Simple LC-MS/MS method using core-shell ODS microparticles for the simultaneous quantitation of edoxaban and its major metabolites in human plasma

Ariizumi, Saki; Naito, Takafumi; Hoshikawa, Kohei; Akutsu, Shunta; Saotome, Masao; Maekawa, Yuichiro; Kawakami, Junichi

doi:10.1016/j.jchromb.2020.122121

Cited by 6 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the ‘gold standard’ for determining the plasma levels of DOAC is liquid chromatography-tandem mass spectrometry (LC-MS/MS). LC-MS/MS is an advanced analytical method used to measure plasma concentrations of all DOACs [ 22 , 23 , 24 , 25 ]. This technique involves detecting the drug molecules in a pretreated plasma sample.…”

Section: Introductionmentioning

confidence: 99%

Stability of Direct Oral Anticoagulants Concentrations in Blood Samples for Accessibility Expansion of Chromogenic Assays

et al. 2023

View full text Add to dashboard Cite

Background and Objectives: Direct oral anticoagulants (DOACs) are used for minimising the risk of thromboembolic events. In clinical practice, there is no need to measure DOAC concentration in the routine. Nevertheless, there are cases where such measurements are necessary, as the European Society of Cardiology’s guideline recommends. However, determining DOAC levels is not available for everyone due to chromogenic assay availability limitations from sample storage problems, as tests are performed only in a few healthcare settings. This study aimed to assess whether more applicable storage conditions could be used for transportation to provide chromogenic assays for outpatient healthcare and other hospitals’ practices. Materials and Methods: Chromogenic assays measuring anti-FXa (for rivaroxaban and edoxaban) and anti-FIIa (for dabigatran) were used. Concentrations were determined immediately after blood collection as baseline value: (1) after the storage of citrated whole blood in refrigerator (+2–8 °C); (2) of citrated plasma in refrigerator (+2–8 °C); and (3) of citrated frozen plasma (−20 °C) on the third and seventh days of storage. Acceptable change limits were considered stable if the deviation did not exceed ±20% of the baseline value. Results: The median (Cl 95%) baseline value of rivaroxaban was 168 (147–236) ng/mL; of dabigatran 139 (99–178) ng/mL; and of edoxaban—174 (135–259) ng/mL. The median deviation from a baseline value stored as citrate whole blood samples (+2–8 °C) was 5.4% and 3.4%; as citrated plasma (+2–8 °C) was 0.4% and −0.6%; and as citrated frozen plasma (−20 °C) was −0.2% and 0.2% on the third and seventh days of storage, respectively. Conclusions: Our data suggest that whole blood samples stored in a refrigerator, as well as citrated plasma samples stored in both the refrigerator and freezer, preserve DOAC concentration stable at +2–8 °C or −20 °C for up to 7 days, and are suitable for transportation, except for low-concentration samples.

show abstract

Section: Introductionmentioning

confidence: 99%

Stability of Direct Oral Anticoagulants Concentrations in Blood Samples for Accessibility Expansion of Chromogenic Assays

et al. 2023

View full text Add to dashboard Cite

show abstract

“…It is indicated mainly for the treatment of pulmonary embolism, deep vein thrombosis, and for prevention of systemic embolism and stroke in patient with nonvalvular atrial fibrillation [4,7]. Searching literature, several methods were reported for the determination of EDX in pharmaceutical formulation and biological fluids including: HPLC methods [8][9][10][11], spectrophotometric methods [12,13], LC-MS/MS [14,15] and UPLC-MS/ MS methods [16][17][18][19][20]. However, no electrochemical method has been described for the selective determination of EDX.…”

Section: Introductionmentioning

confidence: 99%

A Novel Nanoparticles‐based Electrochemical Sensing Platform for Sensitive Detection of Oral Anticoagulant; Edoxaban in Human Plasma

et al. 2022

View full text Add to dashboard Cite

During COVID-19 pandemic, coagulopathy have been reported as a potential threat to most infected patients. Edoxaban (EDX) is a direct oral anticoagulant that is recently added to most COVID-19 treatment protocols either as therapy or prophylaxis. Herein, a novel nanoparticles modified glassy carbon potentiometric sensor was developed for the selective quantitation of EDX in human plasma. The electrochemical performance of the proposed sensor was assessed and compared to nanoparticle free sensor. An enhancement in electrode performance including: detection limit (3.39 × 10 À 6 mol L À 1 ), response time (8.0 � 2.0 s) and improved selectivity. The proposed sensor was able to determine EDX in pure form, pharmaceutical formulation and human plasma.

show abstract

Investigation of the electrochemical properties of edoxaban using glassy carbon and boron-doped diamond electrodes and development of an eco-friendly and cost effective voltammetric method for its determination

Kiliç,

Aslan,

Levent

2024

Analytical Biochemistry

View full text Add to dashboard Cite

Simple LC-MS/MS method using core-shell ODS microparticles for the simultaneous quantitation of edoxaban and its major metabolites in human plasma

Cited by 6 publications

References 19 publications

Stability of Direct Oral Anticoagulants Concentrations in Blood Samples for Accessibility Expansion of Chromogenic Assays

Stability of Direct Oral Anticoagulants Concentrations in Blood Samples for Accessibility Expansion of Chromogenic Assays

A Novel Nanoparticles‐based Electrochemical Sensing Platform for Sensitive Detection of Oral Anticoagulant; Edoxaban in Human Plasma

Investigation of the electrochemical properties of edoxaban using glassy carbon and boron-doped diamond electrodes and development of an eco-friendly and cost effective voltammetric method for its determination

Contact Info

Product

Resources

About