Simple method for estimating glycosylated hemoglobins, and its application to evaluation of diabetic patients.

Abstract

Recent reports have suggested that determination of glycosylated hemoglobin may serve as a clinical aid for long-term blood glucose control in diabetes mellitus. We describe a simple procedure for measuring it by ion-exchange chromatography. Hemolysates were subjected to Bio-Rex 70 chromatographic separation on small columns. Percentages in the normal group ranged from 4.7 to 8.8% of total hemoglobin; the mean +/- standard error was 6.61 +/- 0.31%. Values in the diabetic group ranged from 6.9 to 17.4%; the mean was 10.83 +/- 0.34. Plasma glucose concentrations after fasting, plotted vs. the percent of glycosylated hemoglobin, revealed a linear relationship at normal or moderately high glucose concentrations. However, the values for glycosylated hemolgobin approached a plateau with grossly higher plasma glucose concentrations after fasting. Our results support the view that, due to its long half-life, the estimation of glycoylated hemoglobin reflects the integrated glucose concentrations to which the erythrocytes have been previously exposed.

Glycosylated haemoglobin in renal failure

“…This is in agreement with Graf and Porte [12] regarding normal subjects and untreated diabetic patients and the finding of Ditzel and Kjaergaard [13] in newly discovered diabetics. The parabolic relationship between fasting blood-glucose and HbAlc supports the findings of several others [14,15,16] who suggest a saturable system of glycosylation of haemoglobin. It is striking that no correlation was found between HbAI~ and oral glucose tolerance.…”

Models for Plasma Glucose, HbA1c, and Hemoglobin Interrelationships in Patients with Type 2 Diabetes Following Tesaglitazar Treatment

“…15 The likely explanation to the nonlinear relationship is that fasting glucose and time-average glucose are not directly proportional to each other. 15,16 Nonlinear relationship between FPG and HbA1c has also been reported elsewhere, as shown in Figure 7 and described by Chou et al 17 Gender differences in glycemic response, with larger treatment effects for females, have been reported for other PPAR-γ agonists. 18,19 We also found significant gender differences in FPG, where females had a lower EC 50 FPG compared to male patients.…”

Αλληλεπιδραση Μητερας-Παιδιου Στη Διαβητικη Εγκυμοσυνη: Η Επιδραση Των Συστατικων Της Αιμοσφαιρινης, Των Παραγοντων Οξυγονωσεως Και Της Λει- Τουργιας Των Β-Κυτταρων Του Παγκρεατος Στο Βαρος Γεννησεως Των Βρεφων

“…Προσπάθειες νά εξηγήσουν αυτά τά φαινόμενα οδήγησαν μερικούς ερευνητές (Ditzel καί Standi 1975, Kohner 1975, Ditzel 1976) νά διατυπώσουν τήν άποψη δτι ή μικροκυκλοφοριακή διαστολή οφείλεται σέ μία τοπική αύτορυθμιστική αντίδραση στην ύποξία τ(3ν ίστον. (1958), (Schneck καί Schroeder 1961, Trivelli et al 1971, Kynoch καί Lehmann 1977, Chou et al 1978, Abraham et al 1978 Abraham et al (1978) καί Saibene et al (1979).…”