Simple Enzymatic Determination of Polysaccharide (Glycogen) Content of Animal Tissues

Murat, J.C.; Serfaty, A

doi:10.1093/clinchem/20.12.1576

Cited by 201 publications

(56 citation statements)

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“…The protein content of all samples was determined by Kjeldahl analysis (protein= 6.25 N) using dried tissue samples. The glycogen content of liver and white muscle tissue was determined using the method described by Murat & Serfaty (1974), with liberated glucose being measured by the glucose-oxidase method (Peridochrom Glucose GOD-PAP, Boehringer-Mannheim).…”

Section: Carcass and Tissue Analysissupporting

confidence: 67%

The regulation of endogeneous energy stores during starvation and refeeding in the somatic tissues of the golden perch

Collins¹,

Anderson²

1995

Journal of Fish Biology

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Adult golden perch Macquaria ambigua were fed to satiety, starved for up to 210 days, or starved for 150 days then fed to satiety for 60 days to investigate the utilization of energy stores in response to food deprivation and re-feeding. Golden perch sequentially mobilize energy from hepatic tissue, extra-hepatic lipid, and finally muscle components in response to food deprivation. The relative size of the liver was significantly reduced by 30 days after the onset of food deprivation due to the simultaneous mobilization of lipid, protein and glycogen reserves. These stores were renewed rapidly within 30 days by satiety feeding. Mobilization of lipid stores in perivisceral fat bodies occurred between 30 and 60 days of food deprivation. These deposits were also renewed upon re-feeding, although not as rapidly as liver reserves. The glycogen content of the epaxial muscle was reduced by the 60th day of food deprivation but subsequently increased indicating the mobilization of other energy reserves. The concentration of muscle lipid decreased after 90 days of food deprivation. The only significant response in body composition observed in the fish fed to satiety throughout the study was an increase in the relative size of the perivisceral fat bodies. The results of this study suggest that golden perch are well adapted to cope with extended periods of food deprivation, storing energy as perivisceral fat when food is readily available and having a clearly sequential process for mobilizing energy when food is scarce which largely protects the integrity of the musculature. C) 1995 The Fisheries Society of the British Islo

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Section: Carcass and Tissue Analysissupporting

confidence: 67%

The regulation of endogeneous energy stores during starvation and refeeding in the somatic tissues of the golden perch

Collins¹,

Anderson²

1995

Journal of Fish Biology

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“…Oligosaccharides were also measured spectrophotometrically with Megazyme Raffinose-series oligosaccharides/D-Glucose assay kit (Megazyme International, Co.). The method followed for liver glycogen was according to Murat & Serfaty (1974) and liver fat according to Folch et al (1957). Trypsin inhibitor activity was determined with the method described by Smith et al (1980).…”

Section: ó 2010 Blackwell Publishing Ltdmentioning

confidence: 99%

Effects of dietary inclusion of peas, chickpeas and faba beans on growth, feed utilization and health of gilthead seabream (Sparus aurata)

Adamidou

Nengas

Henry

et al. 2011

Aquaculture Nutrition

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Three legumes [field peas (P), chickpeas (CP) and faba beans (B)] at two inclusion levels [170 g kg)1 (L) and 350 g kg)1 (H)] were evaluated in a 13-week experiment with triplicate groups of 92.6 +/- 5.0 g gilthead seabream (Sparus aurata L.). A control diet included wheat meal, fishmeal (FM) and a mixture of plant ingredients as protein sources. Diets were formulated to be isonitrogenous and isoenergetic and processed in a twin-screw extruder. Restricted feeding was applied (15 g kg)1 of body weight) and growth, haematology and histology parameters were evaluated. Decreased, but not significant, growth values were observed for all diets including legumes compared to the control. Poorer feed conversion ratio values were observed for both P diets and for high level B diet. Liver glycogen increased with increasing starch level, but hepatosomatic index did not differ significantly for any of the diet treatments. Histological examination of internal organs showed no pathological abnormalities that could be related to nutritional treatment. The study indicated that the tested legumes are ingredients that could be used in farmed seabream diets up to 350 g kg)1 without negative effects replacing other carbohydrate sources and part of FM

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“…Casein (Sigma, C-8654) was used as a reference material. Digestible carbohydrate in feed and faeces and glycogen in sampled tissues was determined using an enzymatic method after Hemre et al (1989) modified from Murat & Serfaty (1974) and Holm et al (1986). Glucose was subsequently measured spectrophotometrically as NADPH at 340 nm after a hexokinase/glucose-6-phosphate dehydrogenase reaction by use of an automated analyser (Technicon, RA-1000, Tarrytown, NY, USA).…”

Section: Composition Analysismentioning

confidence: 99%

Maximum limits of organic and inorganic mercury in fish feed

Berntssen¹,

Hylland

Julshamn³

et al. 2004

Aquac Nutrition

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The relatively high levels of mercury found in fish feeds might form a fish health and food safety risk. The present study aims to establish sublethal toxic threshold levels in fish and assess feed‐fillet transfer of dietary mercury. Atlantic salmon (Salmo salar L.) parr were fed for 4 months on fish meal‐based diets supplemented with mercuric chloride (0, 0.1, 1, 10 or 100 mg Hg kg−1 dry weight (DW)) or methylmercuric chloride (0, 0.1, 0.5, 5 or 10 mg MeHg kg−1 DW). At the end of the experiment, dietary inorganic mercury mainly accumulated in intestine (80% of body burden) and assimilation was low (6%). In contrast, methylmercury readily accumulated in internal organs and muscle (80% of body burden) and had a relatively high assimilation (23%). Highest accumulation of dietary inorganic mercury was observed in the gut and kidney. Fish fed 10 mg Hg kg−1 had an early (after 2 months) significant increase in renal metallothionein (MT) level and intestinal cell proliferation, followed by intestinal pathological conditions after 4 months of exposure. At 100 mg Hg kg−1, intestinal and renal function were reduced as seen from the significantly reduced protein and glycogen digestibility and increased plasma creatinine levels. For dietary methylmercury (MeHg), highest accumulation was found in blood and muscle. Intestinal cell proliferation and liver MT significantly increased at 5 mg MeHg kg−1 after 2 months of exposure. At the end of the experiment, blood haematology was significantly affected in fish fed 5 mg MeHg kg−1 and these fish exceeded the current food safety limit for mercury. Tissue MT induction and intestinal cell proliferation appeared to be useful and quantifiable early indicators of toxic mercury exposures. Based on the absence of induction of these early biological markers such as MT and cell proliferation, nonobserved effect levels (NOELs) could be set to 0.5 mg Hg kg−1 for dietary methylmercury and 1 mg Hg kg−1 for inorganic mercury. Lowest observed effect levels (LOELs) levels could be set to 5 mg kg−1 for methylmercury and 10 mg Hg kg−1 for inorganic mercury.

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Simple Enzymatic Determination of Polysaccharide (Glycogen) Content of Animal Tissues

Cited by 201 publications

References 0 publications

The regulation of endogeneous energy stores during starvation and refeeding in the somatic tissues of the golden perch

The regulation of endogeneous energy stores during starvation and refeeding in the somatic tissues of the golden perch

Effects of dietary inclusion of peas, chickpeas and faba beans on growth, feed utilization and health of gilthead seabream (Sparus aurata)

Maximum limits of organic and inorganic mercury in fish feed

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