Simple Colorimetric Method for Cholinesterase-inhibitor Screening in Gastric Content by Using Phytoesterase Enzyme from Kidney Bean

Kaewmaroeng, Thanawat; Wunnapuk, Klintean; Chockjamsai, Manoch; Sringarm, Korawan; Hongsibsong, Surat

doi:10.3923/pjbs.2020.904.910

Cited by 2 publications

(1 citation statement)

References 21 publications

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“…The majority of them used animals to produce the antibodies [6,7]. Several enzymeinhibition-based techniques and devices have been developed, including optical colorimetric assays, uorometric assays, electrochemical biosensors, rapid test cards, and micro uidic devices [8][9][10][11][12]. Most of them were developed using the cholinesterase enzyme.…”

Section: Introductionmentioning

confidence: 99%

Developing an In-House Colorimetric Method for Detecting Organophosphate and Carbamate Residue Using Cricket Cholinesterase

Wongta,

Hongsibsong,

Anand

et al. 2023

Preprint

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Background Pesticides such as organophosphates and carbamates are used to control pests, leaving residues on fruits and vegetables. The assessment of residues in food materials is required for consumer and agricultural worker health protection. The purpose of this study was to develop an in-house colorimetric method for detecting organophosphate and carbamate residues using cricket cholinesterase that can be used as a low-cost, simple-to-use, and widely used test. Result The method's parameters, including substrate specificity, kinetics, and incubation time, were optimized. The specificity and purification factor of the produced ChE were 0.77 M/min/mg and 7.16-fold, respectively. The LOD for detecting organophosphates and carbamates was in the range of 0.002–0.877 ppm. The validity of the method yielded the following results: relative sensitivity of 95%, relative specificity of 90%, relative accuracy of 93%, positive predictive value of 95%, and negative predictive value of 90%. Conclusion The developed method is simple and inexpensive, and it can analyze organophosphates and carbamates in a variety of vegetables at an appropriate detection level. Cholinesterase from cricket has the potential to be used for the development of a variety of methods for detecting residues, and the sensitivity could be enhanced by using new techniques in the future.

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Section: Introductionmentioning

confidence: 99%

Developing an In-House Colorimetric Method for Detecting Organophosphate and Carbamate Residue Using Cricket Cholinesterase

Wongta,

Hongsibsong,

Anand

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Developing an in-house colorimetric method for detecting organophosphate and carbamate residue using cricket cholinesterase

Wongta,

Hongsibsong,

Anand

et al. 2023

Chem. Biol. Technol. Agric.

View full text Add to dashboard Cite

Background Pesticides such as organophosphates and carbamates are used to control pests, leaving residues on fruits and vegetables. The assessment of residues in food materials is required for consumer and agricultural worker health protection. The purpose of this study was to develop an in-house colorimetric method for detecting organophosphate and carbamate residues using cricket cholinesterase that can be used as a low-cost, simple-to-use, and widely used test. Results The method’s parameters, including substrate specificity, kinetics, and incubation time, were optimized. The results indicated that acetylthiocholine iodide was more active than butyrylthiocholine iodide. Using a buffer with a pH of 8.0 resulted in the greatest activity after 5 min. The same enzyme activity was observed at 25 °C and 30 °C, which was greater than at 35 °C and 40 °C. In this study, the Km and Vmax values for cricket cholinesterase were 3.75 mM and 35 µM/min, respectively. The specificity and purification factor of the produced ChE were 1.54 µM/min/mg and 1.43-fold, respectively. The limit of detection for detecting organophosphates and carbamates was in the range of 0.002–0.877 ppm. The validity of the method yielded the following results: relative sensitivity of 95%, relative specificity of 90%, relative accuracy of 93%, positive predictive value of 95%, and negative predictive value of 90%. Conclusions The developed method is simple and inexpensive, and it can analyze organophosphates and carbamates in a variety of vegetables at an appropriate detection level. Cholinesterase from cricket has the potential to be used for the development of a variety of methods for detecting residues, and the sensitivity could be enhanced using new techniques in the future. Graphical Abstract

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Simple Colorimetric Method for Cholinesterase-inhibitor Screening in Gastric Content by Using Phytoesterase Enzyme from Kidney Bean

Cited by 2 publications

References 21 publications

Developing an In-House Colorimetric Method for Detecting Organophosphate and Carbamate Residue Using Cricket Cholinesterase

Developing an In-House Colorimetric Method for Detecting Organophosphate and Carbamate Residue Using Cricket Cholinesterase

Developing an in-house colorimetric method for detecting organophosphate and carbamate residue using cricket cholinesterase

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