Simple fluorescent enzyme immunoassay for detection and quantification of hepatitis C viremia

Tanaka, Takeshi; Lau, Johnson Y.N.; Mizokami, Masashi; Orito, Etsuro; Tanaka, Eiji; Kiyosawa, Kendo; K, Yasui; Ohta, Yoshikazu; Hasegawa, Akira; Tanaka, Satoshi; Kohara, Michinori

doi:10.1016/0168-8278(95)80043-3

Cited by 115 publications

(110 citation statements)

References 11 publications

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“…During the past decade, several HCV Ag tests have been developed as potential alternatives to the HCV RNA assay (3). The first was developed by Tanaka et al (28) in 1995, and then Aoyagi et al (2) developed a new and 100-fold more sensitive test in 1999. In previously reported studies, HCV Ag was detected 1 day later than HCV RNA in patients undergoing seroconversion (5,6,20).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, nucleic acid amplifications are labor-intensive and time-consuming methods and have the risk of laboratory contamination; for these reasons, amplification methods are not suitable for widespread use in most laboratories, especially in developing countries (1,4,11,23,27). Therefore, the HCV Ag assay is needed as a supplemental or preconfirmatory test to preconfirm anti-HCV results and distinguish false-positive results from the accurate ones because it is easy to perform and reliable, has high specificity and sensitivity rates, is cost-effective, is able to shorten the duration of the time to diagnosis of infection in patients during the window period, and has a lower risk of laboratory contamination than assays based on nucleic acid amplification technology (31,28). During the past decade, several HCV Ag tests have been developed as potential alternatives to the HCV RNA assay (3).…”

Section: Discussionmentioning

confidence: 99%

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Comparison of a Newly Developed Automated and Quantitative Hepatitis C Virus (HCV) Core Antigen Test with the HCV RNA Assay for Clinical Usefulness in Confirming Anti-HCV Results

et al. 2011

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Hepatitis C virus (HCV) is a global health care problem. Diagnosis of HCV infection is mainly based on the detection of anti-HCV antibodies as a screening test with serum samples. Recombinant immunoblot assays are used as supplemental tests and for the final detection and quantification of HCV RNA in confirmatory tests. In this study, we aimed to compare the HCV core antigen test with the HCV RNA assay for confirming anti-HCV results to determine whether the HCV core antigen test may be used as an alternative confirmatory test to the HCV RNA test and to assess the diagnostic values of the total HCV core antigen test by determining the diagnostic specificity and sensitivity rates compared with the HCV RNA test. Sera from a total of 212 treatment-naive patients were analyzed for anti-HCV and HCV core antigen both with the Abbott Architect test and with the molecular HCV RNA assay consisting of a reverse transcription-PCR method as a confirmatory test. The diagnostic sensitivity, specificity, and positive and negative predictive values of the HCV core antigen assay compared to the HCV RNA test were 96.3%, 100%, 100%, and 89.7%, respectively. The levels of HCV core antigen showed a good correlation with those from the HCV RNA quantification (r ‫؍‬ 0.907). In conclusion, the Architect HCV antigen assay is highly specific, sensitive, reliable, easy to perform, reproducible, cost-effective, and applicable as a screening, supplemental, and preconfirmatory test for anti-HCV assays used in laboratory procedures for the diagnosis of hepatitis C virus infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparison of a Newly Developed Automated and Quantitative Hepatitis C Virus (HCV) Core Antigen Test with the HCV RNA Assay for Clinical Usefulness in Confirming Anti-HCV Results

et al. 2011

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“…Culture media and intracellular core concentrations were quantified using ELISA as previously described. 30,49,50 Extracellular core protein was concentrated by polyethylene glycol precipitation as follows: 300 µL of 48% polyethylene glycol 6,000 were added to 1,500 µL culture medium and incubated under rocking overnight at 4°C. The precipitates were pelleted by centrifugation (14,000g for 15 minutes), dissolved in 50 µL of citrated sodium chloride (NaCl 0.5%, sodium citrate 20 mmol/L), and then mixed with 50 µL of 10 mol/L urea and 0.5 mol/L sodium hydroxide.…”

Section: Methodsmentioning

confidence: 99%

Hepatitis C virus core protein binds to apolipoprotein AII and its secretion is modulated by fibrates

et al. 1999

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Hepatitis C virus (HCV) is the major etiologic agent of post-transfusion and sporadic non-A, non-B hepatitis. 1 Persistent HCV infection, which develops in at least 70% to 80% of infected patients, often progresses to chronic hepatitis and is highly correlated with the development of cirrhosis and hepatocellular carcinoma. 2-4 Neither a vaccine, nor effective therapy are currently available for HCV, other than interferon alfa combined with ribavirin. 5,6

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“…This is because it is easy to perform, reliable, has a high specificity and sensitivity rate, is cost effective, is able to shorten the duration of the diagnosis of patients during the window period and has a lower risk of laboratory contamination than assays based on nucleic acid amplification technology. 45,50 During the past decade, several HCV Ag tests have been developed as potential alternatives to HCV RNA assay. 5 The first was developed by Tanaka et al 45 1995, and then Aeyogi et al 1 developed a new and 100-fold more sensitive test.…”

Section: S T C O M P a R E D W I T H H C V R N A P R O V E D T H A mentioning

confidence: 99%

“…45,50 During the past decade, several HCV Ag tests have been developed as potential alternatives to HCV RNA assay. 5 The first was developed by Tanaka et al 45 1995, and then Aeyogi et al 1 developed a new and 100-fold more sensitive test. In previous studies, the HCV Ag was detected one day after the HCV RNA in patients undergoing seroconversion.…”

Section: S T C O M P a R E D W I T H H C V R N A P R O V E D T H A mentioning

confidence: 99%

An Overview of the Laboratory Assay Systems and Reactives Used in the Diagnosis of Hepatitis C Virus (HCV) Infections

Keşli¹

2012

Trends in Immunolabelled and Related Techniques

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Simple fluorescent enzyme immunoassay for detection and quantification of hepatitis C viremia

Cited by 115 publications

References 11 publications

Comparison of a Newly Developed Automated and Quantitative Hepatitis C Virus (HCV) Core Antigen Test with the HCV RNA Assay for Clinical Usefulness in Confirming Anti-HCV Results

Comparison of a Newly Developed Automated and Quantitative Hepatitis C Virus (HCV) Core Antigen Test with the HCV RNA Assay for Clinical Usefulness in Confirming Anti-HCV Results

Hepatitis C virus core protein binds to apolipoprotein AII and its secretion is modulated by fibrates

An Overview of the Laboratory Assay Systems and Reactives Used in the Diagnosis of Hepatitis C Virus (HCV) Infections

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