Simple method for determining phthalate diesters and their metabolites in seafood species using QuEChERS extraction and liquid chromatography-high resolution mass spectrometry

Hidalgo-Serrano, Míriam; Borrull, Francesc; Marcé, Rosa María; Pocurull, Eva

doi:10.1016/j.foodchem.2020.127722

Cited by 20 publications

(10 citation statements)

References 35 publications

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“…Satisfactory method performance characteristics in terms of recovery (90%–111%), accuracy (RSD < 13%), LODs (0.3–0.33 μg/L), and LOQs (1.0–100 μg/L) were obtained, which indicate the sensitivity and efficiency of direct UPLC-MS/MS in the PAEs determination. Perhaps MS/MS continues to be the method of choice; liquid chromatography-high resolution mass spectrometry (LC-HRMS) has been recently used for the determination of PAEs and their metabolites in seafood species [ 33 , 111 ] and to dust samples from different indoor environments [ 128 ]. HRMS coupled to LC for PAEs determination allows better selectivity and sensitivity compared to the low resolution of MS due to accurate masses.…”

Section: Analytical Approachesmentioning

confidence: 99%

Comprehensive Insight from Phthalates Occurrence: From Health Outcomes to Emerging Analytical Approaches

Luís

Algarra

Câmara

2021

Toxics

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Phthalates are a group of chemicals used in a multitude of important industrial products (e.g., medical devices, children’s toys, and food packages), mainly as plasticizers to improve mechanical properties such as flexibility, transparency, durability, and longevity of polyvinyl chloride (PVC). The wide occurrence of phthalates in many consumer products, including foods (e.g., bottled water, soft drinks, wine, milk, and meat) brings that most people are exposed to phthalates every day, which raises some concerns. Adverse health outcomes from phthalates exposure have been associated with endocrine disruption, deformities in the human reproductive system, increased risk of preterm birth, carcinogen exposure, among others. Apprehension related to the health risks and ubiquitous incidence of phthalates in foods inspires the development of reliable analytical approaches that allow their detection and quantification at trace levels. The purpose of the current review is to provide information related to the presence of phthalates in the food chain, highlighting the health risks associated with their exposure. Moreover, an overview of emerging extraction procedures and high-resolution analytical approaches for a comprehensive quantification of phthalates is presented.

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Section: Analytical Approachesmentioning

confidence: 99%

Comprehensive Insight from Phthalates Occurrence: From Health Outcomes to Emerging Analytical Approaches

Luís

Algarra

Câmara

2021

Toxics

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“…During the optimisation process, to avoid high ion suppression in the final extracts due to the lack of a clean-up step, and to prevent the loss of the compounds by evaporation, 1 mL of the ACN phase was transferred to a 10 mL volumetric flask and taken up to the final volume with ultrapure water, instead of preconcentrating the extracts before the injection. The solvent volume and vortex time conditions for the extraction were set based on our previous experience [ 32 ]. It should be noted that, since the lipidic content of the samples can affect the efficiency of the extraction, two seafood species with different lipid percentages were chosen for the optimisation: hake (low lipid content) and mackerel (high lipid content).…”

Section: Resultsmentioning

confidence: 99%

“…For a quick and simple clean-up, we tested Lipifiltr ® push-thru purification cartridges, which are specifically designed to remove lipids from fatty QuEChERS extracts [ 32 , 33 ]. However, all the target compounds were completely retained in the cartridges, so this strategy was promptly dismissed.…”

Section: Resultsmentioning

confidence: 99%

Determination of Organophosphate Ester Metabolites in Seafood Species by QuEChERS-SPE Followed by LC-HRMS

Hidalgo-Serrano¹,

Borrull²,

Pocurull³

et al. 2022

Molecules

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Organophosphate triesters are compounds widely used in industries and are ubiquitous in the environment, where they can be transformed into organophosphate diesters. Some organophosphate diesters are also used by industry. Several studies suggest organophosphate diesters can have toxic effects for reproduction, and hazardous and mutagenic properties. Due to the impact these compounds can have on marine biota and human beings through the consumption of fish and shellfish, it is necessary to study their presence in widely consumed seafood species. We therefore developed an analytical method for determining six of the most common organophosphate diesters in seafood. The procedure is based on the Quick, Easy, Cheap, Effective, Rugged and Safe extraction method and a solid phase extraction clean-up, followed by liquid chromatography coupled to high-resolution mass spectrometry. The method was optimised and validated for seafood with different lipid content, providing satisfactory relative recoveries (from 89 to 138%) and limits of detection (1.0–50 ng g−1 dry weight), as well as repeatability values (RSD% (n = 5, 100 ng g−1 (dry weight)) lower than 15%. Eight seafood species were analysed using this method and two organophosphate diesters were detected and quantified in all the samples, demonstrating the suitability of the method.

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“…19,20 In recent years, several QuEChERS-based methods have been optimized for extracting different analytes, such as antibiotics, mycotoxins, PAHs, and sulphonamides [20][21][22][23] in food and environmental matrices; these methods also included phthalates in samples of marine biota. 12,24,25 Since the QuEChERS technique has been the method used to analyse food and environmental samples, it has been modied and improved over the years. One of these modications is the miniaturization of the technique, micro-QuEChERS, which has several advantages in relation to conventional extraction techniques, it is less time-consuming, cheaper, and uses fewer chemicals and solvents so it is considered a green method.…”

Section: Introductionmentioning

confidence: 99%

Optimization of micro-QuEChERS extraction coupled with gas chromatography-mass spectrometry for the fast determination of phthalic acid esters in mussel samples

et al. 2023

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Simple method for determining phthalate diesters and their metabolites in seafood species using QuEChERS extraction and liquid chromatography-high resolution mass spectrometry

Cited by 20 publications

References 35 publications

Comprehensive Insight from Phthalates Occurrence: From Health Outcomes to Emerging Analytical Approaches

Comprehensive Insight from Phthalates Occurrence: From Health Outcomes to Emerging Analytical Approaches

Determination of Organophosphate Ester Metabolites in Seafood Species by QuEChERS-SPE Followed by LC-HRMS

Optimization of micro-QuEChERS extraction coupled with gas chromatography-mass spectrometry for the fast determination of phthalic acid esters in mussel samples

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