Simple Engineering of Polymer–Nanoparticle Hybrid Nanocapsules

Sciortino, Flavien; Casterou, Gérald; Éliat, Pierre-Antoine; Troadec, Marie‐Bérengère; Gaillard, Cédric; Chevance, Soizic; Kahn, Myrtil L.; Gauffre, Fabienne

doi:10.1002/cnma.201600155

Cited by 15 publications

(24 citation statements)

References 31 publications

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“…We found that at 100 μg/ml of PLL–Au–Fe 3 O 4 and 10 min laser exposure, a ΔT of 15 ± 7.6 °C was achieved. The current study reveals the effectiveness of using PLL–Au–Fe 3 O 4 as a potent MRI-visible photosensitizer during the laser irradiation of cancer cells [ 45 , 61 , 62 ]. The noticeable combination of properties of PLL–Au–Fe 3 O 4 with the local application of NIR laser irradiation offers a promising approach for the photothermal ablation therapy of breast cancer cells following internalization inside cells.…”

Section: Discussionmentioning

confidence: 99%

Polymer coated gold-ferric oxide superparamagnetic nanoparticles for theranostic applications

et al. 2018

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BackgroundEngineered inorganic nanoparticles (NPs) are essential components in the development of nanotechnologies. For applications in nanomedicine, particles need to be functionalized to ensure a good dispersibility in biological fluids. In many cases however, functionalization is not sufficient: the particles become either coated by a corona of serum proteins or precipitate out of the solvent. We show that by changing the coating of magnetic iron oxide NPs using poly-l-lysine (PLL) polymer the colloidal stability of the dispersion is improved in aqueous solutions including water, phosphate buffered saline (PBS), PBS with 10% fetal bovine serum (FBS) and cell culture medium, and the internalization of the NPs toward living mammalian cells is profoundly affected.MethodsA multifunctional magnetic NP is designed to perform a near-infrared (NIR)-responsive remote control photothermal ablation for the treatment of breast cancer. In contrast to the previously reported studies of gold (Au) magnetic (Fe3O4) core–shell NPs, a Janus-like nanostructure is synthesized with Fe3O4 NPs decorated with Au resulting in an approximate size of 60 nm mean diameter. The surface of trisoctahedral Au–Fe3O4 NPs was coated with a positively charged polymer, PLL to deliver the NPs inside cells. The PLL–Au–Fe3O4 NPs were characterized by transmission electron microscopy (TEM), XRD, FT-IR and dynamic light scattering (DLS). The unique properties of both Au surface plasmon resonance and superparamagnetic moment result in a multimodal platform for use as a nanothermal ablator and also as a magnetic resonance imaging (MRI) contrast agent, respectively. Taking advantage of the photothermal therapy, PLL–Au–Fe3O4 NPs were incubated with BT-474 and MDA-MB-231 breast cancer cells, investigated for the cytotoxicity and intracellular uptake, and remotely triggered by a NIR laser of ~ 808 nm (1 W/cm2 for 10 min).ResultsThe PLL coating increased the colloidal stability and robustness of Au–Fe3O4 NPs (PLL–Au–Fe3O4) in biological media including cell culture medium, PBS and PBS with 10% fetal bovine serum. It is revealed that no significant (< 10%) cytotoxicity was induced by PLL–Au–Fe3O4 NPs itself in BT-474 and MDA-MB-231 cells at concentrations up to 100 μg/ml. Brightfield microscopy, fluorescence microscopy and TEM showed significant uptake of PLL–Au–Fe3O4 NPs by BT-474 and MDA-MB-231 cells. The cells exhibited 40 and 60% inhibition in BT-474 and MDA-MB-231 cell growth, respectively following the internalized NPs were triggered by a photothermal laser using 100 μg/ml PLL–Au–Fe3O4 NPs. The control cells treated with NPs but without laser showed < 10% cell death compared to no laser treatment controlConclusionCombined together, the results demonstrate a new polymer gold superparamagnetic nanostructure that integrates both diagnostics function and photothermal ablation of tumors into a single multimodal nanoplatform exhibiting a significant cancer cell death.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0405-7)...

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Section: Discussionmentioning

confidence: 99%

Polymer coated gold-ferric oxide superparamagnetic nanoparticles for theranostic applications

et al. 2018

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“…Both Hybridosome® nanocapsules and electro-click chemistry are emergent concepts useful for providing functional yet versatile materials. 21,26 Hybridosomes are a new class of hybrid nanocapsules that combine the features of both the inorganic nanoparticles and the polymer chains contained in their shells. Here Hybridosomes based on maghemite IONPs and PAA-C≡CH have been used as clickable building blocks.…”

Section: Resultsmentioning

confidence: 99%

“…[18][19][20] Here we report on the one-pot construction of hybrid hollow nanocapsule films, that are able to release their encapsulated cargo in response to either a pH or an electrochemical stimulus. The recently reported 100 nm diameter hybrid nanocapsules, named Hybridosomes®, 21,22 were used as hollow building blocks for assembling the film by (Figure 1a, S-1 and Video S-1), Hybridosomes represent a new class of hollow nanocarriers with several levels of versatility arising from the individual characteristics of their organic and inorganic components and from their hollow structure. For instance, these versatile nanocapsules can be prepared from iron oxide nanoparticles (IONPs), gold nanoparticles, Quantum Dots (QDs) and their mixtures.…”

Section: Introductionmentioning

confidence: 99%

Electro-click construction of hybrid nanocapsule films with triggered delivery properties

Sciortino

Rydzek

Grasset

et al. 2018

Phys. Chem. Chem. Phys.

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Hollow nanocapsules (named Hybridosomes®) possessing a polymer/nanoparticle shell were used to covalently construct hybrid films in a one-pot fashion. The alkyne bearing organic/inorganic Hybridosomes® were reticulated with azide bearing homobifunctional polyethyleneglycol (PEG) linkers, by using an electro-click reaction on F-SnO (FTO) electrodes. The coatings were obtained by promoting the Cu(i)-catalyzed click reaction between alkyne and azide moieties in the vicinity of the electrode by the electrochemical generation of Cu(i) ions. The physicochemical properties of the covalently reticulated hybrid films obtained were studied by SEM, AFM, UV-vis and fluorescence spectroscopy. The one-pot covalent click reaction between the nanocapsules and the PEG linkers in the film did not affect the desirable features of the Hybridosomes® i.e. their hollow nanostructure their chemical versatility and their pH-sensitivity. Consequently, both the composition and the cargo-loading of the Hybridosomes® films could be tuned, demonstrating the versatility of these hybrid coatings. For example, the Hybridosome® films were used to encapsulate and release a bodipy fluorescent probe in response to either a pH drop or the application of an oxidative +1 V potential (vs. Ag/AgCl) at the substrate. By advancing the field of electro-synthesized films a step further toward the design of complex physicochemical interfaces, these results open perspectives for multifunctional coatings where chemical versatility, controllable stability and a hollow nanostructure are required.

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“…All details about the synthesis of the hybridosomes® were described previously. 17 Briefly, the process consists in three steps (Figure 1). First, liquid droplets are coated with the hydrophobic nanoparticles in a water/THF (80:20) mixture forming the primary nanoparticle shell (Step 1).…”

Section: Resultsmentioning

confidence: 99%

“…Recently, our group introduced a simple and robust procedure to elaborate hybrid polymer/nanoparticles capsules (diameter ~ 100 nm), that were coined hybridosomes®. 17 The shell of the capsules is made from inorganic nanoparticles crosslinked by polymers. Interestingly, hybridosomes made from superparamagnetic iron oxide nanoparticles (IONPs) (maghemite) and crosslinked with poly(acrylic acid) (PAA) showed excellent properties as MRI contrast agent and can easily encapsulate hydrophobic compounds.…”

Section: A Introductionmentioning

confidence: 99%

Structure and elasticity of composite nanoparticle/polymer nanoshells (hybridosomes®)

et al. 2017

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Our group recently introduced a new process to synthesize nanoparticle shells of about 100 nm, named "hybridosomes®". Here, the structure and mechanical properties of hybridosomes® made from iron oxide nanoparticles and poly(acrylic acid) are characterized using TEM, AFM and an osmotic compression technique. For the latter, the size distribution of the hybridosomes is monitored by nanoparticle tracking analysis (NTA) in the presence of poly(ethylene glycol)s of different molecular weights. It is found that the size of the hybridosomes® can be tuned from ca. 80 nm to over 110 nm by adjusting the amount of nanoparticles and that their shell consists of a single layer of nanoparticles, with a porous structure. The size of the pores is estimated from osmotic compression experiments at ca. 4000 g mol. The mechanical properties are measured both at the ensemble level using size measurements under osmotic pressure and at the single nanoparticle level by atomic force microscopy nanoindentation. Both osmotic and AFM experiments are analyzed in the framework of the continuum elastic theory of thin shells and yield a value of Young's modulus of the order of MPa.

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Simple Engineering of Polymer–Nanoparticle Hybrid Nanocapsules

Cited by 15 publications

References 31 publications

Polymer coated gold-ferric oxide superparamagnetic nanoparticles for theranostic applications

Polymer coated gold-ferric oxide superparamagnetic nanoparticles for theranostic applications

Electro-click construction of hybrid nanocapsule films with triggered delivery properties

Structure and elasticity of composite nanoparticle/polymer nanoshells (hybridosomes®)

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