Simple method for O-GlcNAc sensitive detection based on graphene quantum dots

Gao, Li; Wang, Yiwen; Lu, Mengmeng; Fa, Mengmei; Yang, Dong; Yao, Xin

doi:10.1039/c7ra02643a

Cited by 6 publications

(3 citation statements)

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“…Wheat germ agglutinin (WGA) has a long history of being used in O-GlcNAc research. For example, based on the interaction between WGA and O-GlcNAc, an ultrasensitive colorimetric strategy and two biosensor systems , were recently developed for the quantification of protein O-GlcNAcylation levels in cells. However, WGA has a notoriously low affinity for O-GlcNAc (with a dissociation constant ( K d ) of ∼2.5 mM for free GlcNAc) .…”

Section: Characterization Of Individual O-glcnac Proteinsmentioning

confidence: 99%

Analytical and Biochemical Perspectives of Protein O-GlcNAcylation

2021

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Protein O-linked β-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation) is a unique monosaccharide modification discovered in the early 1980s. With the technological advances in the past several decades, great progress has been made to reveal the biochemistry of O-GlcNAcylation, the substrates of O-GlcNAcylation, and the functional importance of protein O-GlcNAcylation. As a nutrient sensor, protein O-GlcNAcylation plays important roles in almost all biochemical processes examined. Although the functional importance of O-GlcNAcylation of proteins has been extensively reviewed previously, the chemical and biochemical aspects have not been fully addressed. In this review, by critically evaluating key publications in the past 35 years, we aim to provide a comprehensive understanding of this important post-translational modification (PTM) from analytical and biochemical perspectives. Specifically, we will cover (1) multiple analytical advances in the characterization of O-GlcNAc cycling components (i.e., the substrate donor UDP-GlcNAc, the two key enzymes O-GlcNAc transferase and O-GlcNAcase, and O-GlcNAc substrate proteins), (2) the biochemical characterization of the enzymes with a variety of chemical tools, and (3) exploration of O-GlcNAc cycling and its modulating chemicals as potential biomarkers and therapeutic drugs for diseases. Last but not least, we will discuss the challenges and possible solutions for basic and translational research of protein O-GlcNAcylation in the future.

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Section: Characterization Of Individual O-glcnac Proteinsmentioning

confidence: 99%

Analytical and Biochemical Perspectives of Protein O-GlcNAcylation

2021

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“…A broadening of O-H stretching at 3292 cm −1 , C = O stretching at 1749 and 1701 cm −1 , and C-OH stretching at 1164 cm −1 was detected in citric acid (precursor). While the synthetic carbon dots exhibit peaks at 1591 and 1398 cm −1 that are thought to be caused by the stretching vibrations of the carboxylate group in symmetric and asymmetric patterns, respectively [ 48 , 49 ]. Furthermore, the broad intense peak at 3429 cm −1 can be ascribed to the O-H stretching vibration of the carbon dots [ 50 , 51 ].…”

Section: Resultsmentioning

confidence: 99%

Polypyrrole/carbon dot nanocomposite as an electrochemical biosensor for liquid biopsy analysis of tryptophan in the human serum of normal and breast cancer women

et al. 2023

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Liquid biopsy analysis represents a suitable alternative analysis procedure in several cases where no tumor tissue is available or in poor patient conditions. Amino acids can play a crucial role in aiding cancer diagnosis. Monitoring of tryptophan (Trp) catabolism can aid in tracking cancer progression. Therefore, a novel nanocomposite was fabricated using overoxidized polypyrrole film doped with nano-carbon dots (nano-CDs) on the pencil graphite electrode (PGE) surface for sensitive evaluation of Trp in human serum. Using square wave voltammetry (SWV), the overoxidized polypyrrole/carbon dots/pencil graphite electrode (Ov-Ox PPy/CDs/PGE) achieved excellent electrochemical catalytic activity for evaluating Trp. The modified electrode, known as Ov-Ox PPy/CDs/PGE, demonstrated superior electrochemical catalytic activity compared to bare PGE, CDs/PGE, PPy/PGE, and PPy/CDs/PGE for evaluation of Trp. The method’s excellent sensitivity was confirmed by the low limits of detection (LOD = 0.003 μmol L−1) and limit of quantitation (LOQ = 0.009 μmol L−1). The biosensor that was developed can measure tryptophan (Trp) levels in the serum of both healthy individuals and female breast cancer patients with high accuracy and sensitivity. The results indicate that there is a significant difference, as shown by the F-test, between healthy individuals and those with breast cancer. This suggests that Trp amino acid could be an essential biomarker for cancer diagnosis. Consequently, liquid biopsy analysis presents a valuable opportunity for early disease detection, particularly for cancer. Graphical abstract

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“…Moreover, the characteristic peak of C=O and C-O of citric acid were found at 1720 and 1207 cm -1 , respectively. Both of the absorption peaks were also significant changed to 1556 and 1380 cm -1 which assigned to be C=O and C-O of GQDs, respectively [62,63]. Interestingly, the typical mode of phenolic of C-OH stretching was attributed at 1078 cm -1 .…”

Section: Preparation Of Lys In Milk Samplementioning

confidence: 90%

Nanomaterial sensors for selective detection of biothiol compounds

Chaicham

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Novel nanomaterial sensors have been designed two different strategies of sensing approach. The first sensing system, the aggregation nanomicelles (TSC and TSD) were synthesized for GSH sensing based on disulfide-cleavaged trigger in the micelle. The result showed a large quenching fluorescent intensity around 587 nm and 528 nm corresponding to TSC and TSD, respectively, upon the addition of GSH in 2.5% DMSO/PBS at pH 6. Additionally, the benefit of TSD is utility for the distance-based fluorescence detection of 3D paper analytical device (3D-?PAD) for the detection of GSH.? Apart from GQDs sensors base, we demonstrated the utility and board applicability of GQDs/AuNPs nanohybrid with measurements of Cysteine (Cys) and Lysine (Lys) using UV-Visible and fluorescent spectroscopy, respectively. For Cys sensing approach, the Cys enable to induce the self-aggregation of GQDs/AuNPs resulting in the color change from red to blue and red shift from 525 nm to 645 nm. On the other hand, Lys exhibited the fluorescence recovery at 450 nm belonging to emission band of GQDs. This is indicative of the displacement of Lys on AuNPs and releasing free GQDs in the solution. This sensor provided a high selectivity toward Cys and Lys with LOD of 5.88 ?M and 16.14 ?M, respectively. Furthermore, GQDs/AuNPs offered an efficient quantitative determination of Cys in urine samples and Lys in milk samples with real time analysis and highly specific detection.

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Simple method for O-GlcNAc sensitive detection based on graphene quantum dots

Abstract: Simple and sensitive method for O-GlcNAc detection in cell lysates based on graphene quantum dots combination; WGA was successfully developed.

Cited by 6 publications

References 50 publications

Analytical and Biochemical Perspectives of Protein O-GlcNAcylation

Analytical and Biochemical Perspectives of Protein O-GlcNAcylation

Polypyrrole/carbon dot nanocomposite as an electrochemical biosensor for liquid biopsy analysis of tryptophan in the human serum of normal and breast cancer women

Nanomaterial sensors for selective detection of biothiol compounds

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