Simple green production of silver nanoparticles facilitated by bacterial genomic DNA and their antibacterial activity

Abstract

Green synthesis of AgNPs has gained many research interests as a low cost and eco-friendly approach. This work reported on the use of bacterial genomic DNA as the alternative biopolymer for a green production of AgNPs under a neutral pH. Although both ssDNA and dsDNA could function as stabilizing agents during the synthesis process, the ssDNA, generated by pre-heating the dsDNA at 100 °C, was more efficient to produce AgNPs with higher yield as determined by the intensity of the surface plasmon resonance (SPR) peak of silver at 475 nm. The obtained AgNPs were spherical with the average diameter of 15.0 ± 7.6 nm, which their identity was confirmed by X-ray diffraction, high-resolution transmission electron microscopy, and selected area electron diffraction analyses. The synthesized AgNPs exhibited the potent antibacterial activity against both Escherichia coli and Staphylococcus aureus with the minimum bactericidal concentrations at 250 and 500 μg/ml, respectively, suggesting their potential antibacterial activity against both Gram-negative and Gram-positive bacteria.

Antimicrobial Activity and Sorption Behavior of Al2O3/Ag Nanocomposites Produced with the Water Oxidation of Bimetallic Al/Ag Nanoparticles

“…The MIC of the silver nanoparticles has been reported to range from 0.25 µg × mL −1 [ 54 ] to 550 µg × mL −1 [ 55 ], and depends on the method of nanoparticle production, their size and accompanying impurities. The wet, chemically synthesized silver nanoparticles [ 56 ] showed antimicrobial activity at a concentration of 250 µg × mL −1 . Taking into account this value, isobolograms were plotted for the synthesized nanostructures ( Figure 17 ) to evaluate the nature of the mutual influence of the components on the antibacterial activity of the samples.…”

In vivo formation of spherical and rod lead nanoparticles in root cells of water velvet (Azolla pinnata)

“…7C also indicated the dominant mass composition of Pb in the Pb‐treated roots in addition to C and O components. The presence of Cu element was mainly from the carbon‐coated copper grid used in this analysis 45.…”

Smartphone-Based Paper Microfluidic Detection of Paraquat Mediated by Citrate-Capped Silver Nanoparticles

“…3b). The spectrum of the dispersed AgNPs showed the characteristic SPR peak of silver at 418 nm [30]. With the presence of paraquat, the SPR peak at 418 nm was decreased in a dose-dependent response and the induction of absorbance intensity in the wavelength region of 550-800 nm.…”