Simple methods to remove microbes from leaf surfaces

Guzmán, J. Paola Saldierna; Nguyen, Kennedy; Hart, Stephen C.

doi:10.1002/jobm.202000035

Cited by 21 publications

(14 citation statements)

References 29 publications

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“…Recently, Saldierna Guzmán et al . (2020) performed a comparison of leaf sterilization protocols for two species, an angiosperm and a gymnosperm. They found that complete removal of the cuticle was required to achieve the sterilization of leaf surface in both species, but that the most effective reagents to remove the cuticle without damaging the integrity of leaf tissues differed between species.…”

Section: Methodological Challenges and Limitations To Study Endophytesmentioning

confidence: 99%

The plant endosphere world – bacterial life within plants

Compant

Cambon

Vacher

et al. 2020

Environmental Microbiology

187

104

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The plant endosphere is colonized by complex microbial communities and microorganisms, which colonize the plant interior at least part of their lifetime and are termed endophytes. Their functions range from mutualism to pathogenicity. All plant organs and tissues are generally colonized by bacterial endophytes and their diversity and composition depend on the plant, the plant organ and its physiological conditions, the plant growth stage as well as on the environment. Plant-associated microorganisms, and in particular endophytes, have lately received high attention, because of the increasing awareness of the importance of host-associated microbiota for the functioning and performance of their host. Some endophyte functions are known from mostly lab assays, genome prediction and few metagenome analyses; however, we have limited understanding on in planta activities, particularly considering the diversity of micro-environments and the dynamics of conditions. In our review, we present recent findings on endosphere environments, their physiological conditions and endophyte colonization. Furthermore, we discuss microbial functions, the interaction between endophytes and plants as well as methodological limitations of endophyte research. We also provide an outlook on needs of future research to improve our understanding on the role of microbiota colonizing the endosphere on plant traits and ecosystem functioning.

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Section: Methodological Challenges and Limitations To Study Endophytesmentioning

confidence: 99%

The plant endosphere world – bacterial life within plants

Compant

Cambon

Vacher

et al. 2020

Environmental Microbiology

187

104

View full text Add to dashboard Cite

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“…All samples were then surface-sterilized by immersion in 1% NaClO solution prepared with DNA-free autoclaved water for 2 min, 70% EtOH for 2 min and rinsed twice in sterile, DNA-free autoclaved water for 5 min. Surface sterilization is a common and effective technique used to prevent the polymerase chain reaction (PCR) amplification of DNA that is found on the surface of tissues [39][40][41][42][43]. It is considered the gold standard [44] and the application of bleach NaClO ensures DNA is denatured [45,46].…”

Section: Methodsmentioning

confidence: 99%

The Microbiome of the Reef Macroalga Sargassum ilicifolium in Singapore

Bollati

Maithani

et al. 2021

Microorganisms

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The large canopy-forming macroalga, Sargassum ilicifolium, provides shelter and food for numerous coral reef species, but it can also be detrimental at high abundances where it outcompetes other benthic organisms for light and space. Here, we investigate the microbial communities associated with S. ilicifolium in Singapore, where it is an abundant and important member of coral reef communities. We collected eight complete S. ilicifolium thalli from eight island locations along an approximate 14 km east-to-west transect. Each thallus was dissected into three separate parts: holdfast, vesicles, and leaves. We then characterized the bacterial communities associated with each part via polymerase chain reaction (PCR) amplification of the 16S rRNA gene V4 region. We then inferred predicted metagenome functions using METAGENassist. Despite the comparatively short distances between sample sites, we show significant differences in microbial community composition, with communities further differentiated by part sampled. Holdfast, vesicles and leaves all harbor distinct microbial communities. Functional predictions reveal some separation between holdfast and leaf communities, with higher representation of sulphur cycling taxa in the holdfast and higher representation of nitrogen cycling taxa in the leaves. This study provides valuable baseline data that can be used to monitor microbial change, and helps lay the foundation upon which we can begin to understand the complexities of reef-associated microbial communities and the roles they play in the functioning and diversity of marine ecosystems.

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“…All samples were rinsed in 0.2 μm filtered water collected from the river and, sediment-free tissues were then immerged in 70% ethanol for 30 s followed by a rinse in sterile Milli-Q water (MoBio Laboratories, Carlsbad, CA, United States). This procedure was repeated three times, with a final rinse in Milli-Q water performed three times to remove any excess of ethanol ( Saldierna Guzmán et al, 2020 ). Fruits were surface sterilised as described above prior to opening with a sterilised razor blade in order to remove the seeds.…”

Section: Methodsmentioning

confidence: 99%

Distinct Endophytic Bacterial Communities Inhabiting Seagrass Seeds

et al. 2021

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Seagrasses are marine angiosperms that can live completely or partially submerged in water and perform a variety of significant ecosystem services. Like terrestrial angiosperms, seagrasses can reproduce sexually and, the pollinated female flower develop into fruits and seeds, which represent a critical stage in the life of plants. Seed microbiomes include endophytic microorganisms that in terrestrial plants can affect seed germination and seedling health through phytohormone production, enhanced nutrient availability and defence against pathogens. However, the characteristics and origins of the seagrass seed microbiomes is unknown. Here, we examined the endophytic bacterial community of six microenvironments (flowers, fruits, and seeds, together with leaves, roots, and rhizospheric sediment) of the seagrass Halophila ovalis collected from the Swan Estuary, in southwestern Australia. An amplicon sequencing approach (16S rRNA) was used to characterize the diversity and composition of H. ovalis bacterial microbiomes and identify core microbiome bacteria that were conserved across microenvironments. Distinct communities of bacteria were observed within specific seagrass microenvironments, including the reproductive tissues (flowers, fruits, and seeds). In particular, bacteria previously associated with plant growth promoting characteristics were mainly found within reproductive tissues. Seagrass seed-borne bacteria that exhibit growth promoting traits, the ability to fix nitrogen and anti-pathogenic potential activity, may play a pivotal role in seed survival, as is common for terrestrial plants. We present the endophytic community of the seagrass seeds as foundation for the identification of potential beneficial bacteria and their selection in order to improve seagrass restoration.

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Simple methods to remove microbes from leaf surfaces

Cited by 21 publications

References 29 publications

The plant endosphere world – bacterial life within plants

The plant endosphere world – bacterial life within plants

The Microbiome of the Reef Macroalga Sargassum ilicifolium in Singapore

Distinct Endophytic Bacterial Communities Inhabiting Seagrass Seeds

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