Simple device for continuous diffusion destaining of polyacrylamide gels

Gathercole, L.J.; Klein, L.

doi:10.1016/0003-2697(71)90364-2

Cited by 11 publications

(2 citation statements)

References 9 publications

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“…The gels were then destained by continuous diffusion in methanol/acetic acid/water (2:3:35, by vol.) (Gathercole & Klein, 1971). (b) As described by Weber & Osborn (1969).…”

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confidence: 97%

Molecular weight and subunit size of rabbit mammary-gland fatty acid synthetase

Grunnet¹,

Knudsen²

1978

Biochemical Journal

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1. The molecular weights of fatty acid synthetases isolated from lactating rabbit, rat, cow and goat mammary glands were estimated by sucrose gradient centrifugation and compared by chromatography on Sepharose 6B. 2. The values obtained for all four enzymes were in the same range (0.40 X 10(6)-0.55 X 10(6)) as that found for other mammalian and avian fatty acid synthetases. The molecular weight found for the rabbit mammary enzyme therefore differs from published values of approx. 0.9 X 10(6). 3. The molecular weights of the subunits of these four synthetases were 225000-242000. Again, the value for the rabbit mammary enzyme differs from published values.

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“…The gels were then destained by continuous diffusion in methanol/acetic acid/water (2:3:35, by vol.) (Gathercole & Klein, 1971). (b) As described by Weber & Osborn (1969).…”

mentioning

confidence: 97%

Molecular weight and subunit size of rabbit mammary-gland fatty acid synthetase

Grunnet¹,

Knudsen²

1978

Biochemical Journal

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“…The gels were stained with Coomassie blue at a concentra tion of 0.025%(w/v) and were destained by diffusion over a period of 2-3 days, using a simple circulating diffusion destainer [5].…”

Section: Experimental Methodsmentioning

confidence: 99%

Surface Proteins of the Erythrocyte Membrane Effect of Aging

Conrad¹,

Penniston²

1974

Vox Sanguinis

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The protein composition of human erythrocyte membranes was analyzed by discontinuous gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). A protein with a monomer mass of 95,000 daltons and the glycoprotein of highest monomer mass were both altered by extensive pronase digestion of whole erythrocytes. This confirmed that these two proteins were exposed to the external surface of the erythrocyte. Membranes prepared from fresh blood were compared with membranes prepared from blood stored for 3 weeks under blood bank conditions. In membranes from stored blood, a single major protein band, the surface protein of 95,000 daltons, was so extensively denatured that it was not monomerized by 0.1% SDS, and migrated much more slowly through the gel. When the membrane was dissolved in 1.0% SDS, this band was completely monomerized, and the electrophoresis pattern was indistinguishable from that produced by membranes prepared from fresh blood. Treatment with dithiothreitol or mercaptoethanol did not solubilize this band. Electrophoresis under the same conditions, followed by staining by the periodic acid-Schiff base technique showed that the denaturable protein was not a glycoprotein, and that the surface glycoprotein was not affected by aging. This glycoprotein migrated in the same position when membranes from fresh and stored blood were compared.

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An improved method for the analysis of the RNA-directed DNA polymerase reaction by polyacrylamide gels

McCormick

Calhoun

1973

Analytical Biochemistry

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Simple device for continuous diffusion destaining of polyacrylamide gels

Cited by 11 publications

References 9 publications

Molecular weight and subunit size of rabbit mammary-gland fatty acid synthetase

Molecular weight and subunit size of rabbit mammary-gland fatty acid synthetase

Surface Proteins of the Erythrocyte Membrane Effect of Aging

An improved method for the analysis of the RNA-directed DNA polymerase reaction by polyacrylamide gels

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