biomolecular mechanisms such as fibryllation or stem cell behaviour. Moreover, the CD-proteins are more complexed systems with a focus on targeted therapy; these conjugates might be controllable with various properties due to changes in their stability. Finally, the studies of CD-peptide/protein are promising in biomedical application and provide new possibilities for the conjugation of simple molecules to biomolecules.

show abstract

Section: Cyclodextrin-protein Conjugatesmentioning

confidence: 99%

Cyclodextrins-Peptides/Proteins Conjugates: Synthesis, Properties and Applications

2021

View full text Add to dashboard Cite

show abstract

“…Consequently, bioprobes for various targets can render normalized FRET signals because of simple measurements of net FRET efficiencies. Preparation processes of bioprobes required no tricky handling even for substitution of imaging target 36 . As introducing obtained bioprobes into cells either with a protein delivery system or direct mixing into culture supernatant was easy and efficient to adapt quantity ratio of each bioprobes introduced considering their sensitivities, this strength, but not weakness, facilitated to repeat the observation of multiple bioprobes simultaneously and acquire corresponding imaging promptly.…”

Section: Introductionmentioning

confidence: 99%

Live imaging of apoptotic signaling flow using tunable combinatorial FRET-based bioprobes for cell population analysis of caspase cascades

Suzuki

Shindo

Yamanaka

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

Understanding cellular signaling flow is required to comprehend living organisms. Various live cell imaging tools have been developed but challenges remain due to complex cross-talk between pathways and response heterogeneities among cells. We have focused on multiplex live cell imaging for statistical analysis to address the difficulties and developed simple multiple fluorescence imaging system to quantify cell signaling at single-cell resolution using Förster Resonance Energy Transfer (FRET)-based chimeric molecular sensors comprised of fluorescent proteins and dyes. The dye-fluorescent protein conjugate is robust for a wide selection of combinations, facilitating rearrangement for coordinating emission profile of molecular sensors to adjust for visualization conditions, target phenomena, and simultaneous use. As the molecular sensor could exhibit highly sensitive in detection for protease activity, we customized molecular sensor of caspase-9 and combine the established sensor for caspase-3 to validate the system by observation of caspase-9 and -3 dynamics simultaneously, key signaling flow of apoptosis. We found cumulative caspase-9 activity rather than reaction rate inversely regulated caspase-3 execution times for apoptotic cell death. Imaging-derived statistics were thus applied to discern the dominating aspects of apoptotic signaling unavailable by common live cell imaging and proteomics protein analysis. Adopted to various visualization targets, the technique can discriminate between rivalling explanations and should help unravel other protease involved signaling pathways.

show abstract

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

Harvey

2018

Mass Spectrometry Reviews

View full text Add to dashboard Cite

This review is the eighth update of the original article published in 1999 on the application of Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2014. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, and arrays. The second part of the review is devoted to applications to various structural types such as oligo- and poly- saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Much of this material is presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. © 2018 Wiley Periodicals, Inc. Mass Spec Rev 37:353-491, 2018.

show abstract

Simple Preparation of Green Fluorescent Protein Conjugated with β-Cyclodextrin in a Site Specific Manner

Cited by 3 publications

References 33 publications

Cyclodextrins-Peptides/Proteins Conjugates: Synthesis, Properties and Applications

Cyclodextrins-Peptides/Proteins Conjugates: Synthesis, Properties and Applications

Live imaging of apoptotic signaling flow using tunable combinatorial FRET-based bioprobes for cell population analysis of caspase cascades

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

Contact Info

Product

Resources

About