Simple hormonal regulation of somatic embryogenesis and/or shoot organogenesis in caryopsis cultures of Pogonatherum paniceum (Poaceae)

Wang, Wenguo; Zhao, Xin; Zhuang, Guoqing; Wang, Shenghua; Chen, Fang

doi:10.1007/s11240-008-9414-9

Cited by 32 publications

(19 citation statements)

References 44 publications

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“…As in other monocot species (Zhang et al 2007;Wang et al 2008), different types of calluses were produced in U. brizantha cv. Marandu, here named primary calluses, embryogenic calluses, and opaque white compact structures.…”

Section: Discussionmentioning

confidence: 69%

“…High auxin concentrations rapidly change the metabolism of cells resulting in the development of callus (Dudits et al 1995). The auxin 2,4-D alone or in combination with other plant growth regulators has been widely used for the induction of somatic embryogenesis in many grasses such as Cenchrus ciliares (Colomba et al 2006), Chloris gayana (Gondo et al 2007), Cynodon dactylon (Zhang et al 2007), Zoysia matrella (Dhandapani et al 2008), Eremochloa ophiuroids (Barampuram et al 2009), Pogonatherum paniceum (Wang et al 2008), and U. brizantha (Cabral et al 2011). However, other synthetic auxins such as picloram have not been tested for regeneration of tropical grasses from the Urochloa genus.…”

Section: Discussionmentioning

confidence: 99%

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Optimization of somatic embryogenesis and in vitro plant regeneration of Urochloa species using picloram

Takamori

Neto

Vieira

et al. 2015

In Vitro Cell.Dev.Biol.-Plant

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The establishment of an efficient methodology for regeneration in Urochloa spp. via somatic embryogenesis is an essential component of genetic engineering technology. The aim of this study was to evaluate different concentrations of growth regulators on callus induction, somatic embryogenesis, and plant regeneration of Urochloa spp. using mature seeds as the initial explant. Firstly, different concentrations (1, 2, 4, and 8 mg/L) of 2,4-D or picloram were tested using U. brizantha cv. Marandu. In a second step, the above concentrations of picloram were also used to evaluate its effect on the morphogenetic potential in different genotypes: U. brizantha cvs. Xaraés and Piatã, U. decumbens cv. Basilisk, U. humidicola cv. Llanero, and U. ruziziensis cv. Ruziziensis. There was no significant difference between concentrations of 2,4-D or picloram for the production of primary calluses in U. brizantha cv. Marandu, ranging from 58.5 to 69.2%. However, embryogenic calluses induced under picloram showed higher percentage of shoot formation after transferring to MS medium with 2 mg/L BA. A higher number of shoots were produced in mature seed cultures of U. decumbens cv. Basilisk and U. brizantha cvs. Marandu and Xaraés supplemented with 1 mg/L picloram. No albino plants were regenerated using picloram. The successful regeneration of green and morphologically normal plants opens the possibility of using this protocol to obtain transgenic plants in Urochloa spp.

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Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Optimization of somatic embryogenesis and in vitro plant regeneration of Urochloa species using picloram

Takamori

Neto

Vieira

et al. 2015

In Vitro Cell.Dev.Biol.-Plant

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“…Generally, auxins are used for callus induction and proliferation, and both cytokinins and auxins are required for redifferentiation of callus into organized cells (Wang et al 2008). In this present study, callus was successfully induced in the presence of auxin (IAA) and cytokinin (Zn, Kin).…”

Section: Resultsmentioning

confidence: 80%

Somatic embryogenesis and shoot organogenesis in the medicinal plant Pulsatilla koreana Nakai

Lin

Zhao

Hong

et al. 2010

Plant Cell Tiss Organ Cult

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We have developed a system for the in vitro regeneration of pasqueflowers (Pulsatilla koreana Nakai). The system was based on somatic embryogenesis and shoot organogenesis. Over a growth period of 6 weeks, multiple shoots were initiated from leaf, petiole, and pedicel explants on Murashige and Skoog (MS) medium containing 0.5 mg l -1 indole-3-acetic acid (IAA) and zeatin (Zn), kinetin (Kin), or 6-benzyladenine (BA). We achieved 100% of adventitious shoot induced when petiole and pedicel explants were cultured on MS, 0.5-2.0 mg l -1 Zn, and 0.5 mg l -1 IAA. Somatic embryos developed from the explants and generated shoots on MS medium containing 0.25 mg l -1 Zn and 0.5 mg l -1 IAA. Globular and heartshaped stages of somatic embryos were observed. Histological studies have revealed the stages of development of somatic embryos. For propagation and growth, the regenerated shoots from organogenic or embryogenic calluses were transferred to MS medium containing either (1) 1.5 mg l -1 Zn and 0.05 mg l -1 IAA or (2) 1.0 mg l -1 BA and 0.05 mg l -1 IAA. After the length of the shoots reached 3 cm, the shoots initiated by organogenesis as well as those initiated by somatic embryogenesis were transferred to the root induction medium. After 2 months of culture in halfstrength MS with 1.5 mg l -1 a-naphthalene acetic acid (NAA), the rooting ratio was 93%. Finally, the rooted plantlets were acclimatized in a mixture of mountain soil and perlite.

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“…Generally, auxins are used for callus induction and proliferation, and both cytokinins and auxins are required for redifferentiation of callus into organized cell (Wang et al 2008). In this study, successful induction of callus was achieved in the presence of NAA, and callus was then subsequently induced to undergo somatic embryogenesis in the presence of BA and NAA.…”

Section: Callus Inductionmentioning

confidence: 89%

Induction of somatic embryogenesis and organogenesis in Oldenlandia umbellata L., a dye-yielding medicinal plant

Siva

Rajasekaran

Mudgal

2009

Plant Cell Tiss Organ Cult

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Oldenlandia umbellata L., commonly known as ''Indian madder'', is an ancient Indian herb valued as a source of red color dye and various medicinal products. In this study, successful protocols have been developed for induction of somatic embryogenesis and organogenesis in O. umbellata. Emerging young leaves, shoot apices, and stems were used as explants, grown on Murashige and Skoog (MS) media supplemented with various auxins, including indole acetic acid, indole butyric acid, napthaleneacetic acid (NAA), and 2,4-Dichlorophenoxyacetic acid, each at levels ranging between 0.1 and 0.5 mg/l, cytokinins, including benzyladenine (BA) and kinetin, each at concentration ranging between 0.5 and 5 mg/l, with and without coconut milk (CM) at levels of 0.5-5%. For callus induction, NAA at 2.5 mg/l was optimal; while, for rapid embryogenic callus induction, 0.2 mg/l NAA, 0.5 mg/l BA, and 0.1% CM induced the highest frequency (95.86%). Shoots developed upon transfer of embryogenic calli to MS medium containing 1.5 mg/l BA, 0.3 mg/l NAA and 1% CM. For root induction, 0.3 mg/l NAA and 1.0% CM promoted highest and earliest rooting.

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Simple hormonal regulation of somatic embryogenesis and/or shoot organogenesis in caryopsis cultures of Pogonatherum paniceum (Poaceae)

Cited by 32 publications

References 44 publications

Optimization of somatic embryogenesis and in vitro plant regeneration of Urochloa species using picloram

Optimization of somatic embryogenesis and in vitro plant regeneration of Urochloa species using picloram

Somatic embryogenesis and shoot organogenesis in the medicinal plant Pulsatilla koreana Nakai

Induction of somatic embryogenesis and organogenesis in Oldenlandia umbellata L., a dye-yielding medicinal plant

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