Simple method for plant virus detection: effect of antibody immobilization technique

Boltovets, P. N.; Boyko, V. R.; Kostikov, Igor; Dyachenko, N. S.; Snopok, B. A.; Shirshov, Yu.M.

doi:10.1016/s0166-0934(02)00098-8

Cited by 25 publications

(9 citation statements)

References 17 publications

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“…19 SPR is a direct optical-sensing technique that measures the refractive index change due to bio-specific interactions occurring in the vicinity of a thin metal film surface. 20 SPR biosensors for detecting influenza virus 21 and plant virus 22 have been reported. Piezoelectric biosensors consist of mass-sensitive piezoelectric crystals with excitation electrodes.…”

Section: Transducer Elementmentioning

confidence: 99%

Development and Applications of Portable Biosensors

2015

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The significance of microfluidics-based and microelectromechanical systems-based biosensors has been widely acknowledged, and many reviews have explored their potential applications in clinical diagnostics, personalized medicine, global health, drug discovery, food safety, and forensics. Because health care costs are increasing, there is an increasing need to remotely monitor the health condition of patients by point-of-care-testing. The demand for biosensors for detection of biological warfare agents has increased, and research is focused on ways of producing small portable devices that would allow fast, accurate, and on-site detection. In the past decade, the demand for rapid and accurate on-site detection of plant disease diagnosis has increased due to emerging pathogens with resistance to pesticides, increased human mobility, and regulations limiting the application of toxic chemicals to prevent spread of diseases. The portability of biosensors for onsite diagnosis is limited due to various issues, including sample preparation techniques, fluid-handling techniques, the limited lifetime of biological reagents, device packaging, integrating electronics for data collection/analysis, and the requirement of external accessories and power. Many microfluidic, electronic, and biological design strategies, such as handling liquids in biosensors without pumps/valves, the application of droplet-based microfluidics, paper-based microfluidic devices, and wireless networking capabilities for data transmission, are being explored.

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Section: Transducer Elementmentioning

confidence: 99%

Development and Applications of Portable Biosensors

2015

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“…З іншого боку -при подібній обробці на поверхні золота формується заряджений шар з ефективним негативним зарядом, утворений CNугрупованнями, що дозволяє іммобілізувати білкові молекули на поверхні сенсора орієнтовано через позитивно заряд жені амінокислотні угруповання за допомогою іонообміну з відповідними малими протийонами. Для досліджень в області вірусології цей підхід було апробовано в роботі [15], в якій він використовувався для дослідження інфікування вірусом тютюнової мозаїки не характерного для нього господаря -водорості Bracteococcus minor. У роботі [1] даний підхід було застосовано для дослідження вірусної інфекції в динаміці на моделі вірусу тютюнової мозаїки -Nicotiana tabacum.…”

Section: Ncs (тіоціанат)unclassified

Surface plasmon resonance: approaches and perspectives of application for virus-specific interactions investigations

Boltovets¹,

Nesterova²

2016

Mikrobiol. Z.

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“…Usually, the Ag-binding domains of the probe are better accessible to the analyte when the surface-coupling site within the probe is at a distant position from the Ag-capturing site. Within the Ab population, the Ag-binding kinetics upon covalent immobilization remains more uniform and this can affect the biosensor sensitivity positively 67 . This was emphasized by Bonroy et al 68 when they showed for a particular Ab-Ag pair that the optimized fragmentation protocol in combination with an oriented immobilization of Fab fragments on mixed self-assembled monolayers (SAMs) led to a more than two-fold increase of the Ag binding signals compared to randomly covalent immobilized full-length Abs.…”

Section: Immobilization Of Antibody Fragmentsmentioning

confidence: 99%

Antibody Fragments as Probe in Biosensor Development

Saerens

Huang

Bonroy

et al. 2008

Sensors

139

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Today's proteomic analyses are generating increasing numbers of biomarkers, making it essential to possess highly specific probes able to recognize those targets. Antibodies are considered to be the first choice as molecular recognition units due to their target specificity and affinity, which make them excellent probes in biosensor development. However several problems such as difficult directional immobilization, unstable behavior, loss of specificity and steric hindrance, may arise from using these large molecules. Luckily, protein engineering techniques offer designed antibody formats suitable for biomarker analysis. Minimization strategies of antibodies into Fab fragments, scFv or even single-domain antibody fragments like VH, VL or VHHs are reviewed. Not only the size of the probe but also other issues like choice of immobilization tag, type of solid support and probe stability are of critical importance in assay development for biosensing. In this respect, multiple approaches to specifically orient and couple antibody fragments in a generic one-step procedure directly on a biosensor substrate are discussed.

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Simple method for plant virus detection: effect of antibody immobilization technique

Cited by 25 publications

References 17 publications

Development and Applications of Portable Biosensors

Development and Applications of Portable Biosensors

Surface plasmon resonance: approaches and perspectives of application for virus-specific interactions investigations

Antibody Fragments as Probe in Biosensor Development

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