Simple Determination of Silver Nanoparticles Concentration as Ag⁺ by Using ISE as Potential Alternative to ICP Optical Emission Spectrometry

Abstract

The inductively coupled plasma optical emission spectrometry (ICP‐OES) is a high resolution spectroscopic technique based on the emission intensity at specific wavelengths of a given element within the sample to obtain both qualitative and quantitative information about ions concentration in aqueous solution. The concentration of Ag+ in aqueous solution from negatively charged silver nanoparticles (AgNPs) has been determined by ion selective electrode (ISE) analysis at room temperature. The tested AgNPs solutions have been electrochemically prepared without using any coating agent. The obtained data have been compared to those obtained from the same samples by using ICP‐OES. The good correlation between the two techniques highlights that ISE could be successfully applied as valid alternative to ICP‐OES to determine the silver ion concentration in the investigated conditions. High level of reproducibility, accurate measurements and affordability represent the main advantages of the method described herein.

Structure and Properties of Electrochemically Synthesized Silver Nanoparticles in Aqueous Solution by High-Resolution Techniques

“…Moreover, ORP analysis revealed a value of +400 mV associated with silver nanoclusters in comparison to −90 mV obtained from the DTT standard solution, suggesting oxidative rather than reductive properties of the investigated AgNPs. Furthermore, the absence of free silver ions was demonstrated by ISE analysis [ 43 ].…”

The membrane depolarization and increase intracellular calcium level produced by silver nanoclusters are responsible for bacterial death

“…No change in absorbance and λ max value was observed after 1 h of incubation of SUNCs at pH 3.5 in 0.01 M acetate buffer 22 . A drastic decrease of the plasmonic spectrum absorbance, without change of the λ max , was observed after nitric acid treatment (0.1 M, pH < 1) for 10 min at 25 °C 22 .The complete disappearance of the ARGIRIUM-SUNCs UV–Vis spectrum is obtained only after 60 min of nitric acid (0.1 M final concentration) reaction at 60 °C 23 . After each synthesis, larger nano clusters were removed by flushing the solution through 0.1 μm syringe filter devices (Whatman CYCLPR) and subsequent centrifugation at 13.023× g for 15 min.…”

Silver nanoclusters with Ag2+/3+ oxidative states are a new highly effective tool against phytopathogenic bacteria

“…The synthesis method is protected by the European patent: EP-18181873.3. The physicochemical properties of ARGIRIUM-SUNCs, as determined by transmission electron microscope (TEM), X-ray electron scanning microscopy (XR-SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), matrix-assisted laser desorption/ionization time-of-flight (MALDI TOF), dynamic light scattering (DLS), ultraviolet–visible spectrophotometry (UV–Vis), ion selective electrode (ISE), are: a very small size, 1.79 nm ± 1.004 (ultra nano cluster), (Z-potential), plasmon resonance spectrum (λ max at 410 nm) and nonspherical shape, stability at acidic pH (2–12) (Gasbarri et al 2019 ).…”