Simple method for the simultaneous isolation and determination of fumonisin B1 and its metabolite aminopentol-1 in swine liver by liquid chromatography–fluorescence detection

Determination of Fumonisin B1 in animal tissues with immunoaffinity purification

“…The interday variation coefficients obtained for the three tissues were also quite similar (from 2.2 to 12.5%) whatever the FB 1 fortification, confirming the correct reproducibility of the method for FB 1 values comprised between 0.025 and 0.250 g/g. These results are comparable to those obtained in liver by Pagliuca (3.2-15.5% for repeatability and 4-18.4 for the inter-day assay) by using SPE and HPLC combined with fluorescence detection [16].…”

“…Although some peaks were observed in the first part of the chromatogram, they did not interfere with the FB 1 retention time (eluted at around 10 min). These peaks may correspond to substances with a primary amine group that are more polar than FB 1 [16]. The profile of the chromatogram obtained for the kidney and the muscle of duck were quite similar to those obtained for the liver (data not shown).…”

“…The first method uses extraction with strong anion exchange (SAX) cartridges and quantification with HPLC connected to a quadrupole mass spectrometer with electrospray ionisation [15]. The second method is based on solid phase extraction followed by HPLC and fluorescence detection [16].…”

A direct assessment of mycotoxin biomarkers in human urine samples by liquid chromatography tandem mass spectrometry

“…As previously mentioned, DON-3Glu and AFB 1 -N7Gua were synthesized using the protocol described in Wu et al [38] and Egner et al [39] respectively. Hydrolyzed FB 1 (HFB 1 or aminopentol) was synthesized by alkaline hydrolysis as described in Pagliuca et al [40].…”

Fumonisins: oxidative stress-mediated toxicity and metabolism in vivo and in vitro

“…HFB 1 (42-121 µg/ kg) was detected in the liver of weaned piglets fed a diet with FB 1 at 30 mg/kg feed for 6 weeks based on high-performance liquid chromatography (HPLC) combined with fluorescence detection (FL), indicating that the appearance of HFB 1 in the liver clearly originates from the breakdown of FB 1 from dietary origin (Pagliuca et al 2005). Humpf et al (1998) reported that HFB 1 (but not FB 1 , under the conditions tested) can be acylated to N-palmitoyl-HFB 1 (also called N-Pal-HFB 1 or pHFB 1 ) with a V max /km of approximately 1 by ceramide synthase (Humpf et al 1998).…”