Simple detection of bacterioplankton using a loop-mediated isothermal amplification (LAMP) assay: First practical approach to 72 cases of suspected drowning

Simple Visualized Detection Method of Virulence-Associated Genes of Vibrio cholerae by Loop-Mediated Isothermal Amplification

“…In this study, our results indicated that positive amplicons were initially detected at a reaction time of 30 min with the loop primers, which was 20-min faster than those without the loop primers. The significance of optimal concentrations of each pair of primers in the LAMP system has been pointed out in previous studies (Okada et al, 2010;Srisuk et al, 2010;Lau et al, 2011;Kakizaki et al, 2018), in which a 1.6 µM of the inner primers, 0.2 µM of the outer primers, and 0.8 µM of the loop primers was often used with an optimal ratio 8:1:4 (µM/µM/µM) of the three primers. In this study, our data showed that 1.60 µM of inner primers, 0.05 µM of the outer primers, and 0.2 µM of the loop primers were optimum for the sssvLAMP method with a final ratio of 32:1:4 (µM/µM/µM), showing fourfold less amount of the outer and loop primers.…”

Determination of a criminal suspect using environmental plant DNA metabarcoding technology

“…Plant DNA has a high potential providing de nitive evidence during criminal investigations. With the advent of DNA metabarcoding, it has recently been used to nd out body dumping site (Yang et al, 2015), residence of unknown human body (Liu et al, 2019), drowning site (Fang et al, 2019), and con rmation of suspected drowning (Kakizaki et al, 2018). Unfortunately, such applications are still very rare due to three main challenges.…”

Application of a Statistical Model to Forecast Drowning Deaths in Iran

“…Drowning occurs during daily or recreational activities, such as swimming, boating, fishing, etc. People living in different areas may also be exposed to various causes of drowning, such as sea, lake, river, canal, pond, pool, bathtub, and so on [8]. Predisposing factors, like high-risk behaviors (alcohol consumption and no use of lifesaving equipment), are also different in various parts of the world, possibly influenced by cultural issues [9].…”