Simple Method for Isolation of Selenomonas ruminantium and Some Nutritional Characteristics of the Species

Tiwari, Abhishek; Bryant, M. P.; Wolfe, R. S.

doi:10.3168/jds.s0022-0302(69)86899-2

Cited by 46 publications

(23 citation statements)

References 8 publications

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“…S. bovis 4/1 was grown aerobically overnight at 37°C in Todd‐Hewitt broth (Imuna, Slovakia) containing 0 and 5 μg Hg 2+ ml −1 in the form of mercuric chloride or anaerobically in the same medium prepared with a gas phase of CO 2 containing 0 and 5 μg Hg 2+ ml −1 . S. ruminantium E32 was also grown anaerobically overnight at 37°C in the selective M medium described by Tiwari et al [13] in the presence of 0, 5 or 50 μg Hg 2+ ml −1 .…”

Section: Methodsmentioning

confidence: 99%

The influence of mercury on the antioxidant enzyme activity of rumen bacteria Streptococcus bovis and Selenomonas ruminantium

Lenártová

Holovská

Javorský

1998

FEMS Microbiology Ecology

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Studies were undertaken to investigate the activity response of the antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GSHPx), glutathione reductase (GR) and mercury reductase (MR) of rumen bacteria Streptococcus bovis and Selenomonas ruminantium following exposure to HgCl2. SOD activity of S. bovis which was considered as Mn‐SOD increased when incubated with 5 μg Hg2+ ml−1 in anaerobic or aerobic conditions. A significant increase in the aerobic activities of GSHPx, GR and MR was observed in the presence of Hg2+. The anaerobic activities of these enzymes were unchanged and increased production of thiobarbituric acid reactive substances was observed. S. ruminantium was tolerant to 10 times higher concentrations of Hg2+ than S. bovis. As a reduction of GSHPx, GR and MR activities after exposure to Hg2+ was observed, we assume that the production of sulfides prevented the toxic effect of mercury on this bacterium.

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Section: Methodsmentioning

confidence: 99%

The influence of mercury on the antioxidant enzyme activity of rumen bacteria Streptococcus bovis and Selenomonas ruminantium

Lenártová

Holovská

Javorský

1998

FEMS Microbiology Ecology

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“…M. elsdenii strains were obtained from rumen contents of lamb (3J, 5J, 7J), calf (2T, 5T) and sheep (4O, 5O), and were previously characterized in our laboratory based on their ability to utilize saccharides, analysis of fermentation end products and Gram staining and cell morphology according to [10]. The strains were grown statically at 37 °C in M10 medium [11] supplemented with fructose (5 g l −1 ) for 16 hours. All cultivations were done anaerobically under a CO 2 atmosphere.…”

Section: Methodsmentioning

confidence: 99%

Different restriction and modification phenotypes in ruminal lactate-utilizing bacteria

Piknová

Filova²,

Javorský

et al. 2004

FEMS Microbiology Letters

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Analysis of restriction and modification activities in lactate-utilizing bacteria belonging to the Megasphaera elsdenii and Mitsuokella multiacida species revealed the presence of GATC-specific, MboI isospecific, restriction-modification (R-M) systems in all strains tested. While restriction endonucleases isolated from M. elsdenii strains were found to be sensitive to Dam methylation, enzymes from M. multiacida cleaved DNA irrespective of Dam methylation. The comparison of type II R-M systems specificities in three closely related lactate-utilizing ruminal bacterial species indicated complete lack of restriction and/or modification enzymes previously characterized from Selenomonas ruminantium in tested M. elsdenii and M. multiacida strains. R-M systems are believed to represent the main defense tool against phage infection. Based on the results of our experiments it could be assumed that M. elsdenii and M. multiacida use the different strategy for bacteriophage protection compared to S. ruminantium.

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“…Selenomonas ruminantium 18D was cultivated anaerobically in selective M medium [5] at 39°C until the late stationary phase of growth was reached (16 h). The cells were harvested by centrifugation (10000 × g, 15 rain, 4°C) and stored at 130 -20°C.…”

Section: Growth Of the Organismmentioning

confidence: 99%

SruI restriction endonuclease from Selenomonas ruminantium

Vanat

1993

FEMS Microbiology Letters

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SruI, specific restriction endonuclease, has been characterized from Selenomonas ruminantium isolated from the rumen of fallow deer. Results from the study demonstrate that S. ruminantium 18D possesses a type II restriction endonuclease, which recognizes the sequence 5'-TTT decreases AAA-3'. The recognition sequence of SruI was identified using digestions on pBR322, pBR328, pUC18, M13mp18RF, pACYC184 and lambda DNA. The cleavage patterns obtained were compared with computer-derived data. SruI recognises the palindromic hexanucleotide sequence and cleaves DNA after the third T in the sequence, producing blunt ends. The purification and characterization of restriction endonuclease SruI presented here is the first described for Selenomonas ruminantium spp. and demonstrates that this microorganism possesses a DNA-cleaving enzyme with the same specificity as DraI or AhaIII.

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Simple Method for Isolation of Selenomonas ruminantium and Some Nutritional Characteristics of the Species

Cited by 46 publications

References 8 publications

The influence of mercury on the antioxidant enzyme activity of rumen bacteria Streptococcus bovis and Selenomonas ruminantium

The influence of mercury on the antioxidant enzyme activity of rumen bacteria Streptococcus bovis and Selenomonas ruminantium

Different restriction and modification phenotypes in ruminal lactate-utilizing bacteria

SruI restriction endonuclease from Selenomonas ruminantium

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