Simple method for the quantitative analysis of endogenous folate catabolites p-aminobenzoylglutamate (pABG) and its acetamido (apABG) derivative in human serum and urine by liquid chromatography-tandem mass spectrometry

Sokoro, AbdulRazaq; Etter, Michele L.; Lepage, Joyce; Weist, Benjamin; Eichhorst, Jeff; Lehotay, Denis C.

doi:10.1016/j.jchromb.2005.11.053

Cited by 13 publications

(7 citation statements)

References 17 publications

(15 reference statements)

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“…Our observation of low concentrations of pABG in serum from healthy blood donors (mean 0.07 nmol/L) is in disagreement with a previous report of a mean pABG concentration of 8.9 nmol/L in serum from healthy volunteers (26 ). These results were obtained with serum samples that were acidified without any antioxidants present.…”

Section: Folate Catabolitescontrasting

confidence: 99%

“…The 2 folate catabolites, pABG and apABG, have been detected in urine (36 ) and have also been reported in serum (26 ). Our observation of low concentrations of pABG in serum from healthy blood donors (mean 0.07 nmol/L) is in disagreement with a previous report of a mean pABG concentration of 8.9 nmol/L in serum from healthy volunteers (26 ).…”

Section: Folate Catabolitescontrasting

confidence: 88%

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Liquid Chromatography–Tandem Mass Spectrometry Analysis of Folate and Folate Catabolites in Human Serum

2009

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Section: Folate Catabolitescontrasting

confidence: 99%

Section: Folate Catabolitescontrasting

confidence: 88%

Liquid Chromatography–Tandem Mass Spectrometry Analysis of Folate and Folate Catabolites in Human Serum

2009

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“…Urinary pABG and apABG, which reflect turnover in endogenous folate pools, are frequently measured by chromatography-based methods (HPLC, GC-MS) after preanalytical passage through immobilized FBP affinity columns to remove potential interference from intact folate (258,381). More recent studies reported the development of faster and highly sensitive LC-MS/MS procedures for the quantification of pABG and apABG in urine (382,383) and the use of this procedure for quantifying these catabolites in fasted spot urine as a noninvasive alternative to 24-h urine analysis (384). pABG and apABG in serum can also be measured by LC-MS/MS by using procedures developed for the selective and simultaneous quantification of pABG and apABG and other folate species (5-methyl-THF, 5-formyl-THF, hmTHF, folic acid) (367).…”

Section: Other Biomarker Methodsmentioning

confidence: 99%

Biomarkers of Nutrition for Development—Folate Review

Lynch¹,

Pfeiffer

Georgieff

et al. 2015

The Journal of Nutrition

637

412

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The Biomarkers of Nutrition for Development (BOND) project is designed to provide evidence-based advice to anyone with an interest in the role of nutrition in health. Specifically, the BOND program provides state-of-the-art information and service with regard to selection, use, and interpretation of biomarkers of nutrient exposure, status, function, and effect. To accomplish this objective, expert panels are recruited to evaluate the literature and to draft comprehensive reports on the current state of the art with regard to specific nutrient biology and available biomarkers for assessing nutrients in body tissues at the individual and population level. Phase I of the BOND project includes the evaluation of biomarkers for 6 nutrients: iodine, iron, zinc, folate, vitamin A, and vitamin B-12. This review represents the second in the series of reviews and covers all relevant aspects of folate biology and biomarkers. The article is organized to provide the reader with a full appreciation of folate's history as a public health issue, its biology, and an overview of available biomarkers (serum folate, RBC folate, and plasma homocysteine concentrations) and their interpretation across a range of clinical and population-based uses. The article also includes a list of priority research needs for advancing the area of folate biomarkers related to nutritional health status and development.

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“…Until recently, the analysis procedure has required extensive sample preparation and clean up ( 153 ) , making it unsuitable for clinical or epidemiological use. However, recent innovations using liquid chromatography–MS–MS have simplified this process greatly, such that high-volume analysis of urinary and serum p-amino-benzoylglutamate and acetamido-benzoylglutamate may be both possible and cost effective in the future ( 154 , 155 ) . Analysis of p-amino-benzoylglutamate may also have potential as a biomarker of folate status in degraded blood samples ( 155 ) .…”

Section: Functional Biomarkers Of Folate Statusmentioning

confidence: 99%

Assessment and interpretation of micronutrient status during pregnancy

Wheeler

2008

Proc. Nutr. Soc.

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Accurate assessment of maternal micronutrient status is critical to the prevention of suboptimal micronutrient status and anaemia during pregnancy. Measurement of Fe, folate and vitamin B12status is complicated by adaptive changes to maternal and placental physiology that markedly affect concentrations of circulating micronutrients and their functional biomarkers. Validation of new assessment methods by comparison with gold standards is often prevented by ethical considerations. Antenatal screening in the UK is predominantly concerned with the detection of anaemia, although estimation of maternal Fe stores by serum ferritin at the start of antenatal care may be a more effective preventive strategy. Functional assessment of maternal anaemia is highly problematic, so instead reference data are used for its definition. The effect of mild-to-moderate anaemia on pregnancy outcome is unclear because of the crude nature of its assessment and the influence of confounding factors. Fe-deficient erythropoiesis may be detected by assessment of erythrocyte Zn protoporphyrin and reticulocyte Hb, although such measures may be unavailable in many clinical laboratories. Serum soluble transferrin receptor is highly responsive to tissue Fe deficiency and is less affected by inflammation than most other indicators. Direct inter-assay comparison of serum and erythrocyte folate values is inadvisable since recovery rates differ greatly between methods. Serum total homocysteine is a useful functional biomarker of both folate and vitamin B12status but during pregnancy is influenced by other factors that reduce its sensitivity. Isotope-dilution liquid chromatography–tandem MS and serum holo-transcobalamin provide new opportunities to gain detailed data of folate species and vitamin B12fractions in large samples.

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Simple method for the quantitative analysis of endogenous folate catabolites p-aminobenzoylglutamate (pABG) and its acetamido (apABG) derivative in human serum and urine by liquid chromatography-tandem mass spectrometry

Cited by 13 publications

References 17 publications

Liquid Chromatography–Tandem Mass Spectrometry Analysis of Folate and Folate Catabolites in Human Serum

Liquid Chromatography–Tandem Mass Spectrometry Analysis of Folate and Folate Catabolites in Human Serum

Biomarkers of Nutrition for Development—Folate Review

Assessment and interpretation of micronutrient status during pregnancy

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