Simple Method for Introducing Elemental Mercury into Biological Growth Systems

Holm, Harvey W.; Cox, Marilyn F.

doi:10.1128/aem.27.3.622-623.1974

Cited by 5 publications

(2 citation statements)

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“…A 500-ml volume of the desired growth medium was placed in each flask and sterilized. After cooling, elemental mercury was added by the procedure of Holm and Cox (7). After a 48-h exposure to elemental mercury, the mercury source was removed, samples were removed for initial chemical analysis, and the flasks were inoculated.…”

Section: Methodsmentioning

confidence: 99%

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Transformation of Elemental Mercury by Bacteria

Holm

Cox

1975

Applied Microbiology

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The fate and impact of elemental mercury in closed bacterial cultures were examined. The quantity of elemental mercury oxidized by bacteria ranged from small amounts for Pseudomonas aeruginosa, P. fluorescens, Escherichia coli, and Citrobacter to essentially all of the added elemental mercury for Bacillus subtilis and B. megaterium. The percentage of the total mercury in the system associated with bacterial cells ranged from 18.6 to 43.2%. Growth of the two Pseudomonas species was inhibited by elemental mercury, whereas growth of the other cultures was not distinguishable from that in mercury-free controls. No methylmercury was formed by the six cultures within 48 h.

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Section: Methodsmentioning

confidence: 99%

“…Jerneliv (8) suggested that the organic carbon in water should increase the rate of oxidation of elemental mercury. We (7) observed that elemental mercury is stable in a sterile, chemically defined growth medium for at least 48 h.…”

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confidence: 90%