Simple Mass-screening Methods for Resistance of Tomato to Fusarium oxysporum f. sp. lycopersici

Abstract

Abstract. This study was carried out to establish the simple mass-screening methods for resistant tomato to Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (FOL). Root dip inoculation method has been used in many studies on the resistance of tomato to disease. On the other hand, in mass-screening for resistant tomato to Fusarium wilt, the inoculation method is time-consuming and laborious procedure. Disease development of two FOL isolates on two cultivars of tomato according to inoculation method including root dip, tip and scalpel methods were investigated. In compatible interaction, tomato seedlings of each cultivar inoculated by tip method showed the lower and more variable disease severities than by root dip method. Whereas the seedlings by scalpel method represented clear resistant and susceptible responses to Fusarium wilt as root dip method. The resistance degree of each cultivar inoculated with FOL isolates by scalpel method was hardly affected by the tested incubation temperature and inoculum concentration. On the basis of the results, we suggest scalpel inoculation method as an efficient mass-screening method for resistant of tomato cultivars to Fusarium wilt. Roots of tomato seedlings at two-leaf stage grown in plastic cell tray were injured with scalpel and then spore suspension (more than 1 × 10 7 conidia ․ mL Jones and Overman, 1971;Jones and Woltz, 1972;Weststeijin, 1973;Woltz and Jones, 1973)

Identification of a Potential ISR Determinant from Pseudomonas aeruginosa PM12 against Fusarium Wilt in Tomato

“…W.C. Snyder et H.N. Hansen (FOL) is an alarming disease, causing yield losses up to 25% ( Fravel et al, 2005 ; Park et al, 2013 ). Disease control strategies include use of resistant tomato varieties with cultural, chemical and biological control ( Agrios, 2005 ; Pottorf, 2006 ).…”