Abstract:concentration of FFAs than BTC-6, due to the increased sensitivity of these cells. Mitochondrial function was monitored by measurements of the mitochondrial oxygen consumption (high-resolution respirometry), ATP content (PerkinElmer), mitochondrial membrane potential [flow cytometry (BD)], activity of the caspase-9 (R&D Systems) and ROS generation (DCFH-DA). In preadipocyte cell line: palmitic acid impaired mitochondrial function that was manifested by a decreased intracellular ATP content and an increased ROS… Show more
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