Simple determination of peroxyl radical‐trapping capacity

Bartosz, Grzegorz; Janaszewska, Anna; Ertel, Danuta; Bartosz, Małgorzata

doi:10.1080/15216549800204042

Cited by 43 publications

(42 citation statements)

References 3 publications

(4 reference statements)

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“…The data indicate that the ability of raw and processed lentil seeds antioxidants to scavenge peroxyl radicals is smaller than the ability to scavenge ABTS9 + radicals. This may originate from the fact that a relative potential of antioxidants to scavenge ABAP derived peroxyl radicals is different [17] than their TAEC [24,29]. TEAC reflects concentration of Trolox solution with an equivalent antioxidant potential to a standard concentration of the compound under investigation.…”

Section: Prtc Of Phosphate-buffered and Methanolic Extractsmentioning

confidence: 99%

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Contribution of low‐molecular‐weight antioxidants to the antioxidant capacity of raw and processed lentil seeds

Fernández-Orozco

Zieliński²,

Piskuła

2003

Nahrung

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In this study four cultivars of lentil originating from Spain were examined: cv Paula, cv Agueda, cv Almar and cv Alcor. Since consumption of these seeds after heat treatment and as sprouts has been popularised, the impact of cooking (up to 30 min) and germination process (in dark, at 25 degrees C, for up to 4 days) on peroxyl radical-trapping capacity (PRTC) and Trolox-equivalent antioxidant capacity (TEAC) of the processed seeds was addressed. Also, changes in the content of low-molecular-weight antioxidants (LMWA) and soluble proteins in the course of cooking and germination were studied. The analyzed LMWAwere: total phenolics, tocopherols (alpha-T, beta-T, gamma-T, delta-T), reduced glutathione, and L-ascorbic acid. On the basis of the results obtained, the contribution of LMWA and soluble proteins to the PRTC and TEAC of raw, cooked, and germinated lentil seeds was calculated by multiple mean values for the content of investigated compounds and their relative potential with respect to Trolox. The results showed avery high molar percentage contribution of phenolic compounds and low contribution of tocopherols, glutathione, soluble proteins, and ascorbate (only in germinated seeds) to the total TEAC and total PRTC calculated as a sum of data provided for phosphate-buffered and 80% methanolic extracts of raw and processed lentil seeds.

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Section: Prtc Of Phosphate-buffered and Methanolic Extractsmentioning

confidence: 99%

“…The measurements were carried out according to Bartosz et al [17]. The assay was performed as follows: 0.1 M sodium phosphate buffer, pH 7.0, was warmed to 37 8C and added to a cuvette to obtain 3 mL of the final reaction volume.…”

Section: Measurement Of Prtcmentioning

confidence: 99%

Contribution of low‐molecular‐weight antioxidants to the antioxidant capacity of raw and processed lentil seeds

Fernández-Orozco

Zieliński²,

Piskuła

2003

Nahrung

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“…1 (Altai bouquet balm). The composition was as follows [16,17,18]: plant extracts of St. John's wort herb, licorice root, root Hedysarum tea, rose hips, grass meadow geranium, chaga mushroom, sugar syrup, citric acid (acidifier), potassium sorbate (preservative), and sodium benzoate (preservative) [19,20,21]. Under the influence of unfavorable factors, such as foreign chemicals, the concentration of oxyradicals increases sharply and under certain conditions may exceed the ability of the protective antioxidant system to withstand the ravages of these reactive molecules [22][23][24].…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Effect of Nonalcoholic Syrups Based on Wild-Growing Herbal Raw Material

Aleks¹,

Tekuteva²,

Son³

et al. 2016

Biol Med (Aligarh)

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“…Erythrocyte catalase (EC 1.1.11.6) activity was measured according to Beers et al [8]. The antioxidative activity of the blood plasma was determined via the method of Bartosz et al [9], based on the reduction of the preformed cation radical of 2,2,azinobis (3-ethylbenzothiazoline-6-sulphonic acid) by the blood plasma.…”

Section: Antioxidative Enzymesmentioning

confidence: 99%

Carvedilol modifies antioxidant status of patients with stable angina

Kowalski

Banach

Barylski

et al. 2008

Cellular and Molecular Biology Letters

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Abstract:The aim of this study was to evaluate the effect of carvedilol on the enzymatic antioxidative defence and plasma antioxidative activity in patients with stable angina. The study comprised 30 patients, aged 37-49 years with stable angina. Patients received carvedilol in escalating doses of 12.5 mg/24 h, 25 mg/24 h, and 50 mg/24 h for 4 weeks each. The control group was matched for age and gender, and consisted of 12 healthy volunteers, aged 39-49 years. Blood samples were collected from the cubital vein before and 4, 8 and 12 weeks after the therapy from the patients and once from the control group. For all the subjects, the superoxide dismutase (SOD-1), glutathione peroxidase (GSH-Px), catalase (CAT) activities in the erythrocytes and the antioxidant activity of the blood plasma were determined. The enzymatic antioxidative defence was significantly decreased in patients with stable angina in comparison to the healthy subjects. During the carvedilol therapy, an increase in the SOD-1, GSH-Px and CAT activities was observed. Moreover, 8 and 12 weeks after carvedilol therapy, the GSH-Px activity did not differ significantly from that observed in the group of healthy subjects. Carvedilol also increased the plasma antioxidative activity in patients with stable angina, but its level remained significantly lower than in the control group. In conclusion, carvedilol enhances antioxidant defense mechanisms in patients with chronic stable angina pectoris.

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Simple determination of peroxyl radical‐trapping capacity

Cited by 43 publications

References 3 publications

Contribution of low‐molecular‐weight antioxidants to the antioxidant capacity of raw and processed lentil seeds

Contribution of low‐molecular‐weight antioxidants to the antioxidant capacity of raw and processed lentil seeds

Antioxidant Effect of Nonalcoholic Syrups Based on Wild-Growing Herbal Raw Material

Carvedilol modifies antioxidant status of patients with stable angina

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